Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating Peptide
Soft tissue sealing around zirconia (ZrO<sub>2</sub>) abutment is critical for the long-term stability of dental implants. The goal of the study is to develop a strong basal lamina (BL)-mediated epithelial attachment to ZrO<sub>2</sub> via a novel physicochemical immobilizati...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Materials |
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| Online Access: | https://www.mdpi.com/1996-1944/15/13/4597 |
| _version_ | 1797408738834907136 |
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| author | Chia-Yu Chen Wonwoo Jang David M. Kim Masazumi Nagai Shigemi Nagai |
| author_facet | Chia-Yu Chen Wonwoo Jang David M. Kim Masazumi Nagai Shigemi Nagai |
| author_sort | Chia-Yu Chen |
| collection | DOAJ |
| description | Soft tissue sealing around zirconia (ZrO<sub>2</sub>) abutment is critical for the long-term stability of dental implants. The goal of the study is to develop a strong basal lamina (BL)-mediated epithelial attachment to ZrO<sub>2</sub> via a novel physicochemical immobilization method. An electrophoretic fusion (EPF) method was applied to fuse a phosphonic acid (PA) linker to ZrO<sub>2</sub> discs. Bindings of the PA linker and the following protease activated receptor 4 (PAR4) were verified by Fourier-transform infrared spectroscopy (FITR). Then, ZrO<sub>2</sub> discs were doped in platelet-rich plasma (PRP). Platelet-derived growth factor (PDGF) was measured to assess platelet activation. PRP-doped discs were subsequently co-cultured with human gingival epithelial cells (OBA9) to evaluate establishment of basal lamina-mediated epithelial attachment. The EPF method achieved robust immobilization of the PA linker and PAR4 onto the ZrO<sub>2</sub> surface. The resultant PAR4-coupled ZrO<sub>2</sub> successfully induced platelet aggregation and activation. Furthermore, a BL-mediated epithelial attachment was established. The results are significant for clinical application to minimize the risk of developing peri-implant diseases. |
| first_indexed | 2024-03-09T04:02:54Z |
| format | Article |
| id | doaj.art-c9428dcbd1df4357bf80fd14223b9f19 |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-03-09T04:02:54Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-c9428dcbd1df4357bf80fd14223b9f192023-12-03T14:10:41ZengMDPI AGMaterials1996-19442022-06-011513459710.3390/ma15134597Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating PeptideChia-Yu Chen0Wonwoo Jang1David M. Kim2Masazumi Nagai3Shigemi Nagai4Department of Oral Medicine, Immunity and Infection, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USADepartment of Oral Medicine, Immunity and Infection, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USADepartment of Oral Medicine, Immunity and Infection, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USADepartment of Oral Medicine, Immunity and Infection, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USADepartment of Oral Medicine, Immunity and Infection, Harvard School of Dental Medicine, 188 Longwood Avenue, Boston, MA 02115, USASoft tissue sealing around zirconia (ZrO<sub>2</sub>) abutment is critical for the long-term stability of dental implants. The goal of the study is to develop a strong basal lamina (BL)-mediated epithelial attachment to ZrO<sub>2</sub> via a novel physicochemical immobilization method. An electrophoretic fusion (EPF) method was applied to fuse a phosphonic acid (PA) linker to ZrO<sub>2</sub> discs. Bindings of the PA linker and the following protease activated receptor 4 (PAR4) were verified by Fourier-transform infrared spectroscopy (FITR). Then, ZrO<sub>2</sub> discs were doped in platelet-rich plasma (PRP). Platelet-derived growth factor (PDGF) was measured to assess platelet activation. PRP-doped discs were subsequently co-cultured with human gingival epithelial cells (OBA9) to evaluate establishment of basal lamina-mediated epithelial attachment. The EPF method achieved robust immobilization of the PA linker and PAR4 onto the ZrO<sub>2</sub> surface. The resultant PAR4-coupled ZrO<sub>2</sub> successfully induced platelet aggregation and activation. Furthermore, a BL-mediated epithelial attachment was established. The results are significant for clinical application to minimize the risk of developing peri-implant diseases.https://www.mdpi.com/1996-1944/15/13/4597implant surfacezirconiaepithelial attachmentsoft tissue sealing |
| spellingShingle | Chia-Yu Chen Wonwoo Jang David M. Kim Masazumi Nagai Shigemi Nagai Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating Peptide Materials implant surface zirconia epithelial attachment soft tissue sealing |
| title | Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating Peptide |
| title_full | Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating Peptide |
| title_fullStr | Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating Peptide |
| title_full_unstemmed | Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating Peptide |
| title_short | Establishment of Biomimetic Soft Tissue Integration with the Surface of Zirconia Fused with Platelet-Activating Peptide |
| title_sort | establishment of biomimetic soft tissue integration with the surface of zirconia fused with platelet activating peptide |
| topic | implant surface zirconia epithelial attachment soft tissue sealing |
| url | https://www.mdpi.com/1996-1944/15/13/4597 |
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