IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa
IZUMO1 is an acrosome transmembrane protein implicated in the adhesion and fusion of gametes. This study aims to describe the distribution of IZUMO1 in human sperm under different physiological conditions: before capacitation (NCS), at one-hour capacitation (CS1), after a hyaluronic acid (HA) select...
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MDPI AG
2023-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/11/2872 |
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| author | María José Gómez-Torres Miranda Hernández-Falcó Andrea López-Botella Natalia Huerta-Retamal Paula Sáez-Espinosa |
| author_facet | María José Gómez-Torres Miranda Hernández-Falcó Andrea López-Botella Natalia Huerta-Retamal Paula Sáez-Espinosa |
| author_sort | María José Gómez-Torres |
| collection | DOAJ |
| description | IZUMO1 is an acrosome transmembrane protein implicated in the adhesion and fusion of gametes. This study aims to describe the distribution of IZUMO1 in human sperm under different physiological conditions: before capacitation (NCS), at one-hour capacitation (CS1), after a hyaluronic acid (HA) selection test (mature, MS1 and immature, IS1), and induced acrosome reaction from one-hour-capacitated sperm (ARS1). The data obtained in NCS, CS1, and MS1 significantly highlight dotted fluorescence in the acrosomal region (P1) as the major staining pattern (~70%). Moreover, we describe a new distribution pattern (P2) with a dotted acrosomal region and a labelled equatorial region that significantly increases in HA-bound spermatozoa, suggesting the onset of the migration of IZUMO1. In contrast, unbound spermatozoa presented an increase in P3 (equatorial region labelled) and P4 (not labelled). Finally, costaining to observe IZUMO1 distribution and acrosome status was performed in ARS1. Interestingly, we reported a variety of combinations between the IZUMO1 staining patterns and the acrosomal stages. In conclusion, these data show as a novelty the diffusion of the IZUMO1 protein during different physiological conditions that could contribute to the improvement in sperm selection techniques. |
| first_indexed | 2024-03-09T17:00:57Z |
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| id | doaj.art-c92ccb8c4cb14b5cb74eeb73699a1c34 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T17:00:57Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-c92ccb8c4cb14b5cb74eeb73699a1c342023-11-24T14:30:32ZengMDPI AGBiomedicines2227-90592023-10-011111287210.3390/biomedicines11112872IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human SpermatozoaMaría José Gómez-Torres0Miranda Hernández-Falcó1Andrea López-Botella2Natalia Huerta-Retamal3Paula Sáez-Espinosa4Department of Biotechnology, University of Alicante, 03690 Alicante, SpainDepartment of Biotechnology, University of Alicante, 03690 Alicante, SpainDepartment of Biotechnology, University of Alicante, 03690 Alicante, SpainDepartment of Biotechnology, University of Alicante, 03690 Alicante, SpainDepartment of Biotechnology, University of Alicante, 03690 Alicante, SpainIZUMO1 is an acrosome transmembrane protein implicated in the adhesion and fusion of gametes. This study aims to describe the distribution of IZUMO1 in human sperm under different physiological conditions: before capacitation (NCS), at one-hour capacitation (CS1), after a hyaluronic acid (HA) selection test (mature, MS1 and immature, IS1), and induced acrosome reaction from one-hour-capacitated sperm (ARS1). The data obtained in NCS, CS1, and MS1 significantly highlight dotted fluorescence in the acrosomal region (P1) as the major staining pattern (~70%). Moreover, we describe a new distribution pattern (P2) with a dotted acrosomal region and a labelled equatorial region that significantly increases in HA-bound spermatozoa, suggesting the onset of the migration of IZUMO1. In contrast, unbound spermatozoa presented an increase in P3 (equatorial region labelled) and P4 (not labelled). Finally, costaining to observe IZUMO1 distribution and acrosome status was performed in ARS1. Interestingly, we reported a variety of combinations between the IZUMO1 staining patterns and the acrosomal stages. In conclusion, these data show as a novelty the diffusion of the IZUMO1 protein during different physiological conditions that could contribute to the improvement in sperm selection techniques.https://www.mdpi.com/2227-9059/11/11/2872acrosome reactiongamete fusionIZUMO1sperm selectionmale infertilityimmunofluorescent |
| spellingShingle | María José Gómez-Torres Miranda Hernández-Falcó Andrea López-Botella Natalia Huerta-Retamal Paula Sáez-Espinosa IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa Biomedicines acrosome reaction gamete fusion IZUMO1 sperm selection male infertility immunofluorescent |
| title | IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa |
| title_full | IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa |
| title_fullStr | IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa |
| title_full_unstemmed | IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa |
| title_short | IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa |
| title_sort | izumo1 receptor localization during hyaluronic acid selection in human spermatozoa |
| topic | acrosome reaction gamete fusion IZUMO1 sperm selection male infertility immunofluorescent |
| url | https://www.mdpi.com/2227-9059/11/11/2872 |
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