Identification of Pathogens Causing Alfalfa Fusarium Root Rot in Inner Mongolia, China
Alfalfa Fusarium Root Rot (AFRR) is a serious soil-borne disease with a complex pathogenicity. Diseased samples suspected of AFRR were collected from Hohhot, Ordos, Hulunbeier, Chifeng, and Bayannur in Inner Mongolia, China, leading to 317 isolates. The isolates were identified as <i>Fusarium...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/2/456 |
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| author | Le Wang Na Wang Jialiang Yu Jie Wu Huan Liu Kejian Lin Yuanyuan Zhang |
| author_facet | Le Wang Na Wang Jialiang Yu Jie Wu Huan Liu Kejian Lin Yuanyuan Zhang |
| author_sort | Le Wang |
| collection | DOAJ |
| description | Alfalfa Fusarium Root Rot (AFRR) is a serious soil-borne disease with a complex pathogenicity. Diseased samples suspected of AFRR were collected from Hohhot, Ordos, Hulunbeier, Chifeng, and Bayannur in Inner Mongolia, China, leading to 317 isolates. The isolates were identified as <i>Fusarium acuminatum</i>, <i>F. solani</i>, <i>F. equiseti</i>, <i>F. incarnatum</i>, <i>F. oxysporum</i>, <i>F. avenaceum</i>, <i>F. verticillioides</i>, <i>F. proliferatum</i>, <i>F. falciforme</i>, <i>F. tricinctum</i>, <i>F. virguliforme</i>, and <i>F. redolens</i>, and the results of pathogenicity testing showed that 12 <i>Fusarium</i> species could cause alfalfa root rot. Among these, <i>F. verticillioides</i>, <i>F. falciforme</i>, and <i>F. virguliforme</i> have not previously been reported to cause AFRR in China. Although the population structure of the pathogens differed in different regions, the dominant pathogenic species was <i>F. acuminatum</i>. Fungicide toxicity tests showed that seven fungicides inhibited <i>F. acuminatum</i>, of which fludioxonil, kresoxim-methyl, and triadimefon were found to be strongly toxic towards <i>F. acuminatum</i> with EC<sub>50</sub> values of 0.09, 2.28, and 16.37 μg/mL, respectively, suggesting that these could be used as alternative fungicides for the control of AFRR. The results of this study can provide a theoretical basis for exploring the occurrence and epidemiology of alfalfa root rot and strategies for its control. |
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| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T09:17:20Z |
| publishDate | 2023-02-01 |
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| series | Agronomy |
| spelling | doaj.art-c940730a1188417a8cc66dde0ab9465d2023-11-16T18:34:52ZengMDPI AGAgronomy2073-43952023-02-0113245610.3390/agronomy13020456Identification of Pathogens Causing Alfalfa Fusarium Root Rot in Inner Mongolia, ChinaLe Wang0Na Wang1Jialiang Yu2Jie Wu3Huan Liu4Kejian Lin5Yuanyuan Zhang6Key Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaCollege of Horticulture and Plant Protection, Inner Mongolia Agricultural University, Hohhot 010018, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaKey Laboratory of Biohazard Monitoring, Green Prevention and Control for Artificial Grassland, Ministry of Agriculture and Rural Affairs, Institute of Grassland Research of Chinese Academy of Agricultural Sciences, Hohhot 010010, ChinaAlfalfa Fusarium Root Rot (AFRR) is a serious soil-borne disease with a complex pathogenicity. Diseased samples suspected of AFRR were collected from Hohhot, Ordos, Hulunbeier, Chifeng, and Bayannur in Inner Mongolia, China, leading to 317 isolates. The isolates were identified as <i>Fusarium acuminatum</i>, <i>F. solani</i>, <i>F. equiseti</i>, <i>F. incarnatum</i>, <i>F. oxysporum</i>, <i>F. avenaceum</i>, <i>F. verticillioides</i>, <i>F. proliferatum</i>, <i>F. falciforme</i>, <i>F. tricinctum</i>, <i>F. virguliforme</i>, and <i>F. redolens</i>, and the results of pathogenicity testing showed that 12 <i>Fusarium</i> species could cause alfalfa root rot. Among these, <i>F. verticillioides</i>, <i>F. falciforme</i>, and <i>F. virguliforme</i> have not previously been reported to cause AFRR in China. Although the population structure of the pathogens differed in different regions, the dominant pathogenic species was <i>F. acuminatum</i>. Fungicide toxicity tests showed that seven fungicides inhibited <i>F. acuminatum</i>, of which fludioxonil, kresoxim-methyl, and triadimefon were found to be strongly toxic towards <i>F. acuminatum</i> with EC<sub>50</sub> values of 0.09, 2.28, and 16.37 μg/mL, respectively, suggesting that these could be used as alternative fungicides for the control of AFRR. The results of this study can provide a theoretical basis for exploring the occurrence and epidemiology of alfalfa root rot and strategies for its control.https://www.mdpi.com/2073-4395/13/2/456Alfalfa Fusarium Root Rot<i>Fusarium</i> spp.molecular identificationfungicide |
| spellingShingle | Le Wang Na Wang Jialiang Yu Jie Wu Huan Liu Kejian Lin Yuanyuan Zhang Identification of Pathogens Causing Alfalfa Fusarium Root Rot in Inner Mongolia, China Agronomy Alfalfa Fusarium Root Rot <i>Fusarium</i> spp. molecular identification fungicide |
| title | Identification of Pathogens Causing Alfalfa Fusarium Root Rot in Inner Mongolia, China |
| title_full | Identification of Pathogens Causing Alfalfa Fusarium Root Rot in Inner Mongolia, China |
| title_fullStr | Identification of Pathogens Causing Alfalfa Fusarium Root Rot in Inner Mongolia, China |
| title_full_unstemmed | Identification of Pathogens Causing Alfalfa Fusarium Root Rot in Inner Mongolia, China |
| title_short | Identification of Pathogens Causing Alfalfa Fusarium Root Rot in Inner Mongolia, China |
| title_sort | identification of pathogens causing alfalfa fusarium root rot in inner mongolia china |
| topic | Alfalfa Fusarium Root Rot <i>Fusarium</i> spp. molecular identification fungicide |
| url | https://www.mdpi.com/2073-4395/13/2/456 |
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