Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq
As an important factor affecting the edible quality of peanut kernels, sucrose content is a complex quantitative trait regulated by multiple factors. In this study, an F2 segregating population and a recombinant inbred line (RIL) population, derived from a cross between the high sucrose content vari...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-01-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.1089389/full |
| _version_ | 1797962298716848128 |
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| author | Junjia Guo Feiyan Qi Li Qin Maoning Zhang Ziqi Sun Hongyan Li Mengjie Cui Mengyuan Zhang Chenyu Li Xiaona Li Qi Zhao Dandan Luo Mengdi Tian Hua Liu Jing Xu Lijuan Miao Bingyan Huang Wenzhao Dong Suoyi Han Xinyou Zhang |
| author_facet | Junjia Guo Feiyan Qi Li Qin Maoning Zhang Ziqi Sun Hongyan Li Mengjie Cui Mengyuan Zhang Chenyu Li Xiaona Li Qi Zhao Dandan Luo Mengdi Tian Hua Liu Jing Xu Lijuan Miao Bingyan Huang Wenzhao Dong Suoyi Han Xinyou Zhang |
| author_sort | Junjia Guo |
| collection | DOAJ |
| description | As an important factor affecting the edible quality of peanut kernels, sucrose content is a complex quantitative trait regulated by multiple factors. In this study, an F2 segregating population and a recombinant inbred line (RIL) population, derived from a cross between the high sucrose content variety Jihuatian 1 and the low sucrose content line PI478819, were used as materials to map a quantitative trait locus (QTL) associated with sucrose content in peanut kernels. Four QTLs were initially located on chromosomes A03 and A06 based on BSA-seq technology, and multiple kompetitive allele-specific PCR markers were developed based on single-nucleotide polymorphisms (SNPs) in the intervals. The markers were genotyped in the RIL population and finely mapped to a stable QTL, qSUCA06, located on chromosome A06 within a 0.29-Mb physical genomic interval (112367085–112662675 bp), which accounted for 31.95%–41.05% of the phenotypic variance explained. SNP and insertion/deletion annotations were performed on genes in the candidate interval, and having screened out those genes with mutations in exons, candidate genes were verified by qRT-PCR. The results revealed that Arahy.Y2LWD9 may be the main gene regulating sucrose content. The QTL identified in this study will not only contribute to marker-assisted breeding for improvement of peanut sucrose content but also paves the way for identifying gene function. |
| first_indexed | 2024-04-11T01:11:12Z |
| format | Article |
| id | doaj.art-c93d8658ed684b1fbf4f7dd9c6c4bd44 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T01:11:12Z |
| publishDate | 2023-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-c93d8658ed684b1fbf4f7dd9c6c4bd442023-01-04T05:54:01ZengFrontiers Media S.A.Frontiers in Genetics1664-80212023-01-011310.3389/fgene.2022.10893891089389Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seqJunjia GuoFeiyan QiLi QinMaoning ZhangZiqi SunHongyan LiMengjie CuiMengyuan ZhangChenyu LiXiaona LiQi ZhaoDandan LuoMengdi TianHua LiuJing XuLijuan MiaoBingyan HuangWenzhao DongSuoyi HanXinyou ZhangAs an important factor affecting the edible quality of peanut kernels, sucrose content is a complex quantitative trait regulated by multiple factors. In this study, an F2 segregating population and a recombinant inbred line (RIL) population, derived from a cross between the high sucrose content variety Jihuatian 1 and the low sucrose content line PI478819, were used as materials to map a quantitative trait locus (QTL) associated with sucrose content in peanut kernels. Four QTLs were initially located on chromosomes A03 and A06 based on BSA-seq technology, and multiple kompetitive allele-specific PCR markers were developed based on single-nucleotide polymorphisms (SNPs) in the intervals. The markers were genotyped in the RIL population and finely mapped to a stable QTL, qSUCA06, located on chromosome A06 within a 0.29-Mb physical genomic interval (112367085–112662675 bp), which accounted for 31.95%–41.05% of the phenotypic variance explained. SNP and insertion/deletion annotations were performed on genes in the candidate interval, and having screened out those genes with mutations in exons, candidate genes were verified by qRT-PCR. The results revealed that Arahy.Y2LWD9 may be the main gene regulating sucrose content. The QTL identified in this study will not only contribute to marker-assisted breeding for improvement of peanut sucrose content but also paves the way for identifying gene function.https://www.frontiersin.org/articles/10.3389/fgene.2022.1089389/fullpeanutsucrose contentBSA-seqQTLKASP |
| spellingShingle | Junjia Guo Feiyan Qi Li Qin Maoning Zhang Ziqi Sun Hongyan Li Mengjie Cui Mengyuan Zhang Chenyu Li Xiaona Li Qi Zhao Dandan Luo Mengdi Tian Hua Liu Jing Xu Lijuan Miao Bingyan Huang Wenzhao Dong Suoyi Han Xinyou Zhang Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq Frontiers in Genetics peanut sucrose content BSA-seq QTL KASP |
| title | Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq |
| title_full | Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq |
| title_fullStr | Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq |
| title_full_unstemmed | Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq |
| title_short | Mapping of a QTL associated with sucrose content in peanut kernels using BSA-seq |
| title_sort | mapping of a qtl associated with sucrose content in peanut kernels using bsa seq |
| topic | peanut sucrose content BSA-seq QTL KASP |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.1089389/full |
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