Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit
Brassinosteroids (BRs) play pivotal roles in improving plant stress tolerance. To investigate the mechanism of BR regulation of salt tolerance in kiwifruit, we used ‘Hongyang’ kiwifruit as the test material. We exposed the plants to 150 mmol/L NaCl stress and irrigated them with exogenous BR (2,4-ep...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/24/17252 |
| _version_ | 1797380790885023744 |
|---|---|
| author | Chen Chen Dawei Cheng Lan Li Xiaoxu Sun Shasha He Ming Li Jinyong Chen |
| author_facet | Chen Chen Dawei Cheng Lan Li Xiaoxu Sun Shasha He Ming Li Jinyong Chen |
| author_sort | Chen Chen |
| collection | DOAJ |
| description | Brassinosteroids (BRs) play pivotal roles in improving plant stress tolerance. To investigate the mechanism of BR regulation of salt tolerance in kiwifruit, we used ‘Hongyang’ kiwifruit as the test material. We exposed the plants to 150 mmol/L NaCl stress and irrigated them with exogenous BR (2,4-epibrassinolide). The phenotypic analysis showed that salt stress significantly inhibited photosynthesis in kiwifruit, leading to a significant increase in the H<sub>2</sub>O<sub>2</sub> content of leaves and roots and a significant increase in Na<sup>+</sup>/K<sup>+</sup>, resulting in oxidative damage and an ion imbalance. BR treatment resulted in enhanced photosynthesis, reduced H<sub>2</sub>O<sub>2</sub> content, and reduced Na<sup>+</sup>/K<sup>+</sup> in leaves, alleviating the salt stress injury. Furthermore, transcriptome enrichment analysis showed that the differentially expressed genes (DEGs) related to BR treatment are involved in pathways such as starch and sucrose metabolism, pentose and glucuronate interconversions, and plant hormone signal transduction, among others. Among the DEGs involved in plant hormone signal transduction, those with the highest expression were involved in abscisic acid signal transduction. Moreover, there was a significant increase in the expression of the <i>AcHKT1</i> gene, which regulates ion transduction, and the antioxidant enzyme <i>AcFSD2</i> gene, which is a key gene for improving salt tolerance. The data suggest that BRs can improve salt tolerance by regulating ion homeostasis and reducing oxidative stress. |
| first_indexed | 2024-03-08T20:42:09Z |
| format | Article |
| id | doaj.art-c92dfaf483da4342a76f7baa5b6f12d3 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:42:09Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-c92dfaf483da4342a76f7baa5b6f12d32023-12-22T14:13:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241725210.3390/ijms242417252Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated KiwifruitChen Chen0Dawei Cheng1Lan Li2Xiaoxu Sun3Shasha He4Ming Li5Jinyong Chen6National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, ChinaBrassinosteroids (BRs) play pivotal roles in improving plant stress tolerance. To investigate the mechanism of BR regulation of salt tolerance in kiwifruit, we used ‘Hongyang’ kiwifruit as the test material. We exposed the plants to 150 mmol/L NaCl stress and irrigated them with exogenous BR (2,4-epibrassinolide). The phenotypic analysis showed that salt stress significantly inhibited photosynthesis in kiwifruit, leading to a significant increase in the H<sub>2</sub>O<sub>2</sub> content of leaves and roots and a significant increase in Na<sup>+</sup>/K<sup>+</sup>, resulting in oxidative damage and an ion imbalance. BR treatment resulted in enhanced photosynthesis, reduced H<sub>2</sub>O<sub>2</sub> content, and reduced Na<sup>+</sup>/K<sup>+</sup> in leaves, alleviating the salt stress injury. Furthermore, transcriptome enrichment analysis showed that the differentially expressed genes (DEGs) related to BR treatment are involved in pathways such as starch and sucrose metabolism, pentose and glucuronate interconversions, and plant hormone signal transduction, among others. Among the DEGs involved in plant hormone signal transduction, those with the highest expression were involved in abscisic acid signal transduction. Moreover, there was a significant increase in the expression of the <i>AcHKT1</i> gene, which regulates ion transduction, and the antioxidant enzyme <i>AcFSD2</i> gene, which is a key gene for improving salt tolerance. The data suggest that BRs can improve salt tolerance by regulating ion homeostasis and reducing oxidative stress.https://www.mdpi.com/1422-0067/24/24/17252kiwifruitNaCl stress24-epibrassinolidephysiological conditionstranscriptome |
| spellingShingle | Chen Chen Dawei Cheng Lan Li Xiaoxu Sun Shasha He Ming Li Jinyong Chen Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit International Journal of Molecular Sciences kiwifruit NaCl stress 24-epibrassinolide physiological conditions transcriptome |
| title | Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit |
| title_full | Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit |
| title_fullStr | Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit |
| title_full_unstemmed | Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit |
| title_short | Physiological Characteristics and Transcriptome Analysis of Exogenous Brassinosteroid-Treated Kiwifruit |
| title_sort | physiological characteristics and transcriptome analysis of exogenous brassinosteroid treated kiwifruit |
| topic | kiwifruit NaCl stress 24-epibrassinolide physiological conditions transcriptome |
| url | https://www.mdpi.com/1422-0067/24/24/17252 |
| work_keys_str_mv | AT chenchen physiologicalcharacteristicsandtranscriptomeanalysisofexogenousbrassinosteroidtreatedkiwifruit AT daweicheng physiologicalcharacteristicsandtranscriptomeanalysisofexogenousbrassinosteroidtreatedkiwifruit AT lanli physiologicalcharacteristicsandtranscriptomeanalysisofexogenousbrassinosteroidtreatedkiwifruit AT xiaoxusun physiologicalcharacteristicsandtranscriptomeanalysisofexogenousbrassinosteroidtreatedkiwifruit AT shashahe physiologicalcharacteristicsandtranscriptomeanalysisofexogenousbrassinosteroidtreatedkiwifruit AT mingli physiologicalcharacteristicsandtranscriptomeanalysisofexogenousbrassinosteroidtreatedkiwifruit AT jinyongchen physiologicalcharacteristicsandtranscriptomeanalysisofexogenousbrassinosteroidtreatedkiwifruit |