Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.)
Mulberry (Morus alba L.) represents one of the most commonly utilized plants in traditional medicine and as a nutritional plant used worldwide. The polyhydroxylated alkaloid 1-deoxynojirimycin (DNJ) is the major bioactive compounds of mulberry in treating diabetes. However, the DNJ content in mulber...
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PeerJ Inc.
2018-08-01
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| Online Access: | https://peerj.com/articles/5443.pdf |
| _version_ | 1797418277656330240 |
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| author | Dujun Wang Li Zhao Dan Wang Jia Liu Xiaofeng Yu Yuan Wei Zhen Ouyang |
| author_facet | Dujun Wang Li Zhao Dan Wang Jia Liu Xiaofeng Yu Yuan Wei Zhen Ouyang |
| author_sort | Dujun Wang |
| collection | DOAJ |
| description | Mulberry (Morus alba L.) represents one of the most commonly utilized plants in traditional medicine and as a nutritional plant used worldwide. The polyhydroxylated alkaloid 1-deoxynojirimycin (DNJ) is the major bioactive compounds of mulberry in treating diabetes. However, the DNJ content in mulberry is very low. Therefore, identification of key genes involved in DNJ alkaloid biosynthesis will provide a basis for the further analysis of its biosynthetic pathway and ultimately for the realization of synthetic biological production. Here, two cDNA libraries of mulberry leaf samples with different DNJ contents were constructed. Approximately 16 Gb raw RNA-Seq data was generated and de novo assembled into 112,481 transcripts, with an average length of 766 bp and an N50 value of 1,392. Subsequently, all unigenes were annotated based on nine public databases; 11,318 transcripts were found to be significantly differentially regulated. A total of 38 unique candidate genes were identified as being involved in DNJ alkaloid biosynthesis in mulberry, and nine unique genes had significantly different expression. Three key transcripts of DNJ biosynthesis were identified and further characterized using RT-PCR; they were assigned to lysine decarboxylase and primary-amine oxidase genes. Five CYP450 transcripts and two methyltransferase transcripts were significantly associated with DNJ content. Overall, the biosynthetic pathway of DNJ alkaloid was preliminarily speculated. |
| first_indexed | 2024-03-09T06:31:27Z |
| format | Article |
| id | doaj.art-c93fe8fd892245aba7367ccf7d3fd218 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:31:27Z |
| publishDate | 2018-08-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-c93fe8fd892245aba7367ccf7d3fd2182023-12-03T11:06:01ZengPeerJ Inc.PeerJ2167-83592018-08-016e544310.7717/peerj.5443Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.)Dujun Wang0Li Zhao1Dan Wang2Jia Liu3Xiaofeng Yu4Yuan Wei5Zhen Ouyang6School of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaSchool of Pharmacy, Jiangsu University, Zhenjiang, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaSchool of Pharmacy, Jiangsu University, Zhenjiang, ChinaSchool of Pharmacy, Jiangsu University, Zhenjiang, ChinaSchool of Food and Biological Engineering, Jiangsu University, Zhenjiang, ChinaMulberry (Morus alba L.) represents one of the most commonly utilized plants in traditional medicine and as a nutritional plant used worldwide. The polyhydroxylated alkaloid 1-deoxynojirimycin (DNJ) is the major bioactive compounds of mulberry in treating diabetes. However, the DNJ content in mulberry is very low. Therefore, identification of key genes involved in DNJ alkaloid biosynthesis will provide a basis for the further analysis of its biosynthetic pathway and ultimately for the realization of synthetic biological production. Here, two cDNA libraries of mulberry leaf samples with different DNJ contents were constructed. Approximately 16 Gb raw RNA-Seq data was generated and de novo assembled into 112,481 transcripts, with an average length of 766 bp and an N50 value of 1,392. Subsequently, all unigenes were annotated based on nine public databases; 11,318 transcripts were found to be significantly differentially regulated. A total of 38 unique candidate genes were identified as being involved in DNJ alkaloid biosynthesis in mulberry, and nine unique genes had significantly different expression. Three key transcripts of DNJ biosynthesis were identified and further characterized using RT-PCR; they were assigned to lysine decarboxylase and primary-amine oxidase genes. Five CYP450 transcripts and two methyltransferase transcripts were significantly associated with DNJ content. Overall, the biosynthetic pathway of DNJ alkaloid was preliminarily speculated.https://peerj.com/articles/5443.pdfMulberryTranscriptomic1-deoxynojirimycinBiosynthesisKey enzyme gene analysis |
| spellingShingle | Dujun Wang Li Zhao Dan Wang Jia Liu Xiaofeng Yu Yuan Wei Zhen Ouyang Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.) PeerJ Mulberry Transcriptomic 1-deoxynojirimycin Biosynthesis Key enzyme gene analysis |
| title | Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.) |
| title_full | Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.) |
| title_fullStr | Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.) |
| title_full_unstemmed | Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.) |
| title_short | Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry (Morus alba L.) |
| title_sort | transcriptome analysis and identification of key genes involved in 1 deoxynojirimycin biosynthesis of mulberry morus alba l |
| topic | Mulberry Transcriptomic 1-deoxynojirimycin Biosynthesis Key enzyme gene analysis |
| url | https://peerj.com/articles/5443.pdf |
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