Properties of Fluorescent Far-Red Anti-TNF Nanobodies
Upregulation of the expression of tumor necrosis factor (TNF-α, TNF) has a significant role in the development of autoimmune diseases. The fluorescent antibodies binding TNF may be used for personalized therapy of TNF-dependent diseases as a tool to predict the response to anti-TNF treatmen...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-12-01
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| Series: | Antibodies |
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| Online Access: | https://www.mdpi.com/2073-4468/7/4/43 |
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| author | Ekaterina N. Gorshkova Grigory A. Efimov Ksenia D. Ermakova Ekaterina A. Vasilenko Diana V. Yuzhakova Marina V. Shirmanova Vladislav V. Mokhonov Sergei V. Tillib Sergei A. Nedospasov Irina V. Astrakhantseva |
| author_facet | Ekaterina N. Gorshkova Grigory A. Efimov Ksenia D. Ermakova Ekaterina A. Vasilenko Diana V. Yuzhakova Marina V. Shirmanova Vladislav V. Mokhonov Sergei V. Tillib Sergei A. Nedospasov Irina V. Astrakhantseva |
| author_sort | Ekaterina N. Gorshkova |
| collection | DOAJ |
| description | Upregulation of the expression of tumor necrosis factor (TNF-α, TNF) has a significant role in the development of autoimmune diseases. The fluorescent antibodies binding TNF may be used for personalized therapy of TNF-dependent diseases as a tool to predict the response to anti-TNF treatment. We generated recombinant fluorescent proteins consisting of the anti-TNF module based on the variable heavy chain (VHH) of camelid antibodies fused with the far-red fluorescent protein Katushka (Kat). Two types of anti-TNF VHH were developed: one (BTN-Kat) that was bound both human or mouse TNF, but did not neutralize their activity, and a second (ITN-Kat) that was binding and neutralizing human TNF. BTN-Kat does not interfere with TNF biological functions and can be used for whole-body imaging. ITN-Kat can be evaluated in humanized mice or in cells isolated from humanized mice. It is able to block human TNF (hTNF) activities both in vitro and in vivo and may be considered as a prototype of a theranostic agent for autoimmune diseases. |
| first_indexed | 2024-12-11T19:36:39Z |
| format | Article |
| id | doaj.art-c932b57ac3ec4c239f2f52d04e923ce1 |
| institution | Directory Open Access Journal |
| issn | 2073-4468 |
| language | English |
| last_indexed | 2024-12-11T19:36:39Z |
| publishDate | 2018-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibodies |
| spelling | doaj.art-c932b57ac3ec4c239f2f52d04e923ce12022-12-22T00:53:09ZengMDPI AGAntibodies2073-44682018-12-01744310.3390/antib7040043antib7040043Properties of Fluorescent Far-Red Anti-TNF NanobodiesEkaterina N. Gorshkova0Grigory A. Efimov1Ksenia D. Ermakova2Ekaterina A. Vasilenko3Diana V. Yuzhakova4Marina V. Shirmanova5Vladislav V. Mokhonov6Sergei V. Tillib7Sergei A. Nedospasov8Irina V. Astrakhantseva9Center of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, Lobachevsky State University, Nizhniy Novgorod 603950, RussiaLaboratory of Transplantation Immunology, National Research Center for Hematology, Moscow 125167, RussiaCenter of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, Lobachevsky State University, Nizhniy Novgorod 603950, RussiaCenter of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, Lobachevsky State University, Nizhniy Novgorod 603950, RussiaInstitute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Nizhniy Novgorod 603005, RussiaInstitute of Biomedical Technologies, Nizhny Novgorod State Medical Academy, Nizhniy Novgorod 603005, RussiaCenter of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, Lobachevsky State University, Nizhniy Novgorod 603950, RussiaLomonosov Moscow State University, Moscow 119991, RussiaCenter of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, Lobachevsky State University, Nizhniy Novgorod 603950, RussiaCenter of Molecular Biology and Biomedicine, Institute of Biology and Biomedicine, Lobachevsky State University, Nizhniy Novgorod 603950, RussiaUpregulation of the expression of tumor necrosis factor (TNF-α, TNF) has a significant role in the development of autoimmune diseases. The fluorescent antibodies binding TNF may be used for personalized therapy of TNF-dependent diseases as a tool to predict the response to anti-TNF treatment. We generated recombinant fluorescent proteins consisting of the anti-TNF module based on the variable heavy chain (VHH) of camelid antibodies fused with the far-red fluorescent protein Katushka (Kat). Two types of anti-TNF VHH were developed: one (BTN-Kat) that was bound both human or mouse TNF, but did not neutralize their activity, and a second (ITN-Kat) that was binding and neutralizing human TNF. BTN-Kat does not interfere with TNF biological functions and can be used for whole-body imaging. ITN-Kat can be evaluated in humanized mice or in cells isolated from humanized mice. It is able to block human TNF (hTNF) activities both in vitro and in vivo and may be considered as a prototype of a theranostic agent for autoimmune diseases.https://www.mdpi.com/2073-4468/7/4/43TNFfluorescentnanobodiessensoranti-cytokine therapyautoimmune disease |
| spellingShingle | Ekaterina N. Gorshkova Grigory A. Efimov Ksenia D. Ermakova Ekaterina A. Vasilenko Diana V. Yuzhakova Marina V. Shirmanova Vladislav V. Mokhonov Sergei V. Tillib Sergei A. Nedospasov Irina V. Astrakhantseva Properties of Fluorescent Far-Red Anti-TNF Nanobodies Antibodies TNF fluorescent nanobodies sensor anti-cytokine therapy autoimmune disease |
| title | Properties of Fluorescent Far-Red Anti-TNF Nanobodies |
| title_full | Properties of Fluorescent Far-Red Anti-TNF Nanobodies |
| title_fullStr | Properties of Fluorescent Far-Red Anti-TNF Nanobodies |
| title_full_unstemmed | Properties of Fluorescent Far-Red Anti-TNF Nanobodies |
| title_short | Properties of Fluorescent Far-Red Anti-TNF Nanobodies |
| title_sort | properties of fluorescent far red anti tnf nanobodies |
| topic | TNF fluorescent nanobodies sensor anti-cytokine therapy autoimmune disease |
| url | https://www.mdpi.com/2073-4468/7/4/43 |
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