Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa Explants
The presence of deoxynivalenol (DON) in feed may increase intestinal barrier permeability. Disturbance of the intestinal barrier integrity may affect the absorption of antibiotics used in animals. Since the bioavailability of orally administered antibiotics significantly affects their efficacy and s...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/11/743 |
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| author | Marta Mendel Wojciech Karlik Urszula Latek Magdalena Chłopecka Ewelina Nowacka-Kozak Katarzyna Pietruszka Piotr Jedziniak |
| author_facet | Marta Mendel Wojciech Karlik Urszula Latek Magdalena Chłopecka Ewelina Nowacka-Kozak Katarzyna Pietruszka Piotr Jedziniak |
| author_sort | Marta Mendel |
| collection | DOAJ |
| description | The presence of deoxynivalenol (DON) in feed may increase intestinal barrier permeability. Disturbance of the intestinal barrier integrity may affect the absorption of antibiotics used in animals. Since the bioavailability of orally administered antibiotics significantly affects their efficacy and safety, it was decided to evaluate how DON influences the absorption of the most commonly used antibiotics in pigs, i.e., amoxicillin (AMX) and doxycycline (DOX). The studies were conducted using jejunal explants from adult pigs. Explants were incubated in Ussing chambers, in which a buffer containing DON (30 µg/mL), AMX (50 µg/mL), DOX (30 µg/mL), a combination of AMX + DON, or a combination of DOX + DON was used. Changes in transepithelial electrical resistance (TEER), the flux of transcellular and intracellular transport markers, and the flux of antibiotics across explants were measured. DON increased the permeability of small intestine explants, expressed by a reduction in TEER and an intensification of transcellular marker transport. DON did not affect AMX transport, but it accelerated DOX transport by approximately five times. The results suggest that DON inhibits the efflux transport of DOX to the intestinal lumen, and thus significantly changes its absorption from the gastrointestinal tract. |
| first_indexed | 2024-03-09T18:35:37Z |
| format | Article |
| id | doaj.art-c9202b9347e2466ca0d70ad3fc72b137 |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T18:35:37Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-c9202b9347e2466ca0d70ad3fc72b1372023-11-24T07:10:31ZengMDPI AGToxins2072-66512022-10-01141174310.3390/toxins14110743Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa ExplantsMarta Mendel0Wojciech Karlik1Urszula Latek2Magdalena Chłopecka3Ewelina Nowacka-Kozak4Katarzyna Pietruszka5Piotr Jedziniak6Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowka St. 166, 02-786 Warsaw, PolandInstitute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowka St. 166, 02-786 Warsaw, PolandInstitute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowka St. 166, 02-786 Warsaw, PolandInstitute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowka St. 166, 02-786 Warsaw, PolandDepartment of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, PolandDepartment of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, PolandDepartment of Pharmacology and Toxicology, National Veterinary Research Institute, Partyzantów 57, 24-100 Pulawy, PolandThe presence of deoxynivalenol (DON) in feed may increase intestinal barrier permeability. Disturbance of the intestinal barrier integrity may affect the absorption of antibiotics used in animals. Since the bioavailability of orally administered antibiotics significantly affects their efficacy and safety, it was decided to evaluate how DON influences the absorption of the most commonly used antibiotics in pigs, i.e., amoxicillin (AMX) and doxycycline (DOX). The studies were conducted using jejunal explants from adult pigs. Explants were incubated in Ussing chambers, in which a buffer containing DON (30 µg/mL), AMX (50 µg/mL), DOX (30 µg/mL), a combination of AMX + DON, or a combination of DOX + DON was used. Changes in transepithelial electrical resistance (TEER), the flux of transcellular and intracellular transport markers, and the flux of antibiotics across explants were measured. DON increased the permeability of small intestine explants, expressed by a reduction in TEER and an intensification of transcellular marker transport. DON did not affect AMX transport, but it accelerated DOX transport by approximately five times. The results suggest that DON inhibits the efflux transport of DOX to the intestinal lumen, and thus significantly changes its absorption from the gastrointestinal tract.https://www.mdpi.com/2072-6651/14/11/743deoxynivalenolamoxicillindoxycyclineUssing chamberswine jejunum mucosa explants |
| spellingShingle | Marta Mendel Wojciech Karlik Urszula Latek Magdalena Chłopecka Ewelina Nowacka-Kozak Katarzyna Pietruszka Piotr Jedziniak Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa Explants Toxins deoxynivalenol amoxicillin doxycycline Ussing chamber swine jejunum mucosa explants |
| title | Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa Explants |
| title_full | Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa Explants |
| title_fullStr | Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa Explants |
| title_full_unstemmed | Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa Explants |
| title_short | Does Deoxynivalenol Affect Amoxicillin and Doxycycline Absorption in the Gastrointestinal Tract? Ex Vivo Study on Swine Jejunum Mucosa Explants |
| title_sort | does deoxynivalenol affect amoxicillin and doxycycline absorption in the gastrointestinal tract ex vivo study on swine jejunum mucosa explants |
| topic | deoxynivalenol amoxicillin doxycycline Ussing chamber swine jejunum mucosa explants |
| url | https://www.mdpi.com/2072-6651/14/11/743 |
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