Anti-microbial efficacy of Allium sativum extract against Enterococcus faecalis biofilm and its penetration into the root dentin: An in vitro study
Introduction: Sodium hypochlorite (NaOCl) has long been the most preferred root canal irrigant in endodontic treatment, but besides being an effective anti-microbial agent, it is highly cytotoxic. Thus, a search for an alternative herbal irrigant which would be more biocompatible but equally effecti...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2015-01-01
|
| Series: | Indian Journal of Dental Research |
| Subjects: | |
| Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2015;volume=26;issue=5;spage=477;epage=482;aulast=Birring |
| _version_ | 1818583621579046912 |
|---|---|
| author | Ourvind J.S. Birring Iluminada L Viloria Phides Nunez |
| author_facet | Ourvind J.S. Birring Iluminada L Viloria Phides Nunez |
| author_sort | Ourvind J.S. Birring |
| collection | DOAJ |
| description | Introduction: Sodium hypochlorite (NaOCl) has long been the most preferred root canal irrigant in endodontic treatment, but besides being an effective anti-microbial agent, it is highly cytotoxic. Thus, a search for an alternative herbal irrigant which would be more biocompatible but equally effective led to this study.
Aim: To assess the anti-microbial efficacy of garlic extract (GE) against Enterococcus faecalis biofilm and its ability to penetrate into root dentin.
Materials and Methods: E. faecalis was cultured and treated with the test agents - normal saline, 5.25% of NaOCl, and the three different concentrations of GE (10%, 40%, and 70%). The experiment was done in four groups namely, 24-h Co-treatment group, 24-h biofilm treatment group, 1-week biofilm group, and 3-week biofilm group. These groups were subjected to microbial viability assay and fluorescence microscopic analysis. The most effective concentration of garlic (70%) was further tested and compared with 5.25% NaOCl for its dentin penetration property using 0.2% alizarin red under a fluorescence microscope.
Results: The findings revealed that GE was able to disrupt as well as prevent the formation of biofilm produced by E. faecalis. All the concentrations of GE displayed considerable anti-microbial efficacy where 70% concentration was most effective and exhibited similar anti-microbial efficacy as 5.25% NaOCl. In terms of dentin penetration, no significant difference was found between GE and NaOCl.
Conclusion: The results indicate that GE has a potential to serve as an alternative herbal root canal irrigant being an effective and biocompatible anti-microbial agent with good dentinal penetration property. |
| first_indexed | 2024-12-16T08:08:12Z |
| format | Article |
| id | doaj.art-c93057652ef84e4884a4d5f4d20e5616 |
| institution | Directory Open Access Journal |
| issn | 0970-9290 1998-3603 |
| language | English |
| last_indexed | 2024-12-16T08:08:12Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj.art-c93057652ef84e4884a4d5f4d20e56162022-12-21T22:38:24ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032015-01-0126547748210.4103/0970-9290.172041Anti-microbial efficacy of Allium sativum extract against Enterococcus faecalis biofilm and its penetration into the root dentin: An in vitro studyOurvind J.S. BirringIluminada L ViloriaPhides NunezIntroduction: Sodium hypochlorite (NaOCl) has long been the most preferred root canal irrigant in endodontic treatment, but besides being an effective anti-microbial agent, it is highly cytotoxic. Thus, a search for an alternative herbal irrigant which would be more biocompatible but equally effective led to this study. Aim: To assess the anti-microbial efficacy of garlic extract (GE) against Enterococcus faecalis biofilm and its ability to penetrate into root dentin. Materials and Methods: E. faecalis was cultured and treated with the test agents - normal saline, 5.25% of NaOCl, and the three different concentrations of GE (10%, 40%, and 70%). The experiment was done in four groups namely, 24-h Co-treatment group, 24-h biofilm treatment group, 1-week biofilm group, and 3-week biofilm group. These groups were subjected to microbial viability assay and fluorescence microscopic analysis. The most effective concentration of garlic (70%) was further tested and compared with 5.25% NaOCl for its dentin penetration property using 0.2% alizarin red under a fluorescence microscope. Results: The findings revealed that GE was able to disrupt as well as prevent the formation of biofilm produced by E. faecalis. All the concentrations of GE displayed considerable anti-microbial efficacy where 70% concentration was most effective and exhibited similar anti-microbial efficacy as 5.25% NaOCl. In terms of dentin penetration, no significant difference was found between GE and NaOCl. Conclusion: The results indicate that GE has a potential to serve as an alternative herbal root canal irrigant being an effective and biocompatible anti-microbial agent with good dentinal penetration property.http://www.ijdr.in/article.asp?issn=0970-9290;year=2015;volume=26;issue=5;spage=477;epage=482;aulast=BirringBiofilmdentine penetrationEnterococcus faecalisfluorescence microscopygarlic extract |
| spellingShingle | Ourvind J.S. Birring Iluminada L Viloria Phides Nunez Anti-microbial efficacy of Allium sativum extract against Enterococcus faecalis biofilm and its penetration into the root dentin: An in vitro study Indian Journal of Dental Research Biofilm dentine penetration Enterococcus faecalis fluorescence microscopy garlic extract |
| title | Anti-microbial efficacy of Allium sativum extract against Enterococcus faecalis biofilm and its penetration into the root dentin: An in vitro study |
| title_full | Anti-microbial efficacy of Allium sativum extract against Enterococcus faecalis biofilm and its penetration into the root dentin: An in vitro study |
| title_fullStr | Anti-microbial efficacy of Allium sativum extract against Enterococcus faecalis biofilm and its penetration into the root dentin: An in vitro study |
| title_full_unstemmed | Anti-microbial efficacy of Allium sativum extract against Enterococcus faecalis biofilm and its penetration into the root dentin: An in vitro study |
| title_short | Anti-microbial efficacy of Allium sativum extract against Enterococcus faecalis biofilm and its penetration into the root dentin: An in vitro study |
| title_sort | anti microbial efficacy of allium sativum extract against enterococcus faecalis biofilm and its penetration into the root dentin an in vitro study |
| topic | Biofilm dentine penetration Enterococcus faecalis fluorescence microscopy garlic extract |
| url | http://www.ijdr.in/article.asp?issn=0970-9290;year=2015;volume=26;issue=5;spage=477;epage=482;aulast=Birring |
| work_keys_str_mv | AT ourvindjsbirring antimicrobialefficacyofalliumsativumextractagainstenterococcusfaecalisbiofilmanditspenetrationintotherootdentinaninvitrostudy AT iluminadalviloria antimicrobialefficacyofalliumsativumextractagainstenterococcusfaecalisbiofilmanditspenetrationintotherootdentinaninvitrostudy AT phidesnunez antimicrobialefficacyofalliumsativumextractagainstenterococcusfaecalisbiofilmanditspenetrationintotherootdentinaninvitrostudy |