Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae)
Analysis of pooled genomic short read sequence data revealed the presence of nudivirus-derived sequences from U.S. populations of both southern corn rootworm (SCR, <i>Diabrotica undecimpunctata howardi</i> Barber) and western corn rootworm (WCR, <i>Diabrotica virgifera virgifera<...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/13/2/269 |
| _version_ | 1827601442675884032 |
|---|---|
| author | Sijun Liu Thomas W. Sappington Brad S. Coates Bryony C. Bonning |
| author_facet | Sijun Liu Thomas W. Sappington Brad S. Coates Bryony C. Bonning |
| author_sort | Sijun Liu |
| collection | DOAJ |
| description | Analysis of pooled genomic short read sequence data revealed the presence of nudivirus-derived sequences from U.S. populations of both southern corn rootworm (SCR, <i>Diabrotica undecimpunctata howardi</i> Barber) and western corn rootworm (WCR, <i>Diabrotica virgifera virgifera</i> LeConte). A near complete nudivirus genome sequence was assembled from sequence data for an SCR population with relatively high viral titers. A total of 147,179 bp was assembled from five contigs that collectively encode 109 putative open reading frames (ORFs) including 20 nudivirus core genes. In contrast, genome sequence recovery was incomplete for a second nudivirus from WCR, although sequences derived from this virus were present in three geographically dispersed populations. Only 48,989 bp were assembled with 48 putative ORFs including 13 core genes, representing about 20% of a typical nudivirus genome. Phylogenetic analysis indicated that both corn rootworm nudiviruses grouped with the third known nudivirus of beetles, <i>Oryctes rhinoceros nudivirus</i> in the genus <i>Alphanudivirus</i>. On the basis of phylogenetic and additional analyses, we propose further taxonomic separation of nudiviruses within <i>Alphanudivirus</i> and <i>Betanudivirus</i> into two subfamilies and five genera. Identification of nudivirus-derived sequences from two species of corn rootworm highlights the diversity of viruses associated with these agricultural insect pests. |
| first_indexed | 2024-03-09T04:55:27Z |
| format | Article |
| id | doaj.art-c9403c0cc546431f823509e180f180d0 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T04:55:27Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-c9403c0cc546431f823509e180f180d02023-12-03T13:06:39ZengMDPI AGViruses1999-49152021-02-0113226910.3390/v13020269Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae)Sijun Liu0Thomas W. Sappington1Brad S. Coates2Bryony C. Bonning3Department of Entomology, Iowa State University, Ames, IA 50011, USACorn Insects and Crop Genetics Research Unit, USDA-ARS, Ames, IA 50011, USACorn Insects and Crop Genetics Research Unit, USDA-ARS, Ames, IA 50011, USADepartment of Entomology and Nematology, University of Florida, Gainesville, FL 32611, USAAnalysis of pooled genomic short read sequence data revealed the presence of nudivirus-derived sequences from U.S. populations of both southern corn rootworm (SCR, <i>Diabrotica undecimpunctata howardi</i> Barber) and western corn rootworm (WCR, <i>Diabrotica virgifera virgifera</i> LeConte). A near complete nudivirus genome sequence was assembled from sequence data for an SCR population with relatively high viral titers. A total of 147,179 bp was assembled from five contigs that collectively encode 109 putative open reading frames (ORFs) including 20 nudivirus core genes. In contrast, genome sequence recovery was incomplete for a second nudivirus from WCR, although sequences derived from this virus were present in three geographically dispersed populations. Only 48,989 bp were assembled with 48 putative ORFs including 13 core genes, representing about 20% of a typical nudivirus genome. Phylogenetic analysis indicated that both corn rootworm nudiviruses grouped with the third known nudivirus of beetles, <i>Oryctes rhinoceros nudivirus</i> in the genus <i>Alphanudivirus</i>. On the basis of phylogenetic and additional analyses, we propose further taxonomic separation of nudiviruses within <i>Alphanudivirus</i> and <i>Betanudivirus</i> into two subfamilies and five genera. Identification of nudivirus-derived sequences from two species of corn rootworm highlights the diversity of viruses associated with these agricultural insect pests.https://www.mdpi.com/1999-4915/13/2/269<i>Nudiviridae</i><i>Alphanudivirus</i>beetlecorn rootworm<i>Diabrotica</i> spp. |
| spellingShingle | Sijun Liu Thomas W. Sappington Brad S. Coates Bryony C. Bonning Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae) Viruses <i>Nudiviridae</i> <i>Alphanudivirus</i> beetle corn rootworm <i>Diabrotica</i> spp. |
| title | Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae) |
| title_full | Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae) |
| title_fullStr | Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae) |
| title_full_unstemmed | Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae) |
| title_short | Nudivirus Sequences Identified from the Southern and Western Corn Rootworms (Coleoptera: Chrysomelidae) |
| title_sort | nudivirus sequences identified from the southern and western corn rootworms coleoptera chrysomelidae |
| topic | <i>Nudiviridae</i> <i>Alphanudivirus</i> beetle corn rootworm <i>Diabrotica</i> spp. |
| url | https://www.mdpi.com/1999-4915/13/2/269 |
| work_keys_str_mv | AT sijunliu nudivirussequencesidentifiedfromthesouthernandwesterncornrootwormscoleopterachrysomelidae AT thomaswsappington nudivirussequencesidentifiedfromthesouthernandwesterncornrootwormscoleopterachrysomelidae AT bradscoates nudivirussequencesidentifiedfromthesouthernandwesterncornrootwormscoleopterachrysomelidae AT bryonycbonning nudivirussequencesidentifiedfromthesouthernandwesterncornrootwormscoleopterachrysomelidae |