Metabolically versatile psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H12B is an efficient producer of siderophores and accompanying metabolites (SAM) useful for agricultural purposes
Abstract Background Bacterial siderophores are chelating compounds with the potential of application in agriculture, due to their plant growth-promoting (PGP) properties, however, high production and purification costs are limiting factors for their wider application. Cost-efficiency of the producti...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | Microbial Cell Factories |
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| Online Access: | https://doi.org/10.1186/s12934-023-02105-2 |
| _version_ | 1797836174832697344 |
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| author | M. Musialowski Ł. Kowalewska R. Stasiuk T. Krucoń K. Debiec-Andrzejewska |
| author_facet | M. Musialowski Ł. Kowalewska R. Stasiuk T. Krucoń K. Debiec-Andrzejewska |
| author_sort | M. Musialowski |
| collection | DOAJ |
| description | Abstract Background Bacterial siderophores are chelating compounds with the potential of application in agriculture, due to their plant growth-promoting (PGP) properties, however, high production and purification costs are limiting factors for their wider application. Cost-efficiency of the production could be increased by omitting purification processes, especially since siderophores accompanying metabolites (SAM) often also possess PGP traits. In this study, the metabolism versatility of Pseudomonas sp. ANT_H12B was used for the optimization of siderophores production and the potential of these metabolites and SAM was characterized in the context of PGP properties. Results The metabolic diversity of ANT_H12B was examined through genomic analysis and phenotype microarrays. The strain was found to be able to use numerous C, N, P, and S sources, which allowed for the design of novel media suitable for efficient production of siderophores in the form of pyoverdine (223.50–512.60 μM). Moreover, depending on the culture medium, the pH of the siderophores and SAM solutions varied from acidic (pH < 5) to alkaline (pH > 8). In a germination test, siderophores and SAM were shown to have a positive effect on plants, with a significant increase in germination percentage observed in beetroot, pea, and tobacco. The PGP potential of SAM was further elucidated through GC/MS analysis, which revealed other compounds with PGP potential, such as indolic acetic acids, organic acids, fatty acids, sugars and alcohols. These compounds not only improved seed germination but could also potentially be beneficial for plant fitness and soil quality. Conclusions Pseudomonas sp. ANT_H12B was presented as an efficient producer of siderophores and SAM which exhibit PGP potential. It was also shown that omitting downstream processes could not only limit the costs of siderophores production but also improve their agricultural potential. |
| first_indexed | 2024-04-09T15:05:31Z |
| format | Article |
| id | doaj.art-c9228154baac473dbb6616042712c8b7 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-09T15:05:31Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-c9228154baac473dbb6616042712c8b72023-04-30T11:32:21ZengBMCMicrobial Cell Factories1475-28592023-04-0122111610.1186/s12934-023-02105-2Metabolically versatile psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H12B is an efficient producer of siderophores and accompanying metabolites (SAM) useful for agricultural purposesM. Musialowski0Ł. Kowalewska1R. Stasiuk2T. Krucoń3K. Debiec-Andrzejewska4Department of Geomicrobiology, Institute of Microbiology, Faculty of Biology, University of WarsawDepartment of Plant Anatomy and Cytology, Institute of Experimental Plant Biology and Biotechnology, Faculty of Biology, University of WarsawDepartment of Geomicrobiology, Institute of Microbiology, Faculty of Biology, University of WarsawDepartment of Environmental Microbiology and Biotechnology, Institute of Microbiology, Faculty of Biology, University of WarsawDepartment of Geomicrobiology, Institute of Microbiology, Faculty of Biology, University of WarsawAbstract Background Bacterial siderophores are chelating compounds with the potential of application in agriculture, due to their plant growth-promoting (PGP) properties, however, high production and purification costs are limiting factors for their wider application. Cost-efficiency of the production could be increased by omitting purification processes, especially since siderophores accompanying metabolites (SAM) often also possess PGP traits. In this study, the metabolism versatility of Pseudomonas sp. ANT_H12B was used for the optimization of siderophores production and the potential of these metabolites and SAM was characterized in the context of PGP properties. Results The metabolic diversity of ANT_H12B was examined through genomic analysis and phenotype microarrays. The strain was found to be able to use numerous C, N, P, and S sources, which allowed for the design of novel media suitable for efficient production of siderophores in the form of pyoverdine (223.50–512.60 μM). Moreover, depending on the culture medium, the pH of the siderophores and SAM solutions varied from acidic (pH < 5) to alkaline (pH > 8). In a germination test, siderophores and SAM were shown to have a positive effect on plants, with a significant increase in germination percentage observed in beetroot, pea, and tobacco. The PGP potential of SAM was further elucidated through GC/MS analysis, which revealed other compounds with PGP potential, such as indolic acetic acids, organic acids, fatty acids, sugars and alcohols. These compounds not only improved seed germination but could also potentially be beneficial for plant fitness and soil quality. Conclusions Pseudomonas sp. ANT_H12B was presented as an efficient producer of siderophores and SAM which exhibit PGP potential. It was also shown that omitting downstream processes could not only limit the costs of siderophores production but also improve their agricultural potential.https://doi.org/10.1186/s12934-023-02105-2SiderophoresPyoverdinePsychrotolerant bacteriaPlant-growth promotionAgricultureBacterial metabolites |
| spellingShingle | M. Musialowski Ł. Kowalewska R. Stasiuk T. Krucoń K. Debiec-Andrzejewska Metabolically versatile psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H12B is an efficient producer of siderophores and accompanying metabolites (SAM) useful for agricultural purposes Microbial Cell Factories Siderophores Pyoverdine Psychrotolerant bacteria Plant-growth promotion Agriculture Bacterial metabolites |
| title | Metabolically versatile psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H12B is an efficient producer of siderophores and accompanying metabolites (SAM) useful for agricultural purposes |
| title_full | Metabolically versatile psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H12B is an efficient producer of siderophores and accompanying metabolites (SAM) useful for agricultural purposes |
| title_fullStr | Metabolically versatile psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H12B is an efficient producer of siderophores and accompanying metabolites (SAM) useful for agricultural purposes |
| title_full_unstemmed | Metabolically versatile psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H12B is an efficient producer of siderophores and accompanying metabolites (SAM) useful for agricultural purposes |
| title_short | Metabolically versatile psychrotolerant Antarctic bacterium Pseudomonas sp. ANT_H12B is an efficient producer of siderophores and accompanying metabolites (SAM) useful for agricultural purposes |
| title_sort | metabolically versatile psychrotolerant antarctic bacterium pseudomonas sp ant h12b is an efficient producer of siderophores and accompanying metabolites sam useful for agricultural purposes |
| topic | Siderophores Pyoverdine Psychrotolerant bacteria Plant-growth promotion Agriculture Bacterial metabolites |
| url | https://doi.org/10.1186/s12934-023-02105-2 |
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