Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis Media
Lymphangiogenesis and angiogenesis might have significant involvement in the pathogenesis of otitis media with effusion. This study investigated the effect of diesel exhaust particles (DEP) on inflammation and lymphangiogenesis in a mouse model of acute otitis media (AOM). BALB/c mice were injected...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.824575/full |
| _version_ | 1818270976039714816 |
|---|---|
| author | Byeong-Gon Kim Byeong-Gon Kim Da Yeon Choi Min-Gyoung Kim An-Soo Jang Myung-Whan Suh Myung-Whan Suh Jun Ho Lee Jun Ho Lee Seung Ha Oh Seung Ha Oh Moo Kyun Park Moo Kyun Park |
| author_facet | Byeong-Gon Kim Byeong-Gon Kim Da Yeon Choi Min-Gyoung Kim An-Soo Jang Myung-Whan Suh Myung-Whan Suh Jun Ho Lee Jun Ho Lee Seung Ha Oh Seung Ha Oh Moo Kyun Park Moo Kyun Park |
| author_sort | Byeong-Gon Kim |
| collection | DOAJ |
| description | Lymphangiogenesis and angiogenesis might have significant involvement in the pathogenesis of otitis media with effusion. This study investigated the effect of diesel exhaust particles (DEP) on inflammation and lymphangiogenesis in a mouse model of acute otitis media (AOM). BALB/c mice were injected with LPS and exposed to 100 µg/m3 DEP. The mice were divided into four groups: control (no stimulation), AOM, AOM + DEP, and DEP + AOM.The effects of DEP inhalation pre- and post-DEP induction were estimated based on measurements of the auditory brainstem response, mRNA levels of lymphangiogenesis-related genes and cytokines, and histology of the middle ear. Cell viability of human middle ear epithelial cells decreased in a dose-response manner at 24 and 48 hours post-DEP exposure. DEP alone did not induce AOM. AOM-induced mice with pre- or post-DEP exposure showed thickened middle ear mucosa and increased expression of TNF-α and IL1-β mRNA levels compared to the control group, but increased serum IL-1β levels were not found in the AOM + Post DEP. The mRNA expression of TLR4, VEGFA, VEGFAC, and VEGFR3 was increased by pre-AOM DEP exposure.The expression of VEFGA protein was stronger in the AOM + Post DEP group than in any other group. The expression of CD31 and CD45 markers in the mouse middle ear tissue was higher in the Pre DEP + AOM group than in the AOM group. This result implies that pre-exposure to DEP more strongly increases inflammation and lymphangiogenesis in a mouse model of acute otitis media. |
| first_indexed | 2024-12-12T21:18:50Z |
| format | Article |
| id | doaj.art-c933c0a0f9d14edfa88e1c9fb0522b54 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-12T21:18:50Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-c933c0a0f9d14edfa88e1c9fb0522b542022-12-22T00:11:40ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-05-011210.3389/fcimb.2022.824575824575Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis MediaByeong-Gon Kim0Byeong-Gon Kim1Da Yeon Choi2Min-Gyoung Kim3An-Soo Jang4Myung-Whan Suh5Myung-Whan Suh6Jun Ho Lee7Jun Ho Lee8Seung Ha Oh9Seung Ha Oh10Moo Kyun Park11Moo Kyun Park12Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, South KoreaSensory Organ Research Institute, Seoul National University Medical Research Center, Seoul, South KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Soonchunhyang University Bucheon Hospital, Bucheon, South KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, South KoreaSensory Organ Research Institute, Seoul National University Medical Research Center, Seoul, South KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, South KoreaSensory Organ Research Institute, Seoul National University Medical Research Center, Seoul, South KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, South KoreaSensory Organ Research Institute, Seoul National University Medical Research Center, Seoul, South KoreaDepartment of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, South KoreaSensory Organ Research Institute, Seoul National University Medical Research Center, Seoul, South KoreaLymphangiogenesis and angiogenesis might have significant involvement in the pathogenesis of otitis media with effusion. This study investigated the effect of diesel exhaust particles (DEP) on inflammation and lymphangiogenesis in a mouse model of acute otitis media (AOM). BALB/c mice were injected with LPS and exposed to 100 µg/m3 DEP. The mice were divided into four groups: control (no stimulation), AOM, AOM + DEP, and DEP + AOM.The effects of DEP inhalation pre- and post-DEP induction were estimated based on measurements of the auditory brainstem response, mRNA levels of lymphangiogenesis-related genes and cytokines, and histology of the middle ear. Cell viability of human middle ear epithelial cells decreased in a dose-response manner at 24 and 48 hours post-DEP exposure. DEP alone did not induce AOM. AOM-induced mice with pre- or post-DEP exposure showed thickened middle ear mucosa and increased expression of TNF-α and IL1-β mRNA levels compared to the control group, but increased serum IL-1β levels were not found in the AOM + Post DEP. The mRNA expression of TLR4, VEGFA, VEGFAC, and VEGFR3 was increased by pre-AOM DEP exposure.The expression of VEFGA protein was stronger in the AOM + Post DEP group than in any other group. The expression of CD31 and CD45 markers in the mouse middle ear tissue was higher in the Pre DEP + AOM group than in the AOM group. This result implies that pre-exposure to DEP more strongly increases inflammation and lymphangiogenesis in a mouse model of acute otitis media.https://www.frontiersin.org/articles/10.3389/fcimb.2022.824575/fulldiesel exhaust particleslipopolysaccharidesotitis medialymphangiogenesisangiogenesis |
| spellingShingle | Byeong-Gon Kim Byeong-Gon Kim Da Yeon Choi Min-Gyoung Kim An-Soo Jang Myung-Whan Suh Myung-Whan Suh Jun Ho Lee Jun Ho Lee Seung Ha Oh Seung Ha Oh Moo Kyun Park Moo Kyun Park Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis Media Frontiers in Cellular and Infection Microbiology diesel exhaust particles lipopolysaccharides otitis media lymphangiogenesis angiogenesis |
| title | Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis Media |
| title_full | Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis Media |
| title_fullStr | Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis Media |
| title_full_unstemmed | Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis Media |
| title_short | Effect of Angiogenesis and Lymphangiogenesis in Diesel Exhaust Particles Inhalation in Mouse Model of LPS Induced Acute Otitis Media |
| title_sort | effect of angiogenesis and lymphangiogenesis in diesel exhaust particles inhalation in mouse model of lps induced acute otitis media |
| topic | diesel exhaust particles lipopolysaccharides otitis media lymphangiogenesis angiogenesis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.824575/full |
| work_keys_str_mv | AT byeonggonkim effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT byeonggonkim effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT dayeonchoi effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT mingyoungkim effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT ansoojang effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT myungwhansuh effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT myungwhansuh effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT junholee effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT junholee effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT seunghaoh effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT seunghaoh effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT mookyunpark effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia AT mookyunpark effectofangiogenesisandlymphangiogenesisindieselexhaustparticlesinhalationinmousemodeloflpsinducedacuteotitismedia |