Effects of X-ray irradiation on the redox status of mouse colorectal tissues
The effects of X-ray irradiation on the redox status of colorectal tissues were investigated using magnetic resonance redox imaging.The pyrrolidine-ring nitroxyl contrast agent, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (CmP), was used as a suitable redox-sensitive contrast agent for colorec...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Advances in Redox Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667137923000073 |
| _version_ | 1827975079386939392 |
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| author | Megumi Ueno Raj Kumar Parajuli Hiromu Ito Megumi Iiyama Saaya Suzuki Ken-ichiro Matsumoto Ikuo Nakanishi Ichio Aoki Akira Sumiyoshi |
| author_facet | Megumi Ueno Raj Kumar Parajuli Hiromu Ito Megumi Iiyama Saaya Suzuki Ken-ichiro Matsumoto Ikuo Nakanishi Ichio Aoki Akira Sumiyoshi |
| author_sort | Megumi Ueno |
| collection | DOAJ |
| description | The effects of X-ray irradiation on the redox status of colorectal tissues were investigated using magnetic resonance redox imaging.The pyrrolidine-ring nitroxyl contrast agent, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (CmP), was used as a suitable redox-sensitive contrast agent for colorectal tissues. The lower abdomen of an 8-week-old female C3H/HeSlc mouse was X-ray irradiated with a single 10-Gy dose under anesthesia. X-rays were collimated through a 19-mm slit made by a pair of 5-cm-thick lead blocks. MRI experiments using CmP as the contrast agent were conducted on mice irradiated by X-rays at different times, i.e., 2‒8 h, 1, 2, 4, 8, 12, and 16 day(s) after irradiation. Dynamic datasets of multi-slice T1-weighted images were obtained by repeating scans every 20 s for 22 min. The time course of the CmP-induced T1-weighted signal in colorectal tissues was analyzed. The relationship between mitochondrial damage and redox responses was also investigated.The k1 decay rate of CmP, which mainly reflects the biological reduction of nitroxyl radicals in tissue, was significantly suppressed on day 2, increased on day 8, and then recovered to the control level. The decrease in the k1 value on day 2 indicated the inhibition of biological activity in injured tissue. Further animal studies are required to verify the relationship between the redox response of the colorectum tissue and the mitochondrial damages. This magnetic resonance redox imaging technique has the potential to visualize radio-biological responses in the tissues of living animals. |
| first_indexed | 2024-04-09T20:04:40Z |
| format | Article |
| id | doaj.art-c91f96c187594713827e5ae9e7fcb325 |
| institution | Directory Open Access Journal |
| issn | 2667-1379 |
| language | English |
| last_indexed | 2024-04-09T20:04:40Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Redox Research |
| spelling | doaj.art-c91f96c187594713827e5ae9e7fcb3252023-04-02T06:15:20ZengElsevierAdvances in Redox Research2667-13792023-07-018100067Effects of X-ray irradiation on the redox status of mouse colorectal tissuesMegumi Ueno0Raj Kumar Parajuli1Hiromu Ito2Megumi Iiyama3Saaya Suzuki4Ken-ichiro Matsumoto5Ikuo Nakanishi6Ichio Aoki7Akira Sumiyoshi8Quantitative RedOx Sensing Group, Department of Radiation Regulatory Science Research, National Institute of Radiological Sciences, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, JapanDepartment of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, JapanQuantum RedOx Chemistry Team, Institute for Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, JapanDepartment of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, JapanDepartment of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, JapanQuantitative RedOx Sensing Group, Department of Radiation Regulatory Science Research, National Institute of Radiological Sciences, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, Japan; Corresponding author.Quantum RedOx Chemistry Team, Institute for Quantum Life Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, JapanDepartment of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, JapanDepartment of Molecular Imaging and Theranostics, Institute for Quantum Medical Science, Quantum Life and Medical Science Directorate, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage-ku, Chiba-shi, Chiba 263-8555, JapanThe effects of X-ray irradiation on the redox status of colorectal tissues were investigated using magnetic resonance redox imaging.The pyrrolidine-ring nitroxyl contrast agent, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-N-oxyl (CmP), was used as a suitable redox-sensitive contrast agent for colorectal tissues. The lower abdomen of an 8-week-old female C3H/HeSlc mouse was X-ray irradiated with a single 10-Gy dose under anesthesia. X-rays were collimated through a 19-mm slit made by a pair of 5-cm-thick lead blocks. MRI experiments using CmP as the contrast agent were conducted on mice irradiated by X-rays at different times, i.e., 2‒8 h, 1, 2, 4, 8, 12, and 16 day(s) after irradiation. Dynamic datasets of multi-slice T1-weighted images were obtained by repeating scans every 20 s for 22 min. The time course of the CmP-induced T1-weighted signal in colorectal tissues was analyzed. The relationship between mitochondrial damage and redox responses was also investigated.The k1 decay rate of CmP, which mainly reflects the biological reduction of nitroxyl radicals in tissue, was significantly suppressed on day 2, increased on day 8, and then recovered to the control level. The decrease in the k1 value on day 2 indicated the inhibition of biological activity in injured tissue. Further animal studies are required to verify the relationship between the redox response of the colorectum tissue and the mitochondrial damages. This magnetic resonance redox imaging technique has the potential to visualize radio-biological responses in the tissues of living animals.http://www.sciencedirect.com/science/article/pii/S2667137923000073Ionizing radiationRadio-biological responseTissue redox responseMagnetic resonance redox imagingNitroxyl radicalRedox-sensitive contrast agent |
| spellingShingle | Megumi Ueno Raj Kumar Parajuli Hiromu Ito Megumi Iiyama Saaya Suzuki Ken-ichiro Matsumoto Ikuo Nakanishi Ichio Aoki Akira Sumiyoshi Effects of X-ray irradiation on the redox status of mouse colorectal tissues Advances in Redox Research Ionizing radiation Radio-biological response Tissue redox response Magnetic resonance redox imaging Nitroxyl radical Redox-sensitive contrast agent |
| title | Effects of X-ray irradiation on the redox status of mouse colorectal tissues |
| title_full | Effects of X-ray irradiation on the redox status of mouse colorectal tissues |
| title_fullStr | Effects of X-ray irradiation on the redox status of mouse colorectal tissues |
| title_full_unstemmed | Effects of X-ray irradiation on the redox status of mouse colorectal tissues |
| title_short | Effects of X-ray irradiation on the redox status of mouse colorectal tissues |
| title_sort | effects of x ray irradiation on the redox status of mouse colorectal tissues |
| topic | Ionizing radiation Radio-biological response Tissue redox response Magnetic resonance redox imaging Nitroxyl radical Redox-sensitive contrast agent |
| url | http://www.sciencedirect.com/science/article/pii/S2667137923000073 |
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