Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR
Canine parvovirus (CPV) is a pathogen causing hemorrhagic enteritis in puppies and mainly transmitting via feco-oral route. In this study, stool samples were collected from a total of 35 animals suspected of CPV. The samples were examined by Conventional PCR, Nested PCR and Differential PCR tests. 2...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Turkish Science and Technology Publishing (TURSTEP)
2022-02-01
|
| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4653 |
| _version_ | 1797916936806334464 |
|---|---|
| author | Sibel Hasırcıoğlu Hatice Pelin Aslım |
| author_facet | Sibel Hasırcıoğlu Hatice Pelin Aslım |
| author_sort | Sibel Hasırcıoğlu |
| collection | DOAJ |
| description | Canine parvovirus (CPV) is a pathogen causing hemorrhagic enteritis in puppies and mainly transmitting via feco-oral route. In this study, stool samples were collected from a total of 35 animals suspected of CPV. The samples were examined by Conventional PCR, Nested PCR and Differential PCR tests. 20 out of 35 dogs (57.1%) were detected positive by conventional PCR, 31 (88.6%) by nested PCR and 30 (85.7%) by differential PCR. CPV 2a was stated as the most common antigenic type, male animals and 0–3-month-olds had a high rate of becoming sick and vaccinated animals might also catch the disease, rarely. Accordingly, it is recommended to focus on studies providing molecular epidemiology surveillance in order to detect the existing subtypes and develop reliable diagnosis and vaccination methods. |
| first_indexed | 2024-04-10T13:05:17Z |
| format | Article |
| id | doaj.art-c9257b0c48564c2ab534e1c19a9fb50d |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T13:05:17Z |
| publishDate | 2022-02-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-c9257b0c48564c2ab534e1c19a9fb50d2023-02-15T16:12:58ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2022-02-0110225425910.24925/turjaf.v10i2.254-259.46532383Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCRSibel Hasırcıoğlu0Hatice Pelin Aslım1Department of Virology, Faculty of Veterinary, University of Selcuk, 42130 KonyaDepartment of Virology, Faculty of Veterinary, University of Selcuk, 42130 KonyaCanine parvovirus (CPV) is a pathogen causing hemorrhagic enteritis in puppies and mainly transmitting via feco-oral route. In this study, stool samples were collected from a total of 35 animals suspected of CPV. The samples were examined by Conventional PCR, Nested PCR and Differential PCR tests. 20 out of 35 dogs (57.1%) were detected positive by conventional PCR, 31 (88.6%) by nested PCR and 30 (85.7%) by differential PCR. CPV 2a was stated as the most common antigenic type, male animals and 0–3-month-olds had a high rate of becoming sick and vaccinated animals might also catch the disease, rarely. Accordingly, it is recommended to focus on studies providing molecular epidemiology surveillance in order to detect the existing subtypes and develop reliable diagnosis and vaccination methods.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4653cpvpcrantigenic characterizationdifferential pcrnested pcr |
| spellingShingle | Sibel Hasırcıoğlu Hatice Pelin Aslım Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR Turkish Journal of Agriculture: Food Science and Technology cpv pcr antigenic characterization differential pcr nested pcr |
| title | Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR |
| title_full | Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR |
| title_fullStr | Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR |
| title_full_unstemmed | Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR |
| title_short | Comparative Detection of Canine Parvovirus by Differential PCR, Conventional PCR and Nested PCR tests: Detecting Antigenic Variants by Differential PCR |
| title_sort | comparative detection of canine parvovirus by differential pcr conventional pcr and nested pcr tests detecting antigenic variants by differential pcr |
| topic | cpv pcr antigenic characterization differential pcr nested pcr |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/4653 |
| work_keys_str_mv | AT sibelhasırcıoglu comparativedetectionofcanineparvovirusbydifferentialpcrconventionalpcrandnestedpcrtestsdetectingantigenicvariantsbydifferentialpcr AT haticepelinaslım comparativedetectionofcanineparvovirusbydifferentialpcrconventionalpcrandnestedpcrtestsdetectingantigenicvariantsbydifferentialpcr |