PI3K-mediated glioprotective effect of epidermal growth factor under oxidative stress conditions
<b>AIM:</b>To determine the effects of epidermal growth factor (EGF) on the proliferation and migration of Müller cell line Moorfields/Institute of Ophthalmology-Müller 1 (MIO-M1), and its related molecular mechanisms under normal and oxidative stress conditions.<b>METHODS:</b&g...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2014-06-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067651/ |
| _version_ | 1819141530838892544 |
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| author | Zhi-Xiang Hu Chun-Li Chen Jia-Song Yang Zhong-Lou Zhou Zong-Ming Song Zhao-Yang Wang |
| author_facet | Zhi-Xiang Hu Chun-Li Chen Jia-Song Yang Zhong-Lou Zhou Zong-Ming Song Zhao-Yang Wang |
| author_sort | Zhi-Xiang Hu |
| collection | DOAJ |
| description | <b>AIM:</b>To determine the effects of epidermal growth factor (EGF) on the proliferation and migration of Müller cell line Moorfields/Institute of Ophthalmology-Müller 1 (MIO-M1), and its related molecular mechanisms under normal and oxidative stress conditions.<b>METHODS:</b> Müller cells were cultured with different concentrations of EGF in the presence or absence of varied amounts of H2O2 and glucose oxidase (GO) which induced oxidative stress. The proliferation and migration of Müller cells were examined by 5-Bromo-2-deoxyUridine (BrdU), MTT assay, Transwell assay and scratch wound healing assays. The cell viability was determined with the MTT assay. The secretion of EGF by Müller cells was evaluated by ELISA. Western blot was performed to detect the activation of extracellular regulated protein kinases (ERK)1/2 and Akt signal pathways.<b>RESULTS:</b>EGF stimulated the proliferation and migration of Müller cells in a concentration-dependent manner <i>in vitro</i>. Underoxidative damage condition,2h of pretreatment with 10-100 ng/mL EGF can mostly inhibit 50% lethal dose of 0.08 mmol/L H2O2-induced cell damage. The Western blot results showed that after Müller cells were exposed to varying EGF for 24h, Akt and ERK1/2 were phosphorylated in a dose-dependent manner. In the presence of the LY294002, the potent PI3K inhibitor, the p-Akt was significantly attenuated.<b>CONCLUSION:</b>EGF may induce the proliferation and migration of human Müller cells through the Akt and the ERK1/2 signal pathways, and induce PI3K-mediated glioprotective effect under oxidative stress. |
| first_indexed | 2024-12-22T11:55:55Z |
| format | Article |
| id | doaj.art-c9460ded710d47968832428c3480881b |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-22T11:55:55Z |
| publishDate | 2014-06-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-c9460ded710d47968832428c3480881b2022-12-21T18:26:47ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982014-06-017341342010.3980/j.issn.2222-3959.2014.03.05PI3K-mediated glioprotective effect of epidermal growth factor under oxidative stress conditionsZhi-Xiang Hu0Chun-Li Chen1Jia-Song Yang2Zhong-Lou Zhou3Zong-Ming Song4Zhao-Yang Wang5School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China<br>School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China<br>School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China<br>School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China<br>School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China<br>Department of Ophthalmology, Zhejiang University School of Medicine, Hangzhou 310058, Zhejiang Province, China<b>AIM:</b>To determine the effects of epidermal growth factor (EGF) on the proliferation and migration of Müller cell line Moorfields/Institute of Ophthalmology-Müller 1 (MIO-M1), and its related molecular mechanisms under normal and oxidative stress conditions.<b>METHODS:</b> Müller cells were cultured with different concentrations of EGF in the presence or absence of varied amounts of H2O2 and glucose oxidase (GO) which induced oxidative stress. The proliferation and migration of Müller cells were examined by 5-Bromo-2-deoxyUridine (BrdU), MTT assay, Transwell assay and scratch wound healing assays. The cell viability was determined with the MTT assay. The secretion of EGF by Müller cells was evaluated by ELISA. Western blot was performed to detect the activation of extracellular regulated protein kinases (ERK)1/2 and Akt signal pathways.<b>RESULTS:</b>EGF stimulated the proliferation and migration of Müller cells in a concentration-dependent manner <i>in vitro</i>. Underoxidative damage condition,2h of pretreatment with 10-100 ng/mL EGF can mostly inhibit 50% lethal dose of 0.08 mmol/L H2O2-induced cell damage. The Western blot results showed that after Müller cells were exposed to varying EGF for 24h, Akt and ERK1/2 were phosphorylated in a dose-dependent manner. In the presence of the LY294002, the potent PI3K inhibitor, the p-Akt was significantly attenuated.<b>CONCLUSION:</b>EGF may induce the proliferation and migration of human Müller cells through the Akt and the ERK1/2 signal pathways, and induce PI3K-mediated glioprotective effect under oxidative stress.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067651/epidermal growth factorhuman Müller cellsPI3KPI3K |
| spellingShingle | Zhi-Xiang Hu Chun-Li Chen Jia-Song Yang Zhong-Lou Zhou Zong-Ming Song Zhao-Yang Wang PI3K-mediated glioprotective effect of epidermal growth factor under oxidative stress conditions International Journal of Ophthalmology epidermal growth factor human Müller cells PI3K PI3K |
| title | PI3K-mediated glioprotective effect of epidermal growth factor under oxidative stress conditions |
| title_full | PI3K-mediated glioprotective effect of epidermal growth factor under oxidative stress conditions |
| title_fullStr | PI3K-mediated glioprotective effect of epidermal growth factor under oxidative stress conditions |
| title_full_unstemmed | PI3K-mediated glioprotective effect of epidermal growth factor under oxidative stress conditions |
| title_short | PI3K-mediated glioprotective effect of epidermal growth factor under oxidative stress conditions |
| title_sort | pi3k mediated glioprotective effect of epidermal growth factor under oxidative stress conditions |
| topic | epidermal growth factor human Müller cells PI3K PI3K |
| url | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4067651/ |
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