Role of Claudins in Renal Branching Morphogenesis
Abstract Claudins are a family of tight junction proteins that are expressed during mouse kidney development. They regulate paracellular transport of solutes along the nephron and contribute to the final composition of the urinary filtrate. To understand their roles during development, we used a pro...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-09-01
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| Series: | Physiological Reports |
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| Online Access: | https://doi.org/10.14814/phy2.14492 |
| _version_ | 1829100829092610048 |
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| author | Jasmine El Andalousi Halim Khairallah Yuan Zhuang Aimee K. Ryan Indra R. Gupta |
| author_facet | Jasmine El Andalousi Halim Khairallah Yuan Zhuang Aimee K. Ryan Indra R. Gupta |
| author_sort | Jasmine El Andalousi |
| collection | DOAJ |
| description | Abstract Claudins are a family of tight junction proteins that are expressed during mouse kidney development. They regulate paracellular transport of solutes along the nephron and contribute to the final composition of the urinary filtrate. To understand their roles during development, we used a protein reagent, a truncated version of the Clostridium perfringens enterotoxin (C‐CPE), to specifically remove a subset of claudin family members from mouse embryonic kidney explants at embryonic day 12. We observed that treatment with C‐CPE decreased the number and the complexity of ureteric bud tips that formed: there were more single and less bifid ureteric bud tips when compared to control‐treated explants. In addition, C‐CPE‐treated explants exhibited ureteric bud tips with larger lumens when compared to control explants (p < .05). Immunofluorescent analysis revealed decreased expression and localization of Claudin‐3, −4, −6, and −8 to tight junctions of ureteric bud tips following treatment with C‐CPE. Interestingly, Claudin‐7 showed higher expression in the basolateral membrane of the ureteric bud lineage and poor localization to the tight junctions of the ureteric bud lineage both in controls and in C‐CPE‐treated explants. Taken together, it appears that claudin proteins may play a role in ureteric bud branching morphogenesis through changes in lumen formation that may affect the efficiency by which ureteric buds emerge and branch. |
| first_indexed | 2024-12-10T22:38:09Z |
| format | Article |
| id | doaj.art-c930c78e2a054f459b5bb9be72916bf3 |
| institution | Directory Open Access Journal |
| issn | 2051-817X |
| language | English |
| last_indexed | 2024-12-10T22:38:09Z |
| publishDate | 2020-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Physiological Reports |
| spelling | doaj.art-c930c78e2a054f459b5bb9be72916bf32022-12-22T01:30:48ZengWileyPhysiological Reports2051-817X2020-09-01818n/an/a10.14814/phy2.14492Role of Claudins in Renal Branching MorphogenesisJasmine El Andalousi0Halim Khairallah1Yuan Zhuang2Aimee K. Ryan3Indra R. Gupta4Research Institute of McGill University Health Centre Montreal Children's Hospital Montréal Québec CanadaDepartment of Human Genetics McGill University Montréal Québec CanadaDepartment of Human Genetics McGill University Montréal Québec CanadaDepartment of Human Genetics McGill University Montréal Québec CanadaDepartment of Human Genetics McGill University Montréal Québec CanadaAbstract Claudins are a family of tight junction proteins that are expressed during mouse kidney development. They regulate paracellular transport of solutes along the nephron and contribute to the final composition of the urinary filtrate. To understand their roles during development, we used a protein reagent, a truncated version of the Clostridium perfringens enterotoxin (C‐CPE), to specifically remove a subset of claudin family members from mouse embryonic kidney explants at embryonic day 12. We observed that treatment with C‐CPE decreased the number and the complexity of ureteric bud tips that formed: there were more single and less bifid ureteric bud tips when compared to control‐treated explants. In addition, C‐CPE‐treated explants exhibited ureteric bud tips with larger lumens when compared to control explants (p < .05). Immunofluorescent analysis revealed decreased expression and localization of Claudin‐3, −4, −6, and −8 to tight junctions of ureteric bud tips following treatment with C‐CPE. Interestingly, Claudin‐7 showed higher expression in the basolateral membrane of the ureteric bud lineage and poor localization to the tight junctions of the ureteric bud lineage both in controls and in C‐CPE‐treated explants. Taken together, it appears that claudin proteins may play a role in ureteric bud branching morphogenesis through changes in lumen formation that may affect the efficiency by which ureteric buds emerge and branch.https://doi.org/10.14814/phy2.14492kidney explantmousetight junction |
| spellingShingle | Jasmine El Andalousi Halim Khairallah Yuan Zhuang Aimee K. Ryan Indra R. Gupta Role of Claudins in Renal Branching Morphogenesis Physiological Reports kidney explant mouse tight junction |
| title | Role of Claudins in Renal Branching Morphogenesis |
| title_full | Role of Claudins in Renal Branching Morphogenesis |
| title_fullStr | Role of Claudins in Renal Branching Morphogenesis |
| title_full_unstemmed | Role of Claudins in Renal Branching Morphogenesis |
| title_short | Role of Claudins in Renal Branching Morphogenesis |
| title_sort | role of claudins in renal branching morphogenesis |
| topic | kidney explant mouse tight junction |
| url | https://doi.org/10.14814/phy2.14492 |
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