Isolation and characterization of mitochondrial DNA from the endangered white-clawed crayfish Austropotamobius pallipes pallipes, Lereboullet, 1858
Mitochondrial DNA (mtDNA) variation in the white-clawed crayfish, Austropotamobius pallipes pallipes, was examined by restriction endonuclease analysis of samples obtained from three geographical locations representing two very différent habitats : the Crochatiere and the Magot for brooks and the Pi...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
1996-07-01
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| Series: | Knowledge and Management of Aquatic Ecosystems |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1051/kmae:1996014 |
| _version_ | 1818361665219985408 |
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| author | GRANDJEAN F. SOUTY-GROSSET C. |
| author_facet | GRANDJEAN F. SOUTY-GROSSET C. |
| author_sort | GRANDJEAN F. |
| collection | DOAJ |
| description | Mitochondrial DNA (mtDNA) variation in the white-clawed crayfish, Austropotamobius pallipes pallipes, was examined by restriction endonuclease analysis of samples obtained from three geographical locations representing two very différent habitats : the Crochatiere and the Magot for brooks and the Pinail for ponds. This study used a new type of molecular
marker in crayfish. The method of mtDNA extraction was not based on the clear lysate
method or on ultracentrifugation and used no end-labelling détection. It is discussed according
to the literature about marine crustaceans. MtDNA was digested with 6 endonucleases.
The molécule size of the mtDNA of the white-clawed crayfish was approximately 17750 ± 580
base pairs. Two restriction enzymes produced polymorphic digestion patterns, defining
a total of three haplotypes (1-3). There was a shared haplotype 2 between individuals
among the two brooks. The haplotype 3 was only found in individuals obtained from ponds of Pinail. |
| first_indexed | 2024-12-13T21:20:18Z |
| format | Article |
| id | doaj.art-c93bc975ec0d45dd995682d0af1bd9c6 |
| institution | Directory Open Access Journal |
| issn | 1961-9502 |
| language | English |
| last_indexed | 2024-12-13T21:20:18Z |
| publishDate | 1996-07-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | Knowledge and Management of Aquatic Ecosystems |
| spelling | doaj.art-c93bc975ec0d45dd995682d0af1bd9c62022-12-21T23:31:08ZengEDP SciencesKnowledge and Management of Aquatic Ecosystems1961-95021996-07-01034317518210.1051/kmae:1996014kmae199634302Isolation and characterization of mitochondrial DNA from the endangered white-clawed crayfish Austropotamobius pallipes pallipes, Lereboullet, 1858GRANDJEAN F.SOUTY-GROSSET C.Mitochondrial DNA (mtDNA) variation in the white-clawed crayfish, Austropotamobius pallipes pallipes, was examined by restriction endonuclease analysis of samples obtained from three geographical locations representing two very différent habitats : the Crochatiere and the Magot for brooks and the Pinail for ponds. This study used a new type of molecular marker in crayfish. The method of mtDNA extraction was not based on the clear lysate method or on ultracentrifugation and used no end-labelling détection. It is discussed according to the literature about marine crustaceans. MtDNA was digested with 6 endonucleases. The molécule size of the mtDNA of the white-clawed crayfish was approximately 17750 ± 580 base pairs. Two restriction enzymes produced polymorphic digestion patterns, defining a total of three haplotypes (1-3). There was a shared haplotype 2 between individuals among the two brooks. The haplotype 3 was only found in individuals obtained from ponds of Pinail.http://dx.doi.org/10.1051/kmae:1996014austropotamobius paliipesmtdnagenetic variationrflp |
| spellingShingle | GRANDJEAN F. SOUTY-GROSSET C. Isolation and characterization of mitochondrial DNA from the endangered white-clawed crayfish Austropotamobius pallipes pallipes, Lereboullet, 1858 Knowledge and Management of Aquatic Ecosystems austropotamobius paliipes mtdna genetic variation rflp |
| title | Isolation and characterization of mitochondrial DNA from the endangered white-clawed crayfish Austropotamobius pallipes pallipes, Lereboullet, 1858 |
| title_full | Isolation and characterization of mitochondrial DNA from the endangered white-clawed crayfish Austropotamobius pallipes pallipes, Lereboullet, 1858 |
| title_fullStr | Isolation and characterization of mitochondrial DNA from the endangered white-clawed crayfish Austropotamobius pallipes pallipes, Lereboullet, 1858 |
| title_full_unstemmed | Isolation and characterization of mitochondrial DNA from the endangered white-clawed crayfish Austropotamobius pallipes pallipes, Lereboullet, 1858 |
| title_short | Isolation and characterization of mitochondrial DNA from the endangered white-clawed crayfish Austropotamobius pallipes pallipes, Lereboullet, 1858 |
| title_sort | isolation and characterization of mitochondrial dna from the endangered white clawed crayfish austropotamobius pallipes pallipes lereboullet 1858 |
| topic | austropotamobius paliipes mtdna genetic variation rflp |
| url | http://dx.doi.org/10.1051/kmae:1996014 |
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