Evaluation of toxic effects of Coleus forskohlii extract on various body organs of experimental animals-rats
Background: The study aimed observation of pharmacological actions and any adverse effects on various body organs of rats with Coleus forskohlii (CF) extract. Aims and Objectives: The present study was undertaken to evaluate toxic effects of CF extract in experiments animals. Materials and Met...
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| Format: | Article |
| Language: | English |
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Manipal College of Medical Sciences, Pokhara
2022-12-01
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| Series: | Asian Journal of Medical Sciences |
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| Online Access: | https://www.nepjol.info/index.php/AJMS/article/view/49018 |
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| author | Haritha Yadav KC Yakaiah Vangoori Suresh Babu Sayana |
| author_facet | Haritha Yadav KC Yakaiah Vangoori Suresh Babu Sayana |
| author_sort | Haritha Yadav KC |
| collection | DOAJ |
| description | Background: The study aimed observation of pharmacological actions and any adverse effects on various body organs of rats with Coleus forskohlii (CF) extract.
Aims and Objectives: The present study was undertaken to evaluate toxic effects of CF extract in experiments animals.
Materials and Methods: In the single-dose oral toxicity study, groups of five healthy male and female rats were dosed orally with CF extract at 2000mg/kg BW as per organization of economic cooperation and development guidelines and were observed once daily for 14 consecutive days for toxicity, general behavior, and pharmacological effects. In sub-acute oral toxicity, the test substance was administered for 28 days with daily doses of 200, 400, and 800 mg/kg body weight. In subacute oral toxicity study, groups of five male and five female rats were subjected to 10% forskolin for 28 days. Initially autonomic symptoms (gross behavioral study), followed by hematological, biochemical, and histological parameters, were evaluated.
Results: No deaths were reported in all the toxicity studies performed. No significant changes were observed in the hematology and serum biochemistry values from the control group animals. The body weight changes and necropsy results were normal. There was no apparent progression of organ damage in any of these toxicity tests. Furthermore, CF extract did not produce any significant toxic effects in Wistar rats at 2000 mg/kg body weight and had no potential to induce organ damage.
Conclusion: The “no observed adverse effect level” of CF extract was determined up to 2000 mg/kg. |
| first_indexed | 2024-04-13T12:51:32Z |
| format | Article |
| id | doaj.art-c91e937400e54154b4ee21450db6a24f |
| institution | Directory Open Access Journal |
| issn | 2467-9100 2091-0576 |
| language | English |
| last_indexed | 2024-04-13T12:51:32Z |
| publishDate | 2022-12-01 |
| publisher | Manipal College of Medical Sciences, Pokhara |
| record_format | Article |
| series | Asian Journal of Medical Sciences |
| spelling | doaj.art-c91e937400e54154b4ee21450db6a24f2022-12-22T02:46:12ZengManipal College of Medical Sciences, PokharaAsian Journal of Medical Sciences2467-91002091-05762022-12-01131297103https://doi.org/10.3126/ajms.v13i12.49018Evaluation of toxic effects of Coleus forskohlii extract on various body organs of experimental animals-ratsHaritha Yadav KC 0https://orcid.org/0000-0003-0893-2243Yakaiah Vangoori 1Suresh Babu Sayana 2https://orcid.org/0000-0003-4971-4007Assistant Research Officer, Department of Pharmacology, Captain Srinivasa Murthy Central Ayurveda Research Institute, Chennai, Tamil Nadu, India Associate Professor, Department of Pharmacology, Mallareddy Medical College for Women, Hyderabad, IndiaAssistant Professor, Department of Pharmacology, Government Medical College, Suryapet, Telangana, India Background: The study aimed observation of pharmacological actions and any adverse effects on various body organs of rats with Coleus forskohlii (CF) extract. Aims and Objectives: The present study was undertaken to evaluate toxic effects of CF extract in experiments animals. Materials and Methods: In the single-dose oral toxicity study, groups of five healthy male and female rats were dosed orally with CF extract at 2000mg/kg BW as per organization of economic cooperation and development guidelines and were observed once daily for 14 consecutive days for toxicity, general behavior, and pharmacological effects. In sub-acute oral toxicity, the test substance was administered for 28 days with daily doses of 200, 400, and 800 mg/kg body weight. In subacute oral toxicity study, groups of five male and five female rats were subjected to 10% forskolin for 28 days. Initially autonomic symptoms (gross behavioral study), followed by hematological, biochemical, and histological parameters, were evaluated. Results: No deaths were reported in all the toxicity studies performed. No significant changes were observed in the hematology and serum biochemistry values from the control group animals. The body weight changes and necropsy results were normal. There was no apparent progression of organ damage in any of these toxicity tests. Furthermore, CF extract did not produce any significant toxic effects in Wistar rats at 2000 mg/kg body weight and had no potential to induce organ damage. Conclusion: The “no observed adverse effect level” of CF extract was determined up to 2000 mg/kg.https://www.nepjol.info/index.php/AJMS/article/view/49018coleus; gross behavior; acute and sub-acute; toxicity |
| spellingShingle | Haritha Yadav KC Yakaiah Vangoori Suresh Babu Sayana Evaluation of toxic effects of Coleus forskohlii extract on various body organs of experimental animals-rats Asian Journal of Medical Sciences coleus; gross behavior; acute and sub-acute; toxicity |
| title | Evaluation of toxic effects of Coleus forskohlii extract on various body organs of experimental animals-rats |
| title_full | Evaluation of toxic effects of Coleus forskohlii extract on various body organs of experimental animals-rats |
| title_fullStr | Evaluation of toxic effects of Coleus forskohlii extract on various body organs of experimental animals-rats |
| title_full_unstemmed | Evaluation of toxic effects of Coleus forskohlii extract on various body organs of experimental animals-rats |
| title_short | Evaluation of toxic effects of Coleus forskohlii extract on various body organs of experimental animals-rats |
| title_sort | evaluation of toxic effects of coleus forskohlii extract on various body organs of experimental animals rats |
| topic | coleus; gross behavior; acute and sub-acute; toxicity |
| url | https://www.nepjol.info/index.php/AJMS/article/view/49018 |
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