Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing.
Composting is viewed as one of the primary methods to treat organic wastes. Co-composting may improve the efficiency of this treatment by establishing the most suitable conditions for decomposers than those present in the individual wastes. Given that bacteria and fungi are the driving agents of com...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5653195?pdf=render |
| _version_ | 1818965390628225024 |
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| author | Polina Galitskaya Liliya Biktasheva Anatoly Saveliev Tatiana Grigoryeva Eugenia Boulygina Svetlana Selivanovskaya |
| author_facet | Polina Galitskaya Liliya Biktasheva Anatoly Saveliev Tatiana Grigoryeva Eugenia Boulygina Svetlana Selivanovskaya |
| author_sort | Polina Galitskaya |
| collection | DOAJ |
| description | Composting is viewed as one of the primary methods to treat organic wastes. Co-composting may improve the efficiency of this treatment by establishing the most suitable conditions for decomposers than those present in the individual wastes. Given that bacteria and fungi are the driving agents of composting, information about the composition of their communities and dynamics during composting may improve reproducibility, performance and quality of the final compost as well as help to evaluate the potential human health risk and the choice of the most appropriate application procedure. In this study, the co-composting of mixtures containing two similar components (organic fraction of municipal solid waste and sawdust polluted by oil) and one discriminate component (sewage sludges of different origin) were investigated. Bacterial and fungal community successions in the two mixtures were analyzed during the composting process by determining the change in their structural dynamics using qPCR and 454 pyrosequencing methods in a lab experiment for a period of 270 days. During the initial composting stage, the number of 16S bacterial copies was (3.0±0.2) x 106 and (0.4±0.0) x 107 g-1, and the Rhodospiralles and Lactobacialles orders dominated. Fungal communities had (2.9±0.0) x105 and (6.1±0.2) x105 ITS copies g-1, and the Saccharomycetales order dominated. At the end of the thermophilic stage on the 30th day of composting, bacterial and fungal communities underwent significant changes: dominants changed and their relative abundance decreased. Typical compost residents included Flavobacteriales, Chitinophagaceae and Bacterioidetes for bacteria and Microascaceae, Dothideomycetes, Eurotiomycetes, Sordariomycetes, and Agaricomycetes for fungi. During the later composting stages, the dominating taxa of both bacterial and fungal communities remained, while their relative abundance decreased. In accordance with the change in the dominating OTUs, it was concluded that the dynamics of the bacterial and fungal communities were not similar. Analysis by non-metric multidimensional scaling (NMDS) revealed that the bacterial communities of the two composts became progressively more similar; a similar trend was followed by the fungal community. |
| first_indexed | 2024-12-20T13:16:15Z |
| format | Article |
| id | doaj.art-c93497b78c7e42098e4c938bba405304 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T13:16:15Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c93497b78c7e42098e4c938bba4053042022-12-21T19:39:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011210e018605110.1371/journal.pone.0186051Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing.Polina GalitskayaLiliya BiktashevaAnatoly SavelievTatiana GrigoryevaEugenia BoulyginaSvetlana SelivanovskayaComposting is viewed as one of the primary methods to treat organic wastes. Co-composting may improve the efficiency of this treatment by establishing the most suitable conditions for decomposers than those present in the individual wastes. Given that bacteria and fungi are the driving agents of composting, information about the composition of their communities and dynamics during composting may improve reproducibility, performance and quality of the final compost as well as help to evaluate the potential human health risk and the choice of the most appropriate application procedure. In this study, the co-composting of mixtures containing two similar components (organic fraction of municipal solid waste and sawdust polluted by oil) and one discriminate component (sewage sludges of different origin) were investigated. Bacterial and fungal community successions in the two mixtures were analyzed during the composting process by determining the change in their structural dynamics using qPCR and 454 pyrosequencing methods in a lab experiment for a period of 270 days. During the initial composting stage, the number of 16S bacterial copies was (3.0±0.2) x 106 and (0.4±0.0) x 107 g-1, and the Rhodospiralles and Lactobacialles orders dominated. Fungal communities had (2.9±0.0) x105 and (6.1±0.2) x105 ITS copies g-1, and the Saccharomycetales order dominated. At the end of the thermophilic stage on the 30th day of composting, bacterial and fungal communities underwent significant changes: dominants changed and their relative abundance decreased. Typical compost residents included Flavobacteriales, Chitinophagaceae and Bacterioidetes for bacteria and Microascaceae, Dothideomycetes, Eurotiomycetes, Sordariomycetes, and Agaricomycetes for fungi. During the later composting stages, the dominating taxa of both bacterial and fungal communities remained, while their relative abundance decreased. In accordance with the change in the dominating OTUs, it was concluded that the dynamics of the bacterial and fungal communities were not similar. Analysis by non-metric multidimensional scaling (NMDS) revealed that the bacterial communities of the two composts became progressively more similar; a similar trend was followed by the fungal community.http://europepmc.org/articles/PMC5653195?pdf=render |
| spellingShingle | Polina Galitskaya Liliya Biktasheva Anatoly Saveliev Tatiana Grigoryeva Eugenia Boulygina Svetlana Selivanovskaya Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing. PLoS ONE |
| title | Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing. |
| title_full | Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing. |
| title_fullStr | Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing. |
| title_full_unstemmed | Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing. |
| title_short | Fungal and bacterial successions in the process of co-composting of organic wastes as revealed by 454 pyrosequencing. |
| title_sort | fungal and bacterial successions in the process of co composting of organic wastes as revealed by 454 pyrosequencing |
| url | http://europepmc.org/articles/PMC5653195?pdf=render |
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