Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonnei
Shigella sonnei is the most common Shigella spp. in developed areas and the second most common in undeveloped regions. In this study, a multiple cross displacement amplification (MCDA) assay was used in combination with a lateral flow biosensor (LFB) assay to detect virulent S. sonnei strains contai...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1012105/full |
| _version_ | 1811237710386429952 |
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| author | Yonglu Wang Yonglu Wang Ziqiang He Patigul Ablimit Shunshi Ji Dong Jin |
| author_facet | Yonglu Wang Yonglu Wang Ziqiang He Patigul Ablimit Shunshi Ji Dong Jin |
| author_sort | Yonglu Wang |
| collection | DOAJ |
| description | Shigella sonnei is the most common Shigella spp. in developed areas and the second most common in undeveloped regions. In this study, a multiple cross displacement amplification (MCDA) assay was used in combination with a lateral flow biosensor (LFB) assay to detect virulent S. sonnei strains containing the ipaH and wbgX genes. The multiplex MCDA-LFB assay detected wbgX at ≥1 pg/μL and ipaH at ≥10 fg/μL within 30 min in pure cultures maintained at 63°C. This assay was sensitive for ~37 CFU of virulent S. sonnei and ~3.7 CFU of Shigella spp. and enteroinvasive E. coli in stimulated fecal samples and had 100% specificity among 59 reference strains. The MCDA-LFB assay was also able to differentiate between virulent S. sonnei and other Shigella spp. and enteroinvasive E. coli among 99 clinical isolates. In summary, a multiplex MCDA-LFB assay was developed for rapid, convenient, point-of-care, and accurate identification of virulent S. sonnei within 30 min and at a constant temperature without the need for expensive lab equipment. |
| first_indexed | 2024-04-12T12:28:03Z |
| format | Article |
| id | doaj.art-c941091e9cfd4ad69c4d89c929b0557b |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-12T12:28:03Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-c941091e9cfd4ad69c4d89c929b0557b2022-12-22T03:33:06ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-10-011210.3389/fcimb.2022.10121051012105Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonneiYonglu Wang0Yonglu Wang1Ziqiang He2Patigul Ablimit3Shunshi Ji4Dong Jin5Ma’anshan Center for Disease Control and Prevention, Ma’anshan, ChinaPishan County Center for Disease Control and Prevention, Hotan Prefecture, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaPishan County Center for Disease Control and Prevention, Hotan Prefecture, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaState Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, ChinaShigella sonnei is the most common Shigella spp. in developed areas and the second most common in undeveloped regions. In this study, a multiple cross displacement amplification (MCDA) assay was used in combination with a lateral flow biosensor (LFB) assay to detect virulent S. sonnei strains containing the ipaH and wbgX genes. The multiplex MCDA-LFB assay detected wbgX at ≥1 pg/μL and ipaH at ≥10 fg/μL within 30 min in pure cultures maintained at 63°C. This assay was sensitive for ~37 CFU of virulent S. sonnei and ~3.7 CFU of Shigella spp. and enteroinvasive E. coli in stimulated fecal samples and had 100% specificity among 59 reference strains. The MCDA-LFB assay was also able to differentiate between virulent S. sonnei and other Shigella spp. and enteroinvasive E. coli among 99 clinical isolates. In summary, a multiplex MCDA-LFB assay was developed for rapid, convenient, point-of-care, and accurate identification of virulent S. sonnei within 30 min and at a constant temperature without the need for expensive lab equipment.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1012105/fullvirulent shigella sonneimultiplex cross displacement amplificationlateral flow biosensorsensitivityspecificity |
| spellingShingle | Yonglu Wang Yonglu Wang Ziqiang He Patigul Ablimit Shunshi Ji Dong Jin Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonnei Frontiers in Cellular and Infection Microbiology virulent shigella sonnei multiplex cross displacement amplification lateral flow biosensor sensitivity specificity |
| title | Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonnei |
| title_full | Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonnei |
| title_fullStr | Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonnei |
| title_full_unstemmed | Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonnei |
| title_short | Development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonnei |
| title_sort | development of multiplex cross displacement amplification combined with lateral flow biosensor assay for detection of virulent shigella sonnei |
| topic | virulent shigella sonnei multiplex cross displacement amplification lateral flow biosensor sensitivity specificity |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1012105/full |
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