microRNA sequencing for biomarker detection in the diagnosis, classification and prognosis of Diffuse Large B Cell Lymphoma
Abstract Despite being considered a single disease, Diffuse Large B Cell Lymphoma (DLBCL) presents with variable backgrounds, which results in heterogeneous outcomes among patients, with 40% of them still having primary refractory disease or relapse. Thus, novel biomarkers are needed. In addition, m...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-39271-7 |
| _version_ | 1827890409188098048 |
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| author | Ane Larrabeiti-Etxebarria Nerea Bilbao-Aldaiturriaga Javier Arzuaga-Mendez Maialen Martin-Arruti Luca Cozzuto Ayman Gaafar Irune Ruiz-Diaz Isabel Guerra Idoia Martin-Guerrero Elixabet Lopez-Lopez Angela Gutierrez-Camino |
| author_facet | Ane Larrabeiti-Etxebarria Nerea Bilbao-Aldaiturriaga Javier Arzuaga-Mendez Maialen Martin-Arruti Luca Cozzuto Ayman Gaafar Irune Ruiz-Diaz Isabel Guerra Idoia Martin-Guerrero Elixabet Lopez-Lopez Angela Gutierrez-Camino |
| author_sort | Ane Larrabeiti-Etxebarria |
| collection | DOAJ |
| description | Abstract Despite being considered a single disease, Diffuse Large B Cell Lymphoma (DLBCL) presents with variable backgrounds, which results in heterogeneous outcomes among patients, with 40% of them still having primary refractory disease or relapse. Thus, novel biomarkers are needed. In addition, multiple factors regarding its pathogenesis remain unclear. In this context, recent investigations point to the relevance of microRNAs (miRNAs) in cancer. However, regarding DLBCL, there is inconsistency in the data reported. Therefore, in this work, the main goals were to determine a miRNA set with utility as biomarkers for DLBCL diagnosis, classification, prognosis and treatment response, as well as to decipher the mechanism of action of deregulated miRNAs in the origin of the disease. We analyzed miRNA expression in a cohort of 78 DLBCL patients and 17 controls using small RNA sequencing and performed a miRNA-mRNA interaction network analysis. This way, we were able to define new miRNA expression signatures for diagnosis, classification, treatment response and prognosis, and we identified plausible mechanisms of action by which deregulated miRNAs could be involved in DLBCL pathogenesis. In summary, our study remarks that miRNAs could play an important role in DLBCL. |
| first_indexed | 2024-03-12T21:10:22Z |
| format | Article |
| id | doaj.art-c945d1ea8d3b4281bcbf8570330e96aa |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-12T21:10:22Z |
| publishDate | 2023-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-c945d1ea8d3b4281bcbf8570330e96aa2023-07-30T11:15:40ZengNature PortfolioScientific Reports2045-23222023-07-0113111010.1038/s41598-023-39271-7microRNA sequencing for biomarker detection in the diagnosis, classification and prognosis of Diffuse Large B Cell LymphomaAne Larrabeiti-Etxebarria0Nerea Bilbao-Aldaiturriaga1Javier Arzuaga-Mendez2Maialen Martin-Arruti3Luca Cozzuto4Ayman Gaafar5Irune Ruiz-Diaz6Isabel Guerra7Idoia Martin-Guerrero8Elixabet Lopez-Lopez9Angela Gutierrez-Camino10Department of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country, UPV/EHUDepartment of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country, UPV/EHUDepartment of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country, UPV/EHUPathology Department, Donostia University HospitalCentre for Genomic Regulation (CRG), The Barcelona Institute of Science and TechnologyPathology Department, Cruces University HospitalPathology Department, Donostia University HospitalPathology Department, Araba University HospitalDepartment of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country, UPV/EHUDepartment of Biochemistry and Molecular Biology, Faculty of Science and Technology, University of the Basque Country, UPV/EHUPediatric Oncology Group, BioCruces Bizkaia Health Research InstituteAbstract Despite being considered a single disease, Diffuse Large B Cell Lymphoma (DLBCL) presents with variable backgrounds, which results in heterogeneous outcomes among patients, with 40% of them still having primary refractory disease or relapse. Thus, novel biomarkers are needed. In addition, multiple factors regarding its pathogenesis remain unclear. In this context, recent investigations point to the relevance of microRNAs (miRNAs) in cancer. However, regarding DLBCL, there is inconsistency in the data reported. Therefore, in this work, the main goals were to determine a miRNA set with utility as biomarkers for DLBCL diagnosis, classification, prognosis and treatment response, as well as to decipher the mechanism of action of deregulated miRNAs in the origin of the disease. We analyzed miRNA expression in a cohort of 78 DLBCL patients and 17 controls using small RNA sequencing and performed a miRNA-mRNA interaction network analysis. This way, we were able to define new miRNA expression signatures for diagnosis, classification, treatment response and prognosis, and we identified plausible mechanisms of action by which deregulated miRNAs could be involved in DLBCL pathogenesis. In summary, our study remarks that miRNAs could play an important role in DLBCL.https://doi.org/10.1038/s41598-023-39271-7 |
| spellingShingle | Ane Larrabeiti-Etxebarria Nerea Bilbao-Aldaiturriaga Javier Arzuaga-Mendez Maialen Martin-Arruti Luca Cozzuto Ayman Gaafar Irune Ruiz-Diaz Isabel Guerra Idoia Martin-Guerrero Elixabet Lopez-Lopez Angela Gutierrez-Camino microRNA sequencing for biomarker detection in the diagnosis, classification and prognosis of Diffuse Large B Cell Lymphoma Scientific Reports |
| title | microRNA sequencing for biomarker detection in the diagnosis, classification and prognosis of Diffuse Large B Cell Lymphoma |
| title_full | microRNA sequencing for biomarker detection in the diagnosis, classification and prognosis of Diffuse Large B Cell Lymphoma |
| title_fullStr | microRNA sequencing for biomarker detection in the diagnosis, classification and prognosis of Diffuse Large B Cell Lymphoma |
| title_full_unstemmed | microRNA sequencing for biomarker detection in the diagnosis, classification and prognosis of Diffuse Large B Cell Lymphoma |
| title_short | microRNA sequencing for biomarker detection in the diagnosis, classification and prognosis of Diffuse Large B Cell Lymphoma |
| title_sort | microrna sequencing for biomarker detection in the diagnosis classification and prognosis of diffuse large b cell lymphoma |
| url | https://doi.org/10.1038/s41598-023-39271-7 |
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