Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid Chromatography
A rapid and accurate HPLC method has been developed for the determination of pioglitazone hydrochloride in tablets. Chromatographic analysis was performed on a Nova-Pak® C18 column (3.9mm x 150mm, 5µm) with a mixture of ammonium formate buffer adjusted with formic acid to pH 3 and acetonitrile (75:2...
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| Format: | Article |
| Language: | English |
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National Centre of Excellence in Analytical Chemsitry
2008-12-01
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| Series: | Pakistan Journal of Analytical & Environmental Chemistry |
| Subjects: | |
| Online Access: | http://www.ceacsu.edu.pk/PDF%20file/Journal%20Volume%209,%20Issue%202/10.pdf |
| Summary: | A rapid and accurate HPLC method has been developed for the determination of pioglitazone hydrochloride in tablets. Chromatographic analysis was performed on a Nova-Pak® C18 column (3.9mm x 150mm, 5µm) with a mixture of ammonium formate buffer adjusted with formic acid to pH 3 and acetonitrile (75:25, v/v) as mobile phase, at flow rate of 1.0 mL min-1, and UV detection at 225 nm. The determination was completed in less than 12 min. Linearity ≥ 0.5 µgmL-1, accuracy ≥ 99.14 %, and precision ≤ 0.6 % were found to be acceptable over the range 0.5 - 20 µgmL-1. |
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| ISSN: | 1996-918X 2221-5255 |