Pooled-Peptide Epitope Mapping Strategies Are Efficient and Highly Sensitive: An Evaluation of Methods for Identifying Human T Cell Epitope Specificities in Large-Scale HIV Vaccine Efficacy Trials.
The interferon gamma, enzyme-linked immunospot (IFN-γ ELISpot) assay is widely used to identify viral antigen-specific T cells is frequently employed to quantify T cell responses in HIV vaccine studies. It can be used to define T cell epitope specificities using panels of peptide antigens, but with...
Main Authors: | Andrew Fiore-Gartland, Bryce A Manso, David P Friedrich, Erin E Gabriel, Greg Finak, Zoe Moodie, Tomer Hertz, Stephen C De Rosa, Nicole Frahm, Peter B Gilbert, M Juliana McElrath |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2016-01-01
|
Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC4749288?pdf=render |
Similar Items
-
Effective Antiviral CD8+T Cells Responses Are Rare in HIVPositive Step and Phambili Study Participants but Are Targeted to Low Entropy Viral Epitopes
by: Hancock, G, et al.
Published: (2013) -
Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes.
by: Beattie, T, et al.
Published: (2004) -
Cohort-Specific Peptide Reagents Broaden Depth and Breadth Estimates of the CD8 T Cell Response to HIV-1 Gag Potential T Cell Epitopes
by: Clive M. Michelo, et al.
Published: (2023-02-01) -
Polytopic fractional delivery of an HIV vaccine alters cellular responses and results in increased epitope breadth in a phase 1 randomized trialResearch in context
by: Maurine D. Miner, et al.
Published: (2024-02-01) -
ASFV epitope mapping by high density peptides microarrays
by: Cloé Desmet, et al.
Published: (2024-01-01)