The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly
Early steps of large 60S ribosomal subunit biogenesis are not well understood. Here, the authors combine biochemical experiments with protein-RNA crosslinking and mass spectrometry to show that the RNA helicase Dbp7 is key player during early 60S ribosomal assembly. Dbp7 regulates a series of events...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-10-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-26208-9 |
| _version_ | 1818575900133818368 |
|---|---|
| author | Gerald Ryan R. Aquino Philipp Hackert Nicolai Krogh Kuan-Ting Pan Mariam Jaafar Anthony K. Henras Henrik Nielsen Henning Urlaub Katherine E. Bohnsack Markus T. Bohnsack |
| author_facet | Gerald Ryan R. Aquino Philipp Hackert Nicolai Krogh Kuan-Ting Pan Mariam Jaafar Anthony K. Henras Henrik Nielsen Henning Urlaub Katherine E. Bohnsack Markus T. Bohnsack |
| author_sort | Gerald Ryan R. Aquino |
| collection | DOAJ |
| description | Early steps of large 60S ribosomal subunit biogenesis are not well understood. Here, the authors combine biochemical experiments with protein-RNA crosslinking and mass spectrometry to show that the RNA helicase Dbp7 is key player during early 60S ribosomal assembly. Dbp7 regulates a series of events driving compaction of domain V/VI in early pre60S ribosomal particles. |
| first_indexed | 2024-12-15T00:47:23Z |
| format | Article |
| id | doaj.art-c9348e45ad3e4a0888725af478868ba9 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-15T00:47:23Z |
| publishDate | 2021-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-c9348e45ad3e4a0888725af478868ba92022-12-21T22:41:31ZengNature PortfolioNature Communications2041-17232021-10-0112111610.1038/s41467-021-26208-9The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assemblyGerald Ryan R. Aquino0Philipp Hackert1Nicolai Krogh2Kuan-Ting Pan3Mariam Jaafar4Anthony K. Henras5Henrik Nielsen6Henning Urlaub7Katherine E. Bohnsack8Markus T. Bohnsack9Department of Molecular Biology, University Medical Center GöttingenDepartment of Molecular Biology, University Medical Center GöttingenDepartment of Cellular and Molecular Medicine, University of CopenhagenMax Planck Institute for Biophysical Chemistry, Bioanalytical Mass SpectrometryMolecular, Cellular and Developmental Biology Unit (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPSMolecular, Cellular and Developmental Biology Unit (MCD), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPSDepartment of Cellular and Molecular Medicine, University of CopenhagenMax Planck Institute for Biophysical Chemistry, Bioanalytical Mass SpectrometryDepartment of Molecular Biology, University Medical Center GöttingenDepartment of Molecular Biology, University Medical Center GöttingenEarly steps of large 60S ribosomal subunit biogenesis are not well understood. Here, the authors combine biochemical experiments with protein-RNA crosslinking and mass spectrometry to show that the RNA helicase Dbp7 is key player during early 60S ribosomal assembly. Dbp7 regulates a series of events driving compaction of domain V/VI in early pre60S ribosomal particles.https://doi.org/10.1038/s41467-021-26208-9 |
| spellingShingle | Gerald Ryan R. Aquino Philipp Hackert Nicolai Krogh Kuan-Ting Pan Mariam Jaafar Anthony K. Henras Henrik Nielsen Henning Urlaub Katherine E. Bohnsack Markus T. Bohnsack The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly Nature Communications |
| title | The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly |
| title_full | The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly |
| title_fullStr | The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly |
| title_full_unstemmed | The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly |
| title_short | The RNA helicase Dbp7 promotes domain V/VI compaction and stabilization of inter-domain interactions during early 60S assembly |
| title_sort | rna helicase dbp7 promotes domain v vi compaction and stabilization of inter domain interactions during early 60s assembly |
| url | https://doi.org/10.1038/s41467-021-26208-9 |
| work_keys_str_mv | AT geraldryanraquino thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT philipphackert thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT nicolaikrogh thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT kuantingpan thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT mariamjaafar thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT anthonykhenras thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT henriknielsen thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT henningurlaub thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT katherineebohnsack thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT markustbohnsack thernahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT geraldryanraquino rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT philipphackert rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT nicolaikrogh rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT kuantingpan rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT mariamjaafar rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT anthonykhenras rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT henriknielsen rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT henningurlaub rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT katherineebohnsack rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly AT markustbohnsack rnahelicasedbp7promotesdomainvvicompactionandstabilizationofinterdomaininteractionsduringearly60sassembly |