Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis
Objective(s): Blood-brain barrier (BBB) permeability is central in multiple sclerosis (MS) pathophysiology, and exercise may improve BBB integrity. The current study investigated the prophylactic and/ or therapeutic role of aerobic exercise (EX) training on BBB integrity in experimental autoimmune e...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2022-02-01
|
| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_19659_3256d319ee88474e14318781a63eaff3.pdf |
| _version_ | 1819059750022676480 |
|---|---|
| author | Omid Razi Abdolhossein Parnow Iraj Rashidi Nafiseh Pakravan Seyed Ershad Nedaei Robert Motl |
| author_facet | Omid Razi Abdolhossein Parnow Iraj Rashidi Nafiseh Pakravan Seyed Ershad Nedaei Robert Motl |
| author_sort | Omid Razi |
| collection | DOAJ |
| description | Objective(s): Blood-brain barrier (BBB) permeability is central in multiple sclerosis (MS) pathophysiology, and exercise may improve BBB integrity. The current study investigated the prophylactic and/ or therapeutic role of aerobic exercise (EX) training on BBB integrity in experimental autoimmune encephalomyelitis (EAE). Materials and Methods: Forty female Lewis rats were randomly divided into four groups. The experimental groups included: no-EAE induction+ no-exercise (no-EAE+ no-EX), no-EAE induction+ exercise (no-EAE+EX), EAE induction+ no-exercise (EAE+ no-EX), and EAE induction+ exercise (EAE+EX). The no-EAE+EX and EAE+EX groups performed six weeks of progressive aerobic exercise training. GFAP, angiopoietin 1 (Ang-1) expression, tight-junction (TJ) proteins of claudin-5 and occludin were measured as components of BBB integrity and the rate of neuronal apoptosis was evaluated in hippocampi. Results: A significant increase in GFAP and Ang-1 expression (P<0.001) and conversely a down-regulation in TJ proteins (P<0.05) was found in the brains of the no-EAE+EX group compared with the no-EAE+ no-EX group. The expression of GFAP and Ang-1 proteins significantly increased in the hippocampi of the EAE+ no-EX group (P<0.001), whereas aerobic training (in the EAE+EX group) meaningfully reversed such increases (P<0.001). Besides, down-regulated TJ proteins and increased neuronal apoptosis induced by EAE induction (EAE+ no-EX group) were restored and reduced, respectively, by aerobic training in the CNS of the EAE+EX group (P<0.001). Conclusion: The provision of a six-week treadmill aerobic training buffered the detrimental effects of EAE on BBB integrity and consequently neuronal apoptosis. |
| first_indexed | 2024-12-21T14:16:03Z |
| format | Article |
| id | doaj.art-c929b634c61c4ce3b3eb23fadac82a6c |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-21T14:16:03Z |
| publishDate | 2022-02-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-c929b634c61c4ce3b3eb23fadac82a6c2022-12-21T19:00:55ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-02-0125224525310.22038/ijbms.2022.61671.1364519659Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitisOmid Razi0Abdolhossein Parnow1Iraj Rashidi2Nafiseh Pakravan3Seyed Ershad Nedaei4Robert Motl5Department of Exercise Physiology, Faculty of Physical Education and Sports Science, Razi University, Kermanshah, IranDepartment of Exercise Physiology, Faculty of Physical Education and Sports Science, Razi University, Kermanshah, IranDepartment of Anatomical Sciences, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Immunology, School of Medicine, Alborz University of Medical Science, Karaj, IranDepartment of Physiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, IranDepartment of Kinesiology and Nutrition, College of Applied Health Sciences, University of Illinois Chicago, United States of AmericaObjective(s): Blood-brain barrier (BBB) permeability is central in multiple sclerosis (MS) pathophysiology, and exercise may improve BBB integrity. The current study investigated the prophylactic and/ or therapeutic role of aerobic exercise (EX) training on BBB integrity in experimental autoimmune encephalomyelitis (EAE). Materials and Methods: Forty female Lewis rats were randomly divided into four groups. The experimental groups included: no-EAE induction+ no-exercise (no-EAE+ no-EX), no-EAE induction+ exercise (no-EAE+EX), EAE induction+ no-exercise (EAE+ no-EX), and EAE induction+ exercise (EAE+EX). The no-EAE+EX and EAE+EX groups performed six weeks of progressive aerobic exercise training. GFAP, angiopoietin 1 (Ang-1) expression, tight-junction (TJ) proteins of claudin-5 and occludin were measured as components of BBB integrity and the rate of neuronal apoptosis was evaluated in hippocampi. Results: A significant increase in GFAP and Ang-1 expression (P<0.001) and conversely a down-regulation in TJ proteins (P<0.05) was found in the brains of the no-EAE+EX group compared with the no-EAE+ no-EX group. The expression of GFAP and Ang-1 proteins significantly increased in the hippocampi of the EAE+ no-EX group (P<0.001), whereas aerobic training (in the EAE+EX group) meaningfully reversed such increases (P<0.001). Besides, down-regulated TJ proteins and increased neuronal apoptosis induced by EAE induction (EAE+ no-EX group) were restored and reduced, respectively, by aerobic training in the CNS of the EAE+EX group (P<0.001). Conclusion: The provision of a six-week treadmill aerobic training buffered the detrimental effects of EAE on BBB integrity and consequently neuronal apoptosis.https://ijbms.mums.ac.ir/article_19659_3256d319ee88474e14318781a63eaff3.pdfaerobic trainingastrogliosisbrain barrier dysfunctionclaudin-5eae modeloccludin |
| spellingShingle | Omid Razi Abdolhossein Parnow Iraj Rashidi Nafiseh Pakravan Seyed Ershad Nedaei Robert Motl Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis Iranian Journal of Basic Medical Sciences aerobic training astrogliosis brain barrier dysfunction claudin-5 eae model occludin |
| title | Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis |
| title_full | Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis |
| title_fullStr | Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis |
| title_full_unstemmed | Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis |
| title_short | Aerobic training improves blood-brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis |
| title_sort | aerobic training improves blood brain barrier and neuronal apoptosis in experimental autoimmune encephalomyelitis |
| topic | aerobic training astrogliosis brain barrier dysfunction claudin-5 eae model occludin |
| url | https://ijbms.mums.ac.ir/article_19659_3256d319ee88474e14318781a63eaff3.pdf |
| work_keys_str_mv | AT omidrazi aerobictrainingimprovesbloodbrainbarrierandneuronalapoptosisinexperimentalautoimmuneencephalomyelitis AT abdolhosseinparnow aerobictrainingimprovesbloodbrainbarrierandneuronalapoptosisinexperimentalautoimmuneencephalomyelitis AT irajrashidi aerobictrainingimprovesbloodbrainbarrierandneuronalapoptosisinexperimentalautoimmuneencephalomyelitis AT nafisehpakravan aerobictrainingimprovesbloodbrainbarrierandneuronalapoptosisinexperimentalautoimmuneencephalomyelitis AT seyedershadnedaei aerobictrainingimprovesbloodbrainbarrierandneuronalapoptosisinexperimentalautoimmuneencephalomyelitis AT robertmotl aerobictrainingimprovesbloodbrainbarrierandneuronalapoptosisinexperimentalautoimmuneencephalomyelitis |