Abscisic acid-polyacrylamide (ABA-PAM) treatment enhances forage grass growth and soil microbial diversity under drought stress
Drought restricts the growth of alpine grassland vegetation. This study aimed to explore a new technical system to improve the drought resistance of forage grass. Qinghai cold-land Poa pratensis seedlings were used in the drought stress experiment. A combination of abscisic acid (ABA) and polyacryla...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.973665/full |
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| author | Xue Tang Xueting Fei Xueting Fei Yining Sun Huanhuan Shao Jinyu Zhu Xinyi He Xiaoyan Wang Bin Yong Xiang Tao |
| author_facet | Xue Tang Xueting Fei Xueting Fei Yining Sun Huanhuan Shao Jinyu Zhu Xinyi He Xiaoyan Wang Bin Yong Xiang Tao |
| author_sort | Xue Tang |
| collection | DOAJ |
| description | Drought restricts the growth of alpine grassland vegetation. This study aimed to explore a new technical system to improve the drought resistance of forage grass. Qinghai cold-land Poa pratensis seedlings were used in the drought stress experiment. A combination of abscisic acid (ABA) and polyacrylamide (PAM) were used to affect the growth, leaf physiology, soil enzyme activity, and rhizosphere microbial diversity of P. pratensis. The fresh leaf weight and root surface area were significantly increased after ABA-PAM combined treatment, while root length was significantly reduced. Besides, the leaf catalase (CAT) and superoxide dismutase (SOD) enzyme activity, proline and chlorophyll content, increased after the treatment, while malondialdehyde (MDA) content decreased. The treatment also increased sucrase, urease, and alkaline protease activities in rhizosphere soil, while decreasing acid phosphatase and neutral phosphatase enzyme activities. ABA-PAM combined treatment enhanced the rhizosphere microbial community and forage drought resistance by altering the abundance of various dominant microorganisms in the rhizosphere soil. The relative abundances of Actinobacteria, Chloroflexi, and Acidobacteria decreased, while Proteobacteria, Firmicutes, and Ascomycota increased. Unlike the relative abundance of Gibberella that decreased significantly, Komagataeibacter, Lactobacillus, Pichia, and Dekkera were significantly increased. Single-factor collinearity network analysis revealed a close relationship between the different rhizosphere microbial communities of forage grass, after ABA-PAM treatment. This study implies that ABA-PAM combined treatment can improve the drought resistance of forages. Therefore, it provides a theoretical and practical basis for restoring drought-induced grassland degradation. |
| first_indexed | 2024-04-13T01:15:56Z |
| format | Article |
| id | doaj.art-c93db125ba6546aabd809935aabfbc64 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T01:15:56Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-c93db125ba6546aabd809935aabfbc642022-12-22T03:08:55ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-09-011310.3389/fpls.2022.973665973665Abscisic acid-polyacrylamide (ABA-PAM) treatment enhances forage grass growth and soil microbial diversity under drought stressXue Tang0Xueting Fei1Xueting Fei2Yining Sun3Huanhuan Shao4Jinyu Zhu5Xinyi He6Xiaoyan Wang7Bin Yong8Xiang Tao9College of Life Sciences, Sichuan Normal University, Chengdu, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu, ChinaLeshan Haitang Experimental Middle School, Leshan, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu, ChinaCollege of Life Sciences, Sichuan Normal University, Chengdu, ChinaDrought restricts the growth of alpine grassland vegetation. This study aimed to explore a new technical system to improve the drought resistance of forage grass. Qinghai cold-land Poa pratensis seedlings were used in the drought stress experiment. A combination of abscisic acid (ABA) and polyacrylamide (PAM) were used to affect the growth, leaf physiology, soil enzyme activity, and rhizosphere microbial diversity of P. pratensis. The fresh leaf weight and root surface area were significantly increased after ABA-PAM combined treatment, while root length was significantly reduced. Besides, the leaf catalase (CAT) and superoxide dismutase (SOD) enzyme activity, proline and chlorophyll content, increased after the treatment, while malondialdehyde (MDA) content decreased. The treatment also increased sucrase, urease, and alkaline protease activities in rhizosphere soil, while decreasing acid phosphatase and neutral phosphatase enzyme activities. ABA-PAM combined treatment enhanced the rhizosphere microbial community and forage drought resistance by altering the abundance of various dominant microorganisms in the rhizosphere soil. The relative abundances of Actinobacteria, Chloroflexi, and Acidobacteria decreased, while Proteobacteria, Firmicutes, and Ascomycota increased. Unlike the relative abundance of Gibberella that decreased significantly, Komagataeibacter, Lactobacillus, Pichia, and Dekkera were significantly increased. Single-factor collinearity network analysis revealed a close relationship between the different rhizosphere microbial communities of forage grass, after ABA-PAM treatment. This study implies that ABA-PAM combined treatment can improve the drought resistance of forages. Therefore, it provides a theoretical and practical basis for restoring drought-induced grassland degradation.https://www.frontiersin.org/articles/10.3389/fpls.2022.973665/fullsoil bacterial communityabscisic acidpolyacrylamidedrought stressforage grass |
| spellingShingle | Xue Tang Xueting Fei Xueting Fei Yining Sun Huanhuan Shao Jinyu Zhu Xinyi He Xiaoyan Wang Bin Yong Xiang Tao Abscisic acid-polyacrylamide (ABA-PAM) treatment enhances forage grass growth and soil microbial diversity under drought stress Frontiers in Plant Science soil bacterial community abscisic acid polyacrylamide drought stress forage grass |
| title | Abscisic acid-polyacrylamide (ABA-PAM) treatment enhances forage grass growth and soil microbial diversity under drought stress |
| title_full | Abscisic acid-polyacrylamide (ABA-PAM) treatment enhances forage grass growth and soil microbial diversity under drought stress |
| title_fullStr | Abscisic acid-polyacrylamide (ABA-PAM) treatment enhances forage grass growth and soil microbial diversity under drought stress |
| title_full_unstemmed | Abscisic acid-polyacrylamide (ABA-PAM) treatment enhances forage grass growth and soil microbial diversity under drought stress |
| title_short | Abscisic acid-polyacrylamide (ABA-PAM) treatment enhances forage grass growth and soil microbial diversity under drought stress |
| title_sort | abscisic acid polyacrylamide aba pam treatment enhances forage grass growth and soil microbial diversity under drought stress |
| topic | soil bacterial community abscisic acid polyacrylamide drought stress forage grass |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.973665/full |
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