CRISPR/Cas9-gene editing approaches in plant breeding
ABSTRACTCRISPR/Cas9 gene editing system is recently developed robust genome editing technology for accelerating plant breeding. Various modifications of this editing system have been established for adaptability in plant varieties as well as for its improved efficiency and portability. This review p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | GM Crops & Food |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21645698.2023.2256930 |
| _version_ | 1827399042601058304 |
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| author | Himanshu Saini Rajneesh Thakur Rubina Gill Kalpana Tyagi Manika Goswami |
| author_facet | Himanshu Saini Rajneesh Thakur Rubina Gill Kalpana Tyagi Manika Goswami |
| author_sort | Himanshu Saini |
| collection | DOAJ |
| description | ABSTRACTCRISPR/Cas9 gene editing system is recently developed robust genome editing technology for accelerating plant breeding. Various modifications of this editing system have been established for adaptability in plant varieties as well as for its improved efficiency and portability. This review provides an in-depth look at the various strategies for synthesizing gRNAs for efficient delivery in plant cells, including chemical synthesis and in vitro transcription. It also covers traditional analytical tools and emerging developments in detection methods to analyze CRISPR/Cas9 mediated mutation in plant breeding. Additionally, the review outlines the various analytical tools which are used to detect and analyze CRISPR/Cas9 mediated mutations, such as next-generation sequencing, restriction enzyme analysis, and southern blotting. Finally, the review discusses emerging detection methods, including digital PCR and qPCR. Hence, CRISPR/Cas9 has great potential for transforming agriculture and opening avenues for new advancements in the system for gene editing in plants. |
| first_indexed | 2024-03-08T19:39:47Z |
| format | Article |
| id | doaj.art-c9347173a5194bb2b26591db7bbafb9e |
| institution | Directory Open Access Journal |
| issn | 2164-5698 2164-5701 |
| language | English |
| last_indexed | 2024-03-08T19:39:47Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | GM Crops & Food |
| spelling | doaj.art-c9347173a5194bb2b26591db7bbafb9e2023-12-25T13:28:18ZengTaylor & Francis GroupGM Crops & Food2164-56982164-57012023-12-0114111710.1080/21645698.2023.2256930CRISPR/Cas9-gene editing approaches in plant breedingHimanshu Saini0Rajneesh Thakur1Rubina Gill2Kalpana Tyagi3Manika Goswami4School of Applied Natural Science, Adama Science and Technology University, Adama, EthiopiaDepartment of Plant Pathology, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, IndiaDepartment of Agronomy, School of Agriculture, Lovely professional university, Phagwara, Punjab, IndiaDivision of Genetics and Tree Improvement, Forest Research Institute, Dehradun, Uttarakhand, IndiaDepartment of Fruit Science, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, IndiaABSTRACTCRISPR/Cas9 gene editing system is recently developed robust genome editing technology for accelerating plant breeding. Various modifications of this editing system have been established for adaptability in plant varieties as well as for its improved efficiency and portability. This review provides an in-depth look at the various strategies for synthesizing gRNAs for efficient delivery in plant cells, including chemical synthesis and in vitro transcription. It also covers traditional analytical tools and emerging developments in detection methods to analyze CRISPR/Cas9 mediated mutation in plant breeding. Additionally, the review outlines the various analytical tools which are used to detect and analyze CRISPR/Cas9 mediated mutations, such as next-generation sequencing, restriction enzyme analysis, and southern blotting. Finally, the review discusses emerging detection methods, including digital PCR and qPCR. Hence, CRISPR/Cas9 has great potential for transforming agriculture and opening avenues for new advancements in the system for gene editing in plants.https://www.tandfonline.com/doi/10.1080/21645698.2023.2256930AgricultureCrispr/Cas9gene editinggenomeplant breeding |
| spellingShingle | Himanshu Saini Rajneesh Thakur Rubina Gill Kalpana Tyagi Manika Goswami CRISPR/Cas9-gene editing approaches in plant breeding GM Crops & Food Agriculture Crispr/Cas9 gene editing genome plant breeding |
| title | CRISPR/Cas9-gene editing approaches in plant breeding |
| title_full | CRISPR/Cas9-gene editing approaches in plant breeding |
| title_fullStr | CRISPR/Cas9-gene editing approaches in plant breeding |
| title_full_unstemmed | CRISPR/Cas9-gene editing approaches in plant breeding |
| title_short | CRISPR/Cas9-gene editing approaches in plant breeding |
| title_sort | crispr cas9 gene editing approaches in plant breeding |
| topic | Agriculture Crispr/Cas9 gene editing genome plant breeding |
| url | https://www.tandfonline.com/doi/10.1080/21645698.2023.2256930 |
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