Early and Effective Diagnosis of Sepsis Using Flow Cytometry
Objective Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable....
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2023-06-01
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| Series: | Journal of Laboratory Physicians |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1757722 |
| _version_ | 1797804910818885632 |
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| author | Pratima Verma Anurag Singh Rashmi Kushwaha Geeta Yadav Shailendra P. Verma Uma S. Singh Himanshu D. Reddy Avinash Agarwal |
| author_facet | Pratima Verma Anurag Singh Rashmi Kushwaha Geeta Yadav Shailendra P. Verma Uma S. Singh Himanshu D. Reddy Avinash Agarwal |
| author_sort | Pratima Verma |
| collection | DOAJ |
| description | Objective Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According to recent studies, neutrophil CD64 expression might be a sensitive and specific option for assessing sepsis. This study aimed to evaluate the diagnostic performance of a flow cytometry analysis for the expression of neutrophil CD64 in sepsis and its comparison with other standard tests in a tertiary care center.
Materials and Methods Prospective analysis on 40 blood samples from suspected sepsis patients admitted to intensive care units with criteria for the systemic inflammatory response syndrome on presentation was performed for expression of neutrophil CD64, C-reactive protein, procalcitonin, and complete blood count. Ten healthy volunteers were also enrolled in this prospective study. The laboratory results were compared in different groups.
Results The neutrophil CD64 had the highest diagnostic value to differentiate between patients of sepsis and nonsepsis groups with a sensitivity of 100% (95% confidence interval [CI]: 77.19–100%) and 100% (95% CI: 55.32–86.83%); specificity of 90.00% (95% CI: 59.58–99.49%) and 87.24% (95% CI: 66.69–99.61%); and likelihood ratio of 10.00 and 7.84, respectively.
Conclusion The neutrophil CD64 expression provides a more sensitive, specific, and novel marker for the early detection of sepsis in critically ill patients. |
| first_indexed | 2024-03-13T05:43:06Z |
| format | Article |
| id | doaj.art-c94375a7481240d09d6a67e24506c36b |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-03-13T05:43:06Z |
| publishDate | 2023-06-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-c94375a7481240d09d6a67e24506c36b2023-06-13T23:22:38ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262023-06-01150223023610.1055/s-0042-1757722Early and Effective Diagnosis of Sepsis Using Flow CytometryPratima Verma0Anurag Singh1Rashmi Kushwaha2Geeta Yadav3Shailendra P. Verma4Uma S. Singh5Himanshu D. Reddy6Avinash Agarwal7Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, IndiaDepartment of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, IndiaDepartment of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, IndiaDepartment of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, IndiaDepartment of Clinical Hematology, King George's Medical University, Lucknow, Uttar Pradesh, IndiaDepartment of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, IndiaDepartment of Medicine, King George's Medical University, Lucknow, Uttar Pradesh, IndiaDepartment of Critical Care Medicine, King George's Medical University, Lucknow, Uttar Pradesh, IndiaObjective Sepsis is a major global health issue due to its high death and morbidity rates. To avoid the negative effects of sepsis and decrease mortality, it is vital to diagnose and treat it as soon as possible. Blood cultures can take up to 2 days to give result, and they are not always reliable. According to recent studies, neutrophil CD64 expression might be a sensitive and specific option for assessing sepsis. This study aimed to evaluate the diagnostic performance of a flow cytometry analysis for the expression of neutrophil CD64 in sepsis and its comparison with other standard tests in a tertiary care center. Materials and Methods Prospective analysis on 40 blood samples from suspected sepsis patients admitted to intensive care units with criteria for the systemic inflammatory response syndrome on presentation was performed for expression of neutrophil CD64, C-reactive protein, procalcitonin, and complete blood count. Ten healthy volunteers were also enrolled in this prospective study. The laboratory results were compared in different groups. Results The neutrophil CD64 had the highest diagnostic value to differentiate between patients of sepsis and nonsepsis groups with a sensitivity of 100% (95% confidence interval [CI]: 77.19–100%) and 100% (95% CI: 55.32–86.83%); specificity of 90.00% (95% CI: 59.58–99.49%) and 87.24% (95% CI: 66.69–99.61%); and likelihood ratio of 10.00 and 7.84, respectively. Conclusion The neutrophil CD64 expression provides a more sensitive, specific, and novel marker for the early detection of sepsis in critically ill patients.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1757722cd64sepsisbiomarkerflow cytometryintensive care unit |
| spellingShingle | Pratima Verma Anurag Singh Rashmi Kushwaha Geeta Yadav Shailendra P. Verma Uma S. Singh Himanshu D. Reddy Avinash Agarwal Early and Effective Diagnosis of Sepsis Using Flow Cytometry Journal of Laboratory Physicians cd64 sepsis biomarker flow cytometry intensive care unit |
| title | Early and Effective Diagnosis of Sepsis Using Flow Cytometry |
| title_full | Early and Effective Diagnosis of Sepsis Using Flow Cytometry |
| title_fullStr | Early and Effective Diagnosis of Sepsis Using Flow Cytometry |
| title_full_unstemmed | Early and Effective Diagnosis of Sepsis Using Flow Cytometry |
| title_short | Early and Effective Diagnosis of Sepsis Using Flow Cytometry |
| title_sort | early and effective diagnosis of sepsis using flow cytometry |
| topic | cd64 sepsis biomarker flow cytometry intensive care unit |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0042-1757722 |
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