Evaluation of the In Vitro Antimicrobial Efficacy against <i>Staphylococcus aureus</i> and <i>epidermidis</i> of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study
Acute and chronic bone infections, especially those caused by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), remains a major complication and therapeutic challenge. It is documented that local administration of vancomycin offers better results than the usual routes of adminis...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/6/1763 |
| _version_ | 1797593074995560448 |
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| author | Iván López-González Ana Belén Hernández-Heredia María Isabel Rodríguez-López David Auñón-Calles Mohamed Boudifa José Antonio Gabaldón Luis Meseguer-Olmo |
| author_facet | Iván López-González Ana Belén Hernández-Heredia María Isabel Rodríguez-López David Auñón-Calles Mohamed Boudifa José Antonio Gabaldón Luis Meseguer-Olmo |
| author_sort | Iván López-González |
| collection | DOAJ |
| description | Acute and chronic bone infections, especially those caused by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), remains a major complication and therapeutic challenge. It is documented that local administration of vancomycin offers better results than the usual routes of administration (e.g., intravenous) when ischemic areas are present. In this work, we evaluate the antimicrobial efficacy against <i>S. aureus</i> and <i>S. epidermidis</i> of a novel hybrid 3D-printed scaffold based on polycaprolactone (PCL) and a chitosan (CS) hydrogel loaded with different vancomycin (Van) concentrations (1, 5, 10, 20%). Two cold plasma treatments were used to improve the adhesion of CS hydrogels to the PCL scaffolds by decreasing PCL hydrophobicity. Vancomycin release was measured by means of HPLC, and the biological response of <i>ah</i>-BM-MSCs growing in the presence of the scaffolds was evaluated in terms of cytotoxicity, proliferation, and osteogenic differentiation. The PCL/CS/Van scaffolds tested were found to be biocompatible, bioactive, and bactericide, as demonstrated by no cytotoxicity (LDH activity) or functional alteration (ALP activity, alizarin red staining) of the cultured cells and by bacterial inhibition. Our results suggest that the scaffolds developed would be excellent candidates for use in a wide range of biomedical fields such as drug delivery systems or tissue engineering applications. |
| first_indexed | 2024-03-11T02:02:32Z |
| format | Article |
| id | doaj.art-c9312fc93e5844b2acc24daf8126841a |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-11T02:02:32Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-c9312fc93e5844b2acc24daf8126841a2023-11-18T12:06:11ZengMDPI AGPharmaceutics1999-49232023-06-01156176310.3390/pharmaceutics15061763Evaluation of the In Vitro Antimicrobial Efficacy against <i>Staphylococcus aureus</i> and <i>epidermidis</i> of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical StudyIván López-González0Ana Belén Hernández-Heredia1María Isabel Rodríguez-López2David Auñón-Calles3Mohamed Boudifa4José Antonio Gabaldón5Luis Meseguer-Olmo6Tissue Regeneration and Repair Group: Orthobiology, Biomaterials and Tissue Engineering, UCAM—Universidad Católica de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107 Murcia, SpainMolecular Recognition and Encapsulation Research Group (REM), Health Sciences Department, UCAM—Universidad Católica de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107 Murcia, SpainMolecular Recognition and Encapsulation Research Group (REM), Health Sciences Department, UCAM—Universidad Católica de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107 Murcia, SpainMolecular Recognition and Encapsulation Research Group (REM), Health Sciences Department, UCAM—Universidad Católica de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107 Murcia, SpainCRITT—Matériaux Innovation, 9 Rue Claude Chrétien, Campus Sup Ardenne, 08000 Charleville-Mézières, FranceMolecular Recognition and Encapsulation Research Group (REM), Health Sciences Department, UCAM—Universidad Católica de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107 Murcia, SpainTissue Regeneration and Repair Group: Orthobiology, Biomaterials and Tissue Engineering, UCAM—Universidad Católica de Murcia, Campus de los Jerónimos 135, Guadalupe, 30107 Murcia, SpainAcute and chronic bone infections, especially those caused by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA), remains a major complication and therapeutic challenge. It is documented that local administration of vancomycin offers better results than the usual routes of administration (e.g., intravenous) when ischemic areas are present. In this work, we evaluate the antimicrobial efficacy against <i>S. aureus</i> and <i>S. epidermidis</i> of a novel hybrid 3D-printed scaffold based on polycaprolactone (PCL) and a chitosan (CS) hydrogel loaded with different vancomycin (Van) concentrations (1, 5, 10, 20%). Two cold plasma treatments were used to improve the adhesion of CS hydrogels to the PCL scaffolds by decreasing PCL hydrophobicity. Vancomycin release was measured by means of HPLC, and the biological response of <i>ah</i>-BM-MSCs growing in the presence of the scaffolds was evaluated in terms of cytotoxicity, proliferation, and osteogenic differentiation. The PCL/CS/Van scaffolds tested were found to be biocompatible, bioactive, and bactericide, as demonstrated by no cytotoxicity (LDH activity) or functional alteration (ALP activity, alizarin red staining) of the cultured cells and by bacterial inhibition. Our results suggest that the scaffolds developed would be excellent candidates for use in a wide range of biomedical fields such as drug delivery systems or tissue engineering applications.https://www.mdpi.com/1999-4923/15/6/17633D printinghybrid scaffoldpolycaprolactonechitosanvancomycinmesenchymal stem cells |
| spellingShingle | Iván López-González Ana Belén Hernández-Heredia María Isabel Rodríguez-López David Auñón-Calles Mohamed Boudifa José Antonio Gabaldón Luis Meseguer-Olmo Evaluation of the In Vitro Antimicrobial Efficacy against <i>Staphylococcus aureus</i> and <i>epidermidis</i> of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study Pharmaceutics 3D printing hybrid scaffold polycaprolactone chitosan vancomycin mesenchymal stem cells |
| title | Evaluation of the In Vitro Antimicrobial Efficacy against <i>Staphylococcus aureus</i> and <i>epidermidis</i> of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study |
| title_full | Evaluation of the In Vitro Antimicrobial Efficacy against <i>Staphylococcus aureus</i> and <i>epidermidis</i> of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study |
| title_fullStr | Evaluation of the In Vitro Antimicrobial Efficacy against <i>Staphylococcus aureus</i> and <i>epidermidis</i> of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study |
| title_full_unstemmed | Evaluation of the In Vitro Antimicrobial Efficacy against <i>Staphylococcus aureus</i> and <i>epidermidis</i> of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study |
| title_short | Evaluation of the In Vitro Antimicrobial Efficacy against <i>Staphylococcus aureus</i> and <i>epidermidis</i> of a Novel 3D-Printed Degradable Drug Delivery System Based on Polycaprolactone/Chitosan/Vancomycin—Preclinical Study |
| title_sort | evaluation of the in vitro antimicrobial efficacy against i staphylococcus aureus i and i epidermidis i of a novel 3d printed degradable drug delivery system based on polycaprolactone chitosan vancomycin preclinical study |
| topic | 3D printing hybrid scaffold polycaprolactone chitosan vancomycin mesenchymal stem cells |
| url | https://www.mdpi.com/1999-4923/15/6/1763 |
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