Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut
The aim of this study was to evaluate antimicrobial activity of Lactobacillus kunkeei of bee’s gastrointestinal tract (GUT) against pathogenic bacteria isolated from bees’ gut. Content of the intestinal tract microbiota was cultured for isolation of different group of bacteria. Gut homogenates were...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-09-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
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| Online Access: | https://spasb.ro/index.php/public_html/article/view/869 |
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| author | Miroslava Kačániová Simona Kunová Eva Ivanišová Margarita Terentjeva Jaroslav Gasper |
| author_facet | Miroslava Kačániová Simona Kunová Eva Ivanišová Margarita Terentjeva Jaroslav Gasper |
| author_sort | Miroslava Kačániová |
| collection | DOAJ |
| description | The aim of this study was to evaluate antimicrobial activity of Lactobacillus kunkeei of bee’s gastrointestinal tract (GUT) against pathogenic bacteria isolated from bees’ gut. Content of the intestinal tract microbiota was cultured for isolation of different group of bacteria. Gut homogenates were plated on de Man, Rogosa and Sharpe agar plates and incubated for 48-72h at 30 °C anaerobically; on McConkey agar and incubated for 24-48h at 37°C aerobically. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. The bacterial strains of Lactobacillus kunkeei, Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis, Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens and Sphingomonas parapaucimobilis were isolated from gut content of bees. The disc diffusion method was used for the determination of antimicrobial activities of the Lactobacillus kunkeei supernatant against other isolated bacterial strains of bees’ origin. The best antimicrobial activity of Lactobacillus kunkeei supernatant was found against Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens. Less effective the L. kunkeei supernatant was against Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis. The moderate antibacterial activity was identified against Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Sphingomonas parapaucimobilis. |
| first_indexed | 2024-04-24T11:23:55Z |
| format | Article |
| id | doaj.art-c939c37cc2464d0fbe1ad42b18908b94 |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:23:55Z |
| publishDate | 2023-09-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-c939c37cc2464d0fbe1ad42b18908b942024-04-11T03:52:11ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-09-01522100100869Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ GutMiroslava Kačániová0Simona Kunová1Eva Ivanišová2Margarita Terentjeva3Jaroslav Gasper4Slovak University of Agriculture, Faculty of Horticulture and Landscape Engineering, Nitra 949 76, Tr. A. Hlinku 2, Slovakia Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, K. Helmaņa iela 8, LV-3004, Jelgava, LatviaSlovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia The aim of this study was to evaluate antimicrobial activity of Lactobacillus kunkeei of bee’s gastrointestinal tract (GUT) against pathogenic bacteria isolated from bees’ gut. Content of the intestinal tract microbiota was cultured for isolation of different group of bacteria. Gut homogenates were plated on de Man, Rogosa and Sharpe agar plates and incubated for 48-72h at 30 °C anaerobically; on McConkey agar and incubated for 24-48h at 37°C aerobically. Then, the identification of isolates with MALDI-TOF MS Biotyper was done. The bacterial strains of Lactobacillus kunkeei, Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis, Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens and Sphingomonas parapaucimobilis were isolated from gut content of bees. The disc diffusion method was used for the determination of antimicrobial activities of the Lactobacillus kunkeei supernatant against other isolated bacterial strains of bees’ origin. The best antimicrobial activity of Lactobacillus kunkeei supernatant was found against Klebsiella aerogenes, Morganella morganii, Pseudomonas aeruginosa, Serratia marcescens. Less effective the L. kunkeei supernatant was against Bacillus cereus, Bacillus megatherium, Bacillus simplex, Bacillus thuringiensis. The moderate antibacterial activity was identified against Delfia acidovorans, Escherichia coli, Enterococcus faecalis, Sphingomonas parapaucimobilis.https://spasb.ro/index.php/public_html/article/view/869lactobacillus kunkeeiantimicrobial activitybeespathogenic bacteria |
| spellingShingle | Miroslava Kačániová Simona Kunová Eva Ivanišová Margarita Terentjeva Jaroslav Gasper Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut Scientific Papers Animal Science and Biotechnologies lactobacillus kunkeei antimicrobial activity bees pathogenic bacteria |
| title | Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut |
| title_full | Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut |
| title_fullStr | Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut |
| title_full_unstemmed | Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut |
| title_short | Antimicrobial Effect of Lactobacillus kunkeei Against Pathogenic Bacteria Isolated from Bees’ Gut |
| title_sort | antimicrobial effect of lactobacillus kunkeei against pathogenic bacteria isolated from bees gut |
| topic | lactobacillus kunkeei antimicrobial activity bees pathogenic bacteria |
| url | https://spasb.ro/index.php/public_html/article/view/869 |
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