Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues
As a cellular-assembly technique, bioprinting has been extensively used in tissue engineering and regenerative medicine to construct hydrogel-based three-dimensional (3D) tissue-like models with prescribed geometry. Here, we introduced a unique direct-write bioprinting strategy to fabricate a bilaye...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2019.00478/full |
| _version_ | 1818906628406116352 |
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| author | Elahe Masaeli Elahe Masaeli Christophe Marquette |
| author_facet | Elahe Masaeli Elahe Masaeli Christophe Marquette |
| author_sort | Elahe Masaeli |
| collection | DOAJ |
| description | As a cellular-assembly technique, bioprinting has been extensively used in tissue engineering and regenerative medicine to construct hydrogel-based three-dimensional (3D) tissue-like models with prescribed geometry. Here, we introduced a unique direct-write bioprinting strategy to fabricate a bilayer flat tissue in a hydrogel-free approach. A printed retina pigmented epithelium layer (RPE) was applied as living biopaper for positioning a fibroblast layer without using any hydrogel in bioink. We adjusted the number of cells in the inkjet droplets in order to obtain a uniform printed cell layer and demonstrated the formation of a bilayer construct through confocal imaging. Since our printing system introduced low levels of shear stress to the cells, it did not have a negative effect on cell survival, although cell viability was generally lower than that of control group over 1 week post-printing. In conclusion, our novel direct-write bioprinting approach to spatiotemporally position different cellular layers may represent an efficient tool to develop living constructs especially for regeneration of complex flat tissues. |
| first_indexed | 2024-12-19T21:42:15Z |
| format | Article |
| id | doaj.art-c92de36ead404122ba83a4fd15662be8 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-19T21:42:15Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-c92de36ead404122ba83a4fd15662be82022-12-21T20:04:37ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-01-01710.3389/fbioe.2019.00478498317Direct-Write Bioprinting Approach to Construct Multilayer Cellular TissuesElahe Masaeli0Elahe Masaeli1Christophe Marquette2Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran3d.FAB, Univ Lyon, Université Lyon1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, Bat. Lederer, Villeurbanne, France3d.FAB, Univ Lyon, Université Lyon1, CNRS, INSA, CPE-Lyon, ICBMS, UMR 5246, Bat. Lederer, Villeurbanne, FranceAs a cellular-assembly technique, bioprinting has been extensively used in tissue engineering and regenerative medicine to construct hydrogel-based three-dimensional (3D) tissue-like models with prescribed geometry. Here, we introduced a unique direct-write bioprinting strategy to fabricate a bilayer flat tissue in a hydrogel-free approach. A printed retina pigmented epithelium layer (RPE) was applied as living biopaper for positioning a fibroblast layer without using any hydrogel in bioink. We adjusted the number of cells in the inkjet droplets in order to obtain a uniform printed cell layer and demonstrated the formation of a bilayer construct through confocal imaging. Since our printing system introduced low levels of shear stress to the cells, it did not have a negative effect on cell survival, although cell viability was generally lower than that of control group over 1 week post-printing. In conclusion, our novel direct-write bioprinting approach to spatiotemporally position different cellular layers may represent an efficient tool to develop living constructs especially for regeneration of complex flat tissues.https://www.frontiersin.org/article/10.3389/fbioe.2019.00478/fulltissue regenerationcell layerinkjet bioprintingliving biopapertissue complexity |
| spellingShingle | Elahe Masaeli Elahe Masaeli Christophe Marquette Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues Frontiers in Bioengineering and Biotechnology tissue regeneration cell layer inkjet bioprinting living biopaper tissue complexity |
| title | Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues |
| title_full | Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues |
| title_fullStr | Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues |
| title_full_unstemmed | Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues |
| title_short | Direct-Write Bioprinting Approach to Construct Multilayer Cellular Tissues |
| title_sort | direct write bioprinting approach to construct multilayer cellular tissues |
| topic | tissue regeneration cell layer inkjet bioprinting living biopaper tissue complexity |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2019.00478/full |
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