Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringin
ABSTRACT Background and Objectives: Citrus fruits and their constituents especially naringin (NR), a natural predominant flavanone, have a wide range of pharmacological activities without toxicity against cancer cells. The aim of this study was to investigate the anticancer effects of or...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2020-03-01
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| Series: | Medical Laboratory Journal |
| Subjects: | |
| Online Access: | http://mlj.goums.ac.ir/article-1-1238-en.html |
| _version_ | 1828773105705680896 |
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| author | mahboubeh tajaldini Firooz Samadi Ayyoob Khosravi Azim Ghasemnejad Jahanbakhsh Asadi |
| author_facet | mahboubeh tajaldini Firooz Samadi Ayyoob Khosravi Azim Ghasemnejad Jahanbakhsh Asadi |
| author_sort | mahboubeh tajaldini |
| collection | DOAJ |
| description | ABSTRACT
Background and Objectives: Citrus fruits and their constituents especially naringin (NR), a natural predominant flavanone, have a wide range of pharmacological activities without toxicity against cancer cells. The aim of this study was to investigate the anticancer effects of orange peel extract (OPE) and naringin (NR) on esophageal squamous cell carcinoma (ESCC) cells.
Methods: Amount of phenol, flavonoid and antioxidants in OPE was determined using Folin-Ciocalteu procedure, aluminum chloride colorimetric and DPPH assays, respectively. Effects of NR and OPE on viability, wound healing assay and DNA fragmentation using DAPI were investigated. Data were analyzed by ImageJ software and GraphPad Prism 6.0 at significance of 0.05.
Results: Total amount of phenols, flavonoids and 1,1-diphenyl-2-picrylhydrazyl was 2.83, 2.143 and 60.76 g/100g of OPE. Amount of NR in the dried OPE was estimated to be 5.260 (µg/gr) using high-performance liquid chromatography. Treatment of ESCC cells with OPE or NR decreased viability y of cancer cells in a dose-dependent manner. In addition, both OPE and NR were able to decrease cell migration and increase DNA fragmentation.
Conclusion: The findings of our study suggest that OPE and NR have anticancer effects on ESCC cells but the anticancer effects of OPE was better than that of NR alone.
Keywords: Orange peel extract, Naringin, Migration, Esophageal squamous cell carcinoma. |
| first_indexed | 2024-12-11T14:57:05Z |
| format | Article |
| id | doaj.art-c9358f49537e48cbb2c169ee73e6f63f |
| institution | Directory Open Access Journal |
| issn | 2538-4449 |
| language | English |
| last_indexed | 2024-12-11T14:57:05Z |
| publishDate | 2020-03-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Medical Laboratory Journal |
| spelling | doaj.art-c9358f49537e48cbb2c169ee73e6f63f2022-12-22T01:01:17ZengGolestan University of Medical SciencesMedical Laboratory Journal2538-44492020-03-011423135Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringinmahboubeh tajaldini0Firooz Samadi1Ayyoob Khosravi2Azim Ghasemnejad3Jahanbakhsh Asadi4 Department of Animal and Poultry Physiology, Faculty of Animal Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Department of Animal and Poultry Physiology, Faculty of Animal Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran Department of Molecular Medicine, Faculty of Medical Sciences, Golestan University of Medical Sciences, Gorgan, Iran Department of horticultural Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Metabolic Disorder Center, Golestan University of Medical Sciences, Gorgan, Iran ABSTRACT Background and Objectives: Citrus fruits and their constituents especially naringin (NR), a natural predominant flavanone, have a wide range of pharmacological activities without toxicity against cancer cells. The aim of this study was to investigate the anticancer effects of orange peel extract (OPE) and naringin (NR) on esophageal squamous cell carcinoma (ESCC) cells. Methods: Amount of phenol, flavonoid and antioxidants in OPE was determined using Folin-Ciocalteu procedure, aluminum chloride colorimetric and DPPH assays, respectively. Effects of NR and OPE on viability, wound healing assay and DNA fragmentation using DAPI were investigated. Data were analyzed by ImageJ software and GraphPad Prism 6.0 at significance of 0.05. Results: Total amount of phenols, flavonoids and 1,1-diphenyl-2-picrylhydrazyl was 2.83, 2.143 and 60.76 g/100g of OPE. Amount of NR in the dried OPE was estimated to be 5.260 (µg/gr) using high-performance liquid chromatography. Treatment of ESCC cells with OPE or NR decreased viability y of cancer cells in a dose-dependent manner. In addition, both OPE and NR were able to decrease cell migration and increase DNA fragmentation. Conclusion: The findings of our study suggest that OPE and NR have anticancer effects on ESCC cells but the anticancer effects of OPE was better than that of NR alone. Keywords: Orange peel extract, Naringin, Migration, Esophageal squamous cell carcinoma.http://mlj.goums.ac.ir/article-1-1238-en.htmlorange peel extractnaringinmigrationesophageal squamous cell carcinoma. |
| spellingShingle | mahboubeh tajaldini Firooz Samadi Ayyoob Khosravi Azim Ghasemnejad Jahanbakhsh Asadi Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringin Medical Laboratory Journal orange peel extract naringin migration esophageal squamous cell carcinoma. |
| title | Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringin |
| title_full | Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringin |
| title_fullStr | Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringin |
| title_full_unstemmed | Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringin |
| title_short | Inhibition of Growth and Migration of Esophageal Squamous Cell Carcinoma Cells by Orange Peel Extract and Naringin |
| title_sort | inhibition of growth and migration of esophageal squamous cell carcinoma cells by orange peel extract and naringin |
| topic | orange peel extract naringin migration esophageal squamous cell carcinoma. |
| url | http://mlj.goums.ac.ir/article-1-1238-en.html |
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