Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid Chromatography
A rapid and accurate HPLC method has been developed for the determination of pioglitazone hydrochloride in tablets. Chromatographic analysis was performed on a Nova-Pak® C18 column (3.9mm x 150mm, 5µm) with a mixture of ammonium formate buffer adjusted with formic acid to pH 3 and acetonitrile (75:2...
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| Format: | Article |
| Language: | English |
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National Centre of Excellence in Analytical Chemsitry
2008-12-01
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| Series: | Pakistan Journal of Analytical & Environmental Chemistry |
| Subjects: | |
| Online Access: | http://www.ceacsu.edu.pk/PDF%20file/Journal%20Volume%209,%20Issue%202/10.pdf |
| _version_ | 1818528330258841600 |
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| author | AMR Lotfy Saber |
| author_facet | AMR Lotfy Saber |
| author_sort | AMR Lotfy Saber |
| collection | DOAJ |
| description | A rapid and accurate HPLC method has been developed for the determination of pioglitazone hydrochloride in tablets. Chromatographic analysis was performed on a Nova-Pak® C18 column (3.9mm x 150mm, 5µm) with a mixture of ammonium formate buffer adjusted with formic acid to pH 3 and acetonitrile (75:25, v/v) as mobile phase, at flow rate of 1.0 mL min-1, and UV detection at 225 nm. The determination was completed in less than 12 min. Linearity ≥ 0.5 µgmL-1, accuracy ≥ 99.14 %, and precision ≤ 0.6 % were found to be acceptable over the range 0.5 - 20 µgmL-1. |
| first_indexed | 2024-12-11T06:48:23Z |
| format | Article |
| id | doaj.art-c93174b28d7e4bfe9aa10ef860af3113 |
| institution | Directory Open Access Journal |
| issn | 1996-918X 2221-5255 |
| language | English |
| last_indexed | 2024-12-11T06:48:23Z |
| publishDate | 2008-12-01 |
| publisher | National Centre of Excellence in Analytical Chemsitry |
| record_format | Article |
| series | Pakistan Journal of Analytical & Environmental Chemistry |
| spelling | doaj.art-c93174b28d7e4bfe9aa10ef860af31132022-12-22T01:17:01ZengNational Centre of Excellence in Analytical ChemsitryPakistan Journal of Analytical & Environmental Chemistry1996-918X2221-52552008-12-0192118121Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid ChromatographyAMR Lotfy SaberA rapid and accurate HPLC method has been developed for the determination of pioglitazone hydrochloride in tablets. Chromatographic analysis was performed on a Nova-Pak® C18 column (3.9mm x 150mm, 5µm) with a mixture of ammonium formate buffer adjusted with formic acid to pH 3 and acetonitrile (75:25, v/v) as mobile phase, at flow rate of 1.0 mL min-1, and UV detection at 225 nm. The determination was completed in less than 12 min. Linearity ≥ 0.5 µgmL-1, accuracy ≥ 99.14 %, and precision ≤ 0.6 % were found to be acceptable over the range 0.5 - 20 µgmL-1.http://www.ceacsu.edu.pk/PDF%20file/Journal%20Volume%209,%20Issue%202/10.pdfPioglitazoneTabletsHPLC. |
| spellingShingle | AMR Lotfy Saber Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid Chromatography Pakistan Journal of Analytical & Environmental Chemistry Pioglitazone Tablets HPLC. |
| title | Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid Chromatography |
| title_full | Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid Chromatography |
| title_fullStr | Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid Chromatography |
| title_full_unstemmed | Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid Chromatography |
| title_short | Determination of Pioglitazone Hydrochloride in Tablets by High-Performance Liquid Chromatography |
| title_sort | determination of pioglitazone hydrochloride in tablets by high performance liquid chromatography |
| topic | Pioglitazone Tablets HPLC. |
| url | http://www.ceacsu.edu.pk/PDF%20file/Journal%20Volume%209,%20Issue%202/10.pdf |
| work_keys_str_mv | AT amrlotfysaber determinationofpioglitazonehydrochlorideintabletsbyhighperformanceliquidchromatography |