Co-existence of Multiple Resistance Mechanisms in Clinical Isolates of Carbapenem-Resistant Pseudomonas aeruginosa
Introduction: Multidrug resistance phenotype of Pseudomonas aeruginosa utilizes several resistant mechanisms to overcome the action of antibiotics. This phenotype is caused by several resistance mechanisms or a combination of thereof. This study aimed to evaluate various resistance mechanisms by ph...
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| Format: | Article |
| Language: | English |
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Bilimsel Tip Yayinevi
2022-03-01
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| Series: | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| Subjects: | |
| Online Access: | http://floradergisi.org/managete/fu_folder/2022-01/45-54%20Aylin%20Uskudar%20Guclu.pdf |
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| author | Aylin ÜSKÜDAR-GÜÇLÜ Hasan Cenk MİRZA Sezin ÜNLÜ |
| author_facet | Aylin ÜSKÜDAR-GÜÇLÜ Hasan Cenk MİRZA Sezin ÜNLÜ |
| author_sort | Aylin ÜSKÜDAR-GÜÇLÜ |
| collection | DOAJ |
| description | Introduction: Multidrug resistance phenotype of Pseudomonas aeruginosa utilizes several resistant mechanisms to overcome the action of antibiotics. This phenotype is caused by several resistance mechanisms or a combination of thereof. This study aimed to evaluate
various resistance mechanisms by phenotypic methods.
Materials and Methods: Carbapenem-resistant P. aeruginosa were included in this study. Antimicrobial resistance mechanisms such as efflux pump activity, reduced outer membrane permeability (OMP), various β-lactamase activities, and biofilm formation ability of clinical P. aeruginosa isolates were determined by phenotypic methods.
Results: Of the P. aeruginosa isolates, 33.7% (n= 33/98) had a positive efflux pump activity. The co-existence of positive efflux pump activity and Metallo β-lactamase (MBL) production was detected in 30.3% (10/33) of the isolates. In 34.7% of the clinical P. aeruginosa isolates, reduced OMP was detected and 70.6% of them were also biofilm producers. Totally 21.4% (21/98) of P. aeruginosa isolates were evaluated as extended-spectrum beta-lactamase (ESBL) positive. AmpC β-lactamase was detected in 15.3% (n= 15/98) of the clinical P. aeruginosa isolates. MBL activity was detected in 33.7% (n= 33/98) of the clinical P. aeruginosa isolates. Of the MBL-positive isolates, 69.7% were biofilm producers. The co-existence of MBL and reduced OMP was detected in 36.4% (n= 12/33).
Conclusion: High resistance of P. aeruginosa was attributed to several resistance mechanisms or a combination of thereof. This infections caused by multidrug-resistant (MDR) P. aeruginosa are difficult to treat due to the co-existence of different resistance mechanisms. |
| first_indexed | 2024-04-10T10:16:02Z |
| format | Article |
| id | doaj.art-c920eff117104bcaa2ec37949d426883 |
| institution | Directory Open Access Journal |
| issn | 1300-932X 1308-5115 |
| language | English |
| last_indexed | 2024-04-10T10:16:02Z |
| publishDate | 2022-03-01 |
| publisher | Bilimsel Tip Yayinevi |
| record_format | Article |
| series | Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi |
| spelling | doaj.art-c920eff117104bcaa2ec37949d4268832023-02-15T16:21:52ZengBilimsel Tip YayineviFlora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi1300-932X1308-51152022-03-01271455410.5578/flora.20229905Co-existence of Multiple Resistance Mechanisms in Clinical Isolates of Carbapenem-Resistant Pseudomonas aeruginosaAylin ÜSKÜDAR-GÜÇLÜ0https://orcid.org/0000-0002-1872-028XHasan Cenk MİRZA1https://orcid.org/0000-0002-8853-3893Sezin ÜNLÜ2https://orcid.org/0000-0001-7490-7981Department of Medical Microbiology, Başkent University Faculty of Medicine, Ankara, Turkey Department of Medical Microbiology, Başkent University Faculty of Medicine, Ankara, Turkey Department of Medical Microbiology, Başkent University Faculty of Medicine, Ankara, Turkey Introduction: Multidrug resistance phenotype of Pseudomonas aeruginosa utilizes several resistant mechanisms to overcome the action of antibiotics. This phenotype is caused by several resistance mechanisms or a combination of thereof. This study aimed to evaluate various resistance mechanisms by phenotypic methods. Materials and Methods: Carbapenem-resistant P. aeruginosa were included in this study. Antimicrobial resistance mechanisms such as efflux pump activity, reduced outer membrane permeability (OMP), various β-lactamase activities, and biofilm formation ability of clinical P. aeruginosa isolates were determined by phenotypic methods. Results: Of the P. aeruginosa isolates, 33.7% (n= 33/98) had a positive efflux pump activity. The co-existence of positive efflux pump activity and Metallo β-lactamase (MBL) production was detected in 30.3% (10/33) of the isolates. In 34.7% of the clinical P. aeruginosa isolates, reduced OMP was detected and 70.6% of them were also biofilm producers. Totally 21.4% (21/98) of P. aeruginosa isolates were evaluated as extended-spectrum beta-lactamase (ESBL) positive. AmpC β-lactamase was detected in 15.3% (n= 15/98) of the clinical P. aeruginosa isolates. MBL activity was detected in 33.7% (n= 33/98) of the clinical P. aeruginosa isolates. Of the MBL-positive isolates, 69.7% were biofilm producers. The co-existence of MBL and reduced OMP was detected in 36.4% (n= 12/33). Conclusion: High resistance of P. aeruginosa was attributed to several resistance mechanisms or a combination of thereof. This infections caused by multidrug-resistant (MDR) P. aeruginosa are difficult to treat due to the co-existence of different resistance mechanisms.http://floradergisi.org/managete/fu_folder/2022-01/45-54%20Aylin%20Uskudar%20Guclu.pdfp. aeruginosabiofilmantimicrobial resistance mechanismscarbapenem resistance |
| spellingShingle | Aylin ÜSKÜDAR-GÜÇLÜ Hasan Cenk MİRZA Sezin ÜNLÜ Co-existence of Multiple Resistance Mechanisms in Clinical Isolates of Carbapenem-Resistant Pseudomonas aeruginosa Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi p. aeruginosa biofilm antimicrobial resistance mechanisms carbapenem resistance |
| title | Co-existence of Multiple Resistance Mechanisms in Clinical Isolates of Carbapenem-Resistant Pseudomonas aeruginosa |
| title_full | Co-existence of Multiple Resistance Mechanisms in Clinical Isolates of Carbapenem-Resistant Pseudomonas aeruginosa |
| title_fullStr | Co-existence of Multiple Resistance Mechanisms in Clinical Isolates of Carbapenem-Resistant Pseudomonas aeruginosa |
| title_full_unstemmed | Co-existence of Multiple Resistance Mechanisms in Clinical Isolates of Carbapenem-Resistant Pseudomonas aeruginosa |
| title_short | Co-existence of Multiple Resistance Mechanisms in Clinical Isolates of Carbapenem-Resistant Pseudomonas aeruginosa |
| title_sort | co existence of multiple resistance mechanisms in clinical isolates of carbapenem resistant pseudomonas aeruginosa |
| topic | p. aeruginosa biofilm antimicrobial resistance mechanisms carbapenem resistance |
| url | http://floradergisi.org/managete/fu_folder/2022-01/45-54%20Aylin%20Uskudar%20Guclu.pdf |
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