2,4‐Dihydroxybenzoic Acid, a Novel SA Derivative, Controls Plant Immunity via UGT95B17‐Mediated Glucosylation: A Case Study in Camellia Sinensis
Abstract The plant hormone salicylic acid (SA) plays critical roles in plant innate immunity. Several SA derivatives and associated modification are identified, whereas the range and modes of action of SA‐related metabolites remain elusive. Here, the study discovered 2,4‐dihydroxybenzoic acid (2,4‐D...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-02-01
|
| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202307051 |
| _version_ | 1797305175880237056 |
|---|---|
| author | Mengqian Lu Yifan Zhao Yingying Feng Xiaoyan Tang Wei Zhao Keke Yu Yuting Pan Qiang Wang Jilai Cui Mengting Zhang Jieyang Jin Jingming Wang Mingyue Zhao Wilfried Schwab Chuankui Song |
| author_facet | Mengqian Lu Yifan Zhao Yingying Feng Xiaoyan Tang Wei Zhao Keke Yu Yuting Pan Qiang Wang Jilai Cui Mengting Zhang Jieyang Jin Jingming Wang Mingyue Zhao Wilfried Schwab Chuankui Song |
| author_sort | Mengqian Lu |
| collection | DOAJ |
| description | Abstract The plant hormone salicylic acid (SA) plays critical roles in plant innate immunity. Several SA derivatives and associated modification are identified, whereas the range and modes of action of SA‐related metabolites remain elusive. Here, the study discovered 2,4‐dihydroxybenzoic acid (2,4‐DHBA) and its glycosylated form as native SA derivatives in plants whose accumulation is largely induced by SA application and Ps. camelliae‐sinensis (Pcs) infection. CsSH1, a 4/5‐hydroxylase, catalyzes the hydroxylation of SA to 2,4‐DHBA, and UDP‐glucosyltransferase UGT95B17 catalyzes the formation of 2,4‐DHBA glucoside. Down‐regulation reduced the accumulation of 2,4‐DHBA glucosides and enhanced the sensitivity of tea plants to Pcs. Conversely, overexpression of UGT95B17 increased plant disease resistance. The exogenous application of 2,4‐DHBA and 2,5‐DHBA, as well as the accumulation of DHBA and plant resistance comparison, indicate that 2,4‐DHBA functions as a potentially bioactive molecule and is stored mainly as a glucose conjugate in tea plants, differs from the mechanism described in Arabidopsis. When 2,4‐DHBA is applied exogenously, UGT95B17‐silenced tea plants accumulated more 2,4‐DHBA than SA and showed induced resistance to Pcs infection. These results indicate that 2,4‐DHBA glucosylation positively regulates disease resistance and highlight the role of 2,4‐DHBA as potentially bioactive molecule in the establishment of basal resistance in tea plants. |
| first_indexed | 2024-03-08T00:21:19Z |
| format | Article |
| id | doaj.art-c919504e5ff640fa9a7cbbe361810a58 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-03-08T00:21:19Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-c919504e5ff640fa9a7cbbe361810a582024-02-16T08:29:37ZengWileyAdvanced Science2198-38442024-02-01117n/an/a10.1002/advs.2023070512,4‐Dihydroxybenzoic Acid, a Novel SA Derivative, Controls Plant Immunity via UGT95B17‐Mediated Glucosylation: A Case Study in Camellia SinensisMengqian Lu0Yifan Zhao1Yingying Feng2Xiaoyan Tang3Wei Zhao4Keke Yu5Yuting Pan6Qiang Wang7Jilai Cui8Mengting Zhang9Jieyang Jin10Jingming Wang11Mingyue Zhao12Wilfried Schwab13Chuankui Song14State Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaState Key Laboratory of Tea Plant Biology and Utilization International Joint Laboratory on Tea Chemistry and Health Effects Anhui Agricultural University Hefei Anhui 230036 P. R. ChinaAbstract The plant hormone salicylic acid (SA) plays critical roles in plant innate immunity. Several SA derivatives and associated modification are identified, whereas the range and modes of action of SA‐related metabolites remain elusive. Here, the study discovered 2,4‐dihydroxybenzoic acid (2,4‐DHBA) and its glycosylated form as native SA derivatives in plants whose accumulation is largely induced by SA application and Ps. camelliae‐sinensis (Pcs) infection. CsSH1, a 4/5‐hydroxylase, catalyzes the hydroxylation of SA to 2,4‐DHBA, and UDP‐glucosyltransferase UGT95B17 catalyzes the formation of 2,4‐DHBA glucoside. Down‐regulation reduced the accumulation of 2,4‐DHBA glucosides and enhanced the sensitivity of tea plants to Pcs. Conversely, overexpression of UGT95B17 increased plant disease resistance. The exogenous application of 2,4‐DHBA and 2,5‐DHBA, as well as the accumulation of DHBA and plant resistance comparison, indicate that 2,4‐DHBA functions as a potentially bioactive molecule and is stored mainly as a glucose conjugate in tea plants, differs from the mechanism described in Arabidopsis. When 2,4‐DHBA is applied exogenously, UGT95B17‐silenced tea plants accumulated more 2,4‐DHBA than SA and showed induced resistance to Pcs infection. These results indicate that 2,4‐DHBA glucosylation positively regulates disease resistance and highlight the role of 2,4‐DHBA as potentially bioactive molecule in the establishment of basal resistance in tea plants.https://doi.org/10.1002/advs.2023070512,4‐DHBACamellia sinensispathogen resistanceUDP‐glucosyltransferase |
| spellingShingle | Mengqian Lu Yifan Zhao Yingying Feng Xiaoyan Tang Wei Zhao Keke Yu Yuting Pan Qiang Wang Jilai Cui Mengting Zhang Jieyang Jin Jingming Wang Mingyue Zhao Wilfried Schwab Chuankui Song 2,4‐Dihydroxybenzoic Acid, a Novel SA Derivative, Controls Plant Immunity via UGT95B17‐Mediated Glucosylation: A Case Study in Camellia Sinensis Advanced Science 2,4‐DHBA Camellia sinensis pathogen resistance UDP‐glucosyltransferase |
| title | 2,4‐Dihydroxybenzoic Acid, a Novel SA Derivative, Controls Plant Immunity via UGT95B17‐Mediated Glucosylation: A Case Study in Camellia Sinensis |
| title_full | 2,4‐Dihydroxybenzoic Acid, a Novel SA Derivative, Controls Plant Immunity via UGT95B17‐Mediated Glucosylation: A Case Study in Camellia Sinensis |
| title_fullStr | 2,4‐Dihydroxybenzoic Acid, a Novel SA Derivative, Controls Plant Immunity via UGT95B17‐Mediated Glucosylation: A Case Study in Camellia Sinensis |
| title_full_unstemmed | 2,4‐Dihydroxybenzoic Acid, a Novel SA Derivative, Controls Plant Immunity via UGT95B17‐Mediated Glucosylation: A Case Study in Camellia Sinensis |
| title_short | 2,4‐Dihydroxybenzoic Acid, a Novel SA Derivative, Controls Plant Immunity via UGT95B17‐Mediated Glucosylation: A Case Study in Camellia Sinensis |
| title_sort | 2 4 dihydroxybenzoic acid a novel sa derivative controls plant immunity via ugt95b17 mediated glucosylation a case study in camellia sinensis |
| topic | 2,4‐DHBA Camellia sinensis pathogen resistance UDP‐glucosyltransferase |
| url | https://doi.org/10.1002/advs.202307051 |
| work_keys_str_mv | AT mengqianlu 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT yifanzhao 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT yingyingfeng 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT xiaoyantang 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT weizhao 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT kekeyu 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT yutingpan 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT qiangwang 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT jilaicui 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT mengtingzhang 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT jieyangjin 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT jingmingwang 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT mingyuezhao 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT wilfriedschwab 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis AT chuankuisong 24dihydroxybenzoicacidanovelsaderivativecontrolsplantimmunityviaugt95b17mediatedglucosylationacasestudyincamelliasinensis |