Optically transparent vertical silicon nanowire arrays for live-cell imaging
Abstract Programmable nano-bio interfaces driven by tuneable vertically configured nanostructures have recently emerged as a powerful tool for cellular manipulations and interrogations. Such interfaces have strong potential for ground-breaking advances, particularly in cellular nanobiotechnology and...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-02-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-021-00795-7 |
| _version_ | 1811186575665528832 |
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| author | Roey Elnathan Andrew W. Holle Jennifer Young Marina A. George Omri Heifler Andriy Goychuk Erwin Frey Ralf Kemkemer Joachim P. Spatz Alon Kosloff Fernando Patolsky Nicolas H. Voelcker |
| author_facet | Roey Elnathan Andrew W. Holle Jennifer Young Marina A. George Omri Heifler Andriy Goychuk Erwin Frey Ralf Kemkemer Joachim P. Spatz Alon Kosloff Fernando Patolsky Nicolas H. Voelcker |
| author_sort | Roey Elnathan |
| collection | DOAJ |
| description | Abstract Programmable nano-bio interfaces driven by tuneable vertically configured nanostructures have recently emerged as a powerful tool for cellular manipulations and interrogations. Such interfaces have strong potential for ground-breaking advances, particularly in cellular nanobiotechnology and mechanobiology. However, the opaque nature of many nanostructured surfaces makes non-destructive, live-cell characterization of cellular behavior on vertically aligned nanostructures challenging to observe. Here, a new nanofabrication route is proposed that enables harvesting of vertically aligned silicon (Si) nanowires and their subsequent transfer onto an optically transparent substrate, with high efficiency and without artefacts. We demonstrate the potential of this route for efficient live-cell phase contrast imaging and subsequent characterization of cells growing on vertically aligned Si nanowires. This approach provides the first opportunity to understand dynamic cellular responses to a cell-nanowire interface, and thus has the potential to inform the design of future nanoscale cellular manipulation technologies. |
| first_indexed | 2024-04-11T13:47:44Z |
| format | Article |
| id | doaj.art-c9401eb7c7484b61be5d1d691f4e1ddf |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T13:47:44Z |
| publishDate | 2021-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-c9401eb7c7484b61be5d1d691f4e1ddf2022-12-22T04:20:56ZengBMCJournal of Nanobiotechnology1477-31552021-02-011911610.1186/s12951-021-00795-7Optically transparent vertical silicon nanowire arrays for live-cell imagingRoey Elnathan0Andrew W. Holle1Jennifer Young2Marina A. George3Omri Heifler4Andriy Goychuk5Erwin Frey6Ralf Kemkemer7Joachim P. Spatz8Alon Kosloff9Fernando Patolsky10Nicolas H. Voelcker11Faculty of Pharmacy and Pharmaceutical Sciences, Monash UniversityMechanobiology Institute, National University of SingaporeMechanobiology Institute, National University of SingaporeDepartment of Materials Science and Engineering, Monash UniversitySchool of Chemistry, The Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv UniversityArnold Sommerfeld Center for Theoretical Physics and Center for NanoScience, Department of Physics, Ludwig-Maximilians-Universität MünchenArnold Sommerfeld Center for Theoretical Physics and Center for NanoScience, Department of Physics, Ludwig-Maximilians-Universität MünchenDepartment of Cellular Biophysics, Max Planck Institute for Medical ResearchDepartment of Cellular Biophysics, Max Planck Institute for Medical ResearchSchool of Chemistry, The Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv UniversitySchool of Chemistry, The Raymond and Beverly Sackler Faculty of Exact Sciences, Tel-Aviv UniversityFaculty of Pharmacy and Pharmaceutical Sciences, Monash UniversityAbstract Programmable nano-bio interfaces driven by tuneable vertically configured nanostructures have recently emerged as a powerful tool for cellular manipulations and interrogations. Such interfaces have strong potential for ground-breaking advances, particularly in cellular nanobiotechnology and mechanobiology. However, the opaque nature of many nanostructured surfaces makes non-destructive, live-cell characterization of cellular behavior on vertically aligned nanostructures challenging to observe. Here, a new nanofabrication route is proposed that enables harvesting of vertically aligned silicon (Si) nanowires and their subsequent transfer onto an optically transparent substrate, with high efficiency and without artefacts. We demonstrate the potential of this route for efficient live-cell phase contrast imaging and subsequent characterization of cells growing on vertically aligned Si nanowires. This approach provides the first opportunity to understand dynamic cellular responses to a cell-nanowire interface, and thus has the potential to inform the design of future nanoscale cellular manipulation technologies.https://doi.org/10.1186/s12951-021-00795-7NanowiresCell–material interfaceLive-cell phase-contrast imagingSiliconGlass substrate |
| spellingShingle | Roey Elnathan Andrew W. Holle Jennifer Young Marina A. George Omri Heifler Andriy Goychuk Erwin Frey Ralf Kemkemer Joachim P. Spatz Alon Kosloff Fernando Patolsky Nicolas H. Voelcker Optically transparent vertical silicon nanowire arrays for live-cell imaging Journal of Nanobiotechnology Nanowires Cell–material interface Live-cell phase-contrast imaging Silicon Glass substrate |
| title | Optically transparent vertical silicon nanowire arrays for live-cell imaging |
| title_full | Optically transparent vertical silicon nanowire arrays for live-cell imaging |
| title_fullStr | Optically transparent vertical silicon nanowire arrays for live-cell imaging |
| title_full_unstemmed | Optically transparent vertical silicon nanowire arrays for live-cell imaging |
| title_short | Optically transparent vertical silicon nanowire arrays for live-cell imaging |
| title_sort | optically transparent vertical silicon nanowire arrays for live cell imaging |
| topic | Nanowires Cell–material interface Live-cell phase-contrast imaging Silicon Glass substrate |
| url | https://doi.org/10.1186/s12951-021-00795-7 |
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