Saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profile
IntroductionSaccharomyces boulardii (S. boulardii) has shown clinical beneficial effect in inflammatory bowel diseases recently. However, the underlying mechanisms remain incompletely understood. The aim of present study was to tested whether S. boulardii targets gut microbiota to protect against th...
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Frontiers Media S.A.
2023-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1204122/full |
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| author | Hui Gao Yinzheng Li Jiqu Xu Jiqu Xu Xuezhi Zuo Tiantian Yue Huzi Xu Jie Sun Meng Wang Ting Ye Yan Yu Ying Yao Ying Yao |
| author_facet | Hui Gao Yinzheng Li Jiqu Xu Jiqu Xu Xuezhi Zuo Tiantian Yue Huzi Xu Jie Sun Meng Wang Ting Ye Yan Yu Ying Yao Ying Yao |
| author_sort | Hui Gao |
| collection | DOAJ |
| description | IntroductionSaccharomyces boulardii (S. boulardii) has shown clinical beneficial effect in inflammatory bowel diseases recently. However, the underlying mechanisms remain incompletely understood. The aim of present study was to tested whether S. boulardii targets gut microbiota to protect against the development of experimental colitis in mice.MethodsFemale C57BL/6 mice were gavaged with S. boulardii for 3 weeks before being challenged with dextran sulphate sodium to induce ulcerative colitis. Bodyweight, diarrhea severity, intestinal permeability, colonic histopathology, colonic inflammatory status, and epithelial cell death of mice were examined. The fecal microbiota and its metabolomic profiles were detected by 16S rDNA sequencing and UPLC-MS, respectively.Results and DiscussionSupplementation with S. boulardii significantly prevented weight loss and colon shortening, lowered colonic inflammation, ameliorated epithelial injury, and enhanced the intestinal barrier integrity in colitis mice. By inhibiting the abundance of pathogenic bacteria and increasing the probiotics abundance, S. boulardii improved the microbial diversity and restored the microbiota dysbiosis. Moreover, it also modulated microbial metabolome and altered the relative contents of metabolites involving amino acids, lipids, energy and vitamin metabolisms. These yeast-driven shifts in gut flora and metabolites are were associated with each other and with the inflammation profile in colitis. Collectively, S. boulardii exerts protective effects on colitis in mice by reshaping gut microbiome and its metabolic profile, indicating it as a promising therapeutic avenue. |
| first_indexed | 2024-03-13T00:33:17Z |
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| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-13T00:33:17Z |
| publishDate | 2023-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-c9286ae7cb1647e79e172d5882ec17c02023-07-10T09:18:41ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-07-011410.3389/fmicb.2023.12041221204122Saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profileHui Gao0Yinzheng Li1Jiqu Xu2Jiqu Xu3Xuezhi Zuo4Tiantian Yue5Huzi Xu6Jie Sun7Meng Wang8Ting Ye9Yan Yu10Ying Yao11Ying Yao12Department of Clinical Nutrition, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Nutriology, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, ChinaHubei Key Laboratory of Lipid Chemistry and Nutrition, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, ChinaDepartment of Clinical Nutrition, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Clinical Nutrition, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Clinical Nutrition, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Clinical Nutrition, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Gastroenterology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Clinical Nutrition, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaDepartment of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, ChinaIntroductionSaccharomyces boulardii (S. boulardii) has shown clinical beneficial effect in inflammatory bowel diseases recently. However, the underlying mechanisms remain incompletely understood. The aim of present study was to tested whether S. boulardii targets gut microbiota to protect against the development of experimental colitis in mice.MethodsFemale C57BL/6 mice were gavaged with S. boulardii for 3 weeks before being challenged with dextran sulphate sodium to induce ulcerative colitis. Bodyweight, diarrhea severity, intestinal permeability, colonic histopathology, colonic inflammatory status, and epithelial cell death of mice were examined. The fecal microbiota and its metabolomic profiles were detected by 16S rDNA sequencing and UPLC-MS, respectively.Results and DiscussionSupplementation with S. boulardii significantly prevented weight loss and colon shortening, lowered colonic inflammation, ameliorated epithelial injury, and enhanced the intestinal barrier integrity in colitis mice. By inhibiting the abundance of pathogenic bacteria and increasing the probiotics abundance, S. boulardii improved the microbial diversity and restored the microbiota dysbiosis. Moreover, it also modulated microbial metabolome and altered the relative contents of metabolites involving amino acids, lipids, energy and vitamin metabolisms. These yeast-driven shifts in gut flora and metabolites are were associated with each other and with the inflammation profile in colitis. Collectively, S. boulardii exerts protective effects on colitis in mice by reshaping gut microbiome and its metabolic profile, indicating it as a promising therapeutic avenue.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1204122/fullSaccharomyces boulardiiulcerative colitisgut microbiotametabolomicscorrelation |
| spellingShingle | Hui Gao Yinzheng Li Jiqu Xu Jiqu Xu Xuezhi Zuo Tiantian Yue Huzi Xu Jie Sun Meng Wang Ting Ye Yan Yu Ying Yao Ying Yao Saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profile Frontiers in Microbiology Saccharomyces boulardii ulcerative colitis gut microbiota metabolomics correlation |
| title | Saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profile |
| title_full | Saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profile |
| title_fullStr | Saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profile |
| title_full_unstemmed | Saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profile |
| title_short | Saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profile |
| title_sort | saccharomyces boulardii protects against murine experimental colitis by reshaping the gut microbiome and its metabolic profile |
| topic | Saccharomyces boulardii ulcerative colitis gut microbiota metabolomics correlation |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1204122/full |
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