Characterization of Bacterial Exopolysaccharides Produced from Different Fruit-Based Solid Media
Bacterial exopolysaccharides (EPSs) share their compositional and structural features with plant dietary fibers. Therefore, analysis of bacterial EPSs produced during fermentation of fruit or vegetables interferes with that of plant fibers. To get rid of this effect, bacteria were grown on a solid m...
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MDPI AG
2023-07-01
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Series: | Fermentation |
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Online Access: | https://www.mdpi.com/2311-5637/9/7/657 |
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author | Marie Guérin Cyrielle Garcia Christine Robert-Da Silva Joël Couprie Fabienne Remize |
author_facet | Marie Guérin Cyrielle Garcia Christine Robert-Da Silva Joël Couprie Fabienne Remize |
author_sort | Marie Guérin |
collection | DOAJ |
description | Bacterial exopolysaccharides (EPSs) share their compositional and structural features with plant dietary fibers. Therefore, analysis of bacterial EPSs produced during fermentation of fruit or vegetables interferes with that of plant fibers. To get rid of this effect, bacteria were grown on a solid medium containing fruit juice or <i>purée</i> and EPSs were recovered in a quantitative dependent manner and were quantified with the phenol-sulfuric acid colorimetric method. The protocol was assayed both on MRS medium and fruit-based media, with three bacterial strains from two species, <i>Leuconostoc pseudomesenteroides</i> 56 and <i>Weissella cibaria</i> 21 and 64. With that method, differences in EPS production levels were shown according to the strain and cultivation conditions, such as sucrose content and pH. Complementary analysis with NMR indicated that glucose and sucrose were partly recovered with EPSs, pointing out the requirement for a further purification step. It also showed that EPSs’ ramified structure differed according to the strain and the fruit used in the medium. This method for EPS recovery is helpful to select strains and to pilot EPS production during lactic fermentation of fruit or vegetable foods. |
first_indexed | 2024-03-11T01:06:03Z |
format | Article |
id | doaj.art-c931a0e2f4d6404cb6fb17a94a136f9f |
institution | Directory Open Access Journal |
issn | 2311-5637 |
language | English |
last_indexed | 2024-03-11T01:06:03Z |
publishDate | 2023-07-01 |
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series | Fermentation |
spelling | doaj.art-c931a0e2f4d6404cb6fb17a94a136f9f2023-11-18T19:16:33ZengMDPI AGFermentation2311-56372023-07-019765710.3390/fermentation9070657Characterization of Bacterial Exopolysaccharides Produced from Different Fruit-Based Solid MediaMarie Guérin0Cyrielle Garcia1Christine Robert-Da Silva2Joël Couprie3Fabienne Remize4QualiSud, Université de La Réunion, CIRAD, Université Montpellier, Institut Agro Montpellier, Université Avignon, F-97410 Saint-Pierre, FranceQualiSud, Université de La Réunion, CIRAD, Université Montpellier, Institut Agro Montpellier, Université Avignon, F-97410 Saint-Pierre, FranceDiabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), Inserm, UMR 1188, Université de La Réunion, F-97490 Saint Denis, FranceDiabète Athérothrombose Thérapies Réunion Océan Indien (DéTROI), Inserm, UMR 1188, Université de La Réunion, F-97490 Saint Denis, FranceSPO, Université de Montpellier, Université de La Réunion, INRAE, Institut Agro Montpellier, F-34398 Montpellier, FranceBacterial exopolysaccharides (EPSs) share their compositional and structural features with plant dietary fibers. Therefore, analysis of bacterial EPSs produced during fermentation of fruit or vegetables interferes with that of plant fibers. To get rid of this effect, bacteria were grown on a solid medium containing fruit juice or <i>purée</i> and EPSs were recovered in a quantitative dependent manner and were quantified with the phenol-sulfuric acid colorimetric method. The protocol was assayed both on MRS medium and fruit-based media, with three bacterial strains from two species, <i>Leuconostoc pseudomesenteroides</i> 56 and <i>Weissella cibaria</i> 21 and 64. With that method, differences in EPS production levels were shown according to the strain and cultivation conditions, such as sucrose content and pH. Complementary analysis with NMR indicated that glucose and sucrose were partly recovered with EPSs, pointing out the requirement for a further purification step. It also showed that EPSs’ ramified structure differed according to the strain and the fruit used in the medium. This method for EPS recovery is helpful to select strains and to pilot EPS production during lactic fermentation of fruit or vegetable foods.https://www.mdpi.com/2311-5637/9/7/657pineapplemangopapaya<i>Weissella</i><i>Leuconostoc</i>homopolysaccharide |
spellingShingle | Marie Guérin Cyrielle Garcia Christine Robert-Da Silva Joël Couprie Fabienne Remize Characterization of Bacterial Exopolysaccharides Produced from Different Fruit-Based Solid Media Fermentation pineapple mango papaya <i>Weissella</i> <i>Leuconostoc</i> homopolysaccharide |
title | Characterization of Bacterial Exopolysaccharides Produced from Different Fruit-Based Solid Media |
title_full | Characterization of Bacterial Exopolysaccharides Produced from Different Fruit-Based Solid Media |
title_fullStr | Characterization of Bacterial Exopolysaccharides Produced from Different Fruit-Based Solid Media |
title_full_unstemmed | Characterization of Bacterial Exopolysaccharides Produced from Different Fruit-Based Solid Media |
title_short | Characterization of Bacterial Exopolysaccharides Produced from Different Fruit-Based Solid Media |
title_sort | characterization of bacterial exopolysaccharides produced from different fruit based solid media |
topic | pineapple mango papaya <i>Weissella</i> <i>Leuconostoc</i> homopolysaccharide |
url | https://www.mdpi.com/2311-5637/9/7/657 |
work_keys_str_mv | AT marieguerin characterizationofbacterialexopolysaccharidesproducedfromdifferentfruitbasedsolidmedia AT cyriellegarcia characterizationofbacterialexopolysaccharidesproducedfromdifferentfruitbasedsolidmedia AT christinerobertdasilva characterizationofbacterialexopolysaccharidesproducedfromdifferentfruitbasedsolidmedia AT joelcouprie characterizationofbacterialexopolysaccharidesproducedfromdifferentfruitbasedsolidmedia AT fabienneremize characterizationofbacterialexopolysaccharidesproducedfromdifferentfruitbasedsolidmedia |