Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactum
Abstract Trichoderma brevicompactum and T. arundinaceum both can synthesize trichodermin with strong antifungal activity and high biotechnological value. The two Trichoderma species have a tri cluster, which includes seven genes (tri14, tri12, tri11, tri10, tri3, tri4, and tri6) that encode transpor...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-04-01
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| Series: | AMB Express |
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| Online Access: | http://link.springer.com/article/10.1186/s13568-018-0585-4 |
| _version_ | 1818992646094323712 |
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| author | Xuping Shentu Jiayi Yao Xiaofeng Yuan Linmao He Fan Sun Kozo Ochi Xiaoping Yu |
| author_facet | Xuping Shentu Jiayi Yao Xiaofeng Yuan Linmao He Fan Sun Kozo Ochi Xiaoping Yu |
| author_sort | Xuping Shentu |
| collection | DOAJ |
| description | Abstract Trichoderma brevicompactum and T. arundinaceum both can synthesize trichodermin with strong antifungal activity and high biotechnological value. The two Trichoderma species have a tri cluster, which includes seven genes (tri14, tri12, tri11, tri10, tri3, tri4, and tri6) that encode transport and regulatory enzymes required for the biosynthesis of trichodermin. Here, we isolated T. brevicompactum 0248 transformants with disrupted tri11, tri4, or tri3 gene. We also described the effect of tri11, tri3, or tri4 deletion on the expression of other genes in the tri cluster. Targeted Δtri3 knockout mutant exhibited a sharp decline in the production of trichodermin, and trichodermol, which is a substrate for trichodermin production, accumulated. Thus, the results demonstrated that tri3 was responsible for the biosynthesis of trichodermin, and the tri3 gene-encoded enzyme catalyzed the acetylation reaction of the hydroxy group at C-4 of the trichodermin skeleton. In addition, tri4 and tri11 deletion mutants were generated to evaluate the roles of tri4 and tri11 in trichodermin biosynthesis, respectively. Deletion mutant strain Δtri4 or Δtri11 did not produce trichodermin in T. brevicompactum, indicating that tri4 and tri11 are essential for trichodermin biosynthesis. This is the first to report the function of tri3, tri4 and tri11 in T. brevicompactum, although the role of tri4 and tri11 has already been described for T. arundinaceum by Cardoza et al. (Appl Environ Microbiol 77:4867–4877, 2011). |
| first_indexed | 2024-12-20T20:29:28Z |
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| id | doaj.art-c93de255f6ed4abd97eb7271686dfd9f |
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| issn | 2191-0855 |
| language | English |
| last_indexed | 2024-12-20T20:29:28Z |
| publishDate | 2018-04-01 |
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| series | AMB Express |
| spelling | doaj.art-c93de255f6ed4abd97eb7271686dfd9f2022-12-21T19:27:23ZengSpringerOpenAMB Express2191-08552018-04-018111110.1186/s13568-018-0585-4Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactumXuping Shentu0Jiayi Yao1Xiaofeng Yuan2Linmao He3Fan Sun4Kozo Ochi5Xiaoping Yu6Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang UniversityZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang UniversityZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang UniversityZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang UniversityZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang UniversityDepartment of Life Science, Hiroshima Institute of TechnologyZhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang UniversityAbstract Trichoderma brevicompactum and T. arundinaceum both can synthesize trichodermin with strong antifungal activity and high biotechnological value. The two Trichoderma species have a tri cluster, which includes seven genes (tri14, tri12, tri11, tri10, tri3, tri4, and tri6) that encode transport and regulatory enzymes required for the biosynthesis of trichodermin. Here, we isolated T. brevicompactum 0248 transformants with disrupted tri11, tri4, or tri3 gene. We also described the effect of tri11, tri3, or tri4 deletion on the expression of other genes in the tri cluster. Targeted Δtri3 knockout mutant exhibited a sharp decline in the production of trichodermin, and trichodermol, which is a substrate for trichodermin production, accumulated. Thus, the results demonstrated that tri3 was responsible for the biosynthesis of trichodermin, and the tri3 gene-encoded enzyme catalyzed the acetylation reaction of the hydroxy group at C-4 of the trichodermin skeleton. In addition, tri4 and tri11 deletion mutants were generated to evaluate the roles of tri4 and tri11 in trichodermin biosynthesis, respectively. Deletion mutant strain Δtri4 or Δtri11 did not produce trichodermin in T. brevicompactum, indicating that tri4 and tri11 are essential for trichodermin biosynthesis. This is the first to report the function of tri3, tri4 and tri11 in T. brevicompactum, although the role of tri4 and tri11 has already been described for T. arundinaceum by Cardoza et al. (Appl Environ Microbiol 77:4867–4877, 2011).http://link.springer.com/article/10.1186/s13568-018-0585-4Trichoderma brevicompactumtri3tri4tri11Gene deletionTrichodermin |
| spellingShingle | Xuping Shentu Jiayi Yao Xiaofeng Yuan Linmao He Fan Sun Kozo Ochi Xiaoping Yu Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactum AMB Express Trichoderma brevicompactum tri3 tri4 tri11 Gene deletion Trichodermin |
| title | Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactum |
| title_full | Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactum |
| title_fullStr | Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactum |
| title_full_unstemmed | Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactum |
| title_short | Tri11, tri3, and tri4 genes are required for trichodermin biosynthesis of Trichoderma brevicompactum |
| title_sort | tri11 tri3 and tri4 genes are required for trichodermin biosynthesis of trichoderma brevicompactum |
| topic | Trichoderma brevicompactum tri3 tri4 tri11 Gene deletion Trichodermin |
| url | http://link.springer.com/article/10.1186/s13568-018-0585-4 |
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