Enhanced Stability of Long-Living Immobilized Recombinant β-<span style="font-variant: small-caps">d</span>-<i>N</i>-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical Applications
β-<span style="font-variant: small-caps;">d</span>-<i>N</i>-acetyl-hexosaminidase (Hex, EC 3.2.1.52) is an acid hydrolase that catalyzes the cleavage of the β-1,4 bond in <i>N</i>-acetyl-<span style="font-variant: small-caps;">d</span&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/12/2/32 |
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| author | Eleonora Calzoni Alessio Cesaretti Nicolò Montegiove Alessandro Di Michele Carla Emiliani |
| author_facet | Eleonora Calzoni Alessio Cesaretti Nicolò Montegiove Alessandro Di Michele Carla Emiliani |
| author_sort | Eleonora Calzoni |
| collection | DOAJ |
| description | β-<span style="font-variant: small-caps;">d</span>-<i>N</i>-acetyl-hexosaminidase (Hex, EC 3.2.1.52) is an acid hydrolase that catalyzes the cleavage of the β-1,4 bond in <i>N</i>-acetyl-<span style="font-variant: small-caps;">d</span>-galactosamine (Gal-NAc) and <i>N</i>-acetyl-<span style="font-variant: small-caps;">d</span>-glucosamine (Glc-NAc) from the non-reducing end of oligosaccharides and glycoconjugates. It is widely expressed in both the prokaryotic and eukaryotic world, where it performs multiple and important functions. Hex has antifungal activity in plants, is capable of degrading many biological substrates, and can play an important role in the biomedical field for the treatment of Tay-Sachs and Sandhoff diseases. With the aim being able to obtain a device with a stable enzyme, a method of covalent immobilization on polylactic acid (PLA) films was developed for the A isoform of the β-<span style="font-variant: small-caps;">d</span>-<i>N</i>-acetyl-hexosaminidase enzyme (HexA), produced in a recombinant way from Human Embryonic Kidney-293 (HEK-293) cells and suitably purified. An in-depth biochemical characterization of the immobilized enzyme was carried out, evaluating the optimal temperature, thermal stability, pH parameters, and Km value. Moreover, the stability of the enzymatic activity over time was assessed. The results obtained showed an improvement in terms of kinetic parameters and stability to heat for the enzyme following immobilization and the presence of HexA in two distinct immobilized forms, with an unexpected ability for one of them to maintain its functionality for a long period of time (over a year). The stability and functionality of the enzyme in its immobilized form are therefore extremely promising for potential biotechnological and biomedical applications. |
| first_indexed | 2024-03-10T11:33:06Z |
| format | Article |
| id | doaj.art-c94832f5639e4e06aa59c52d1bbbc09f |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-10T11:33:06Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-c94832f5639e4e06aa59c52d1bbbc09f2023-11-21T19:08:34ZengMDPI AGJournal of Functional Biomaterials2079-49832021-05-011223210.3390/jfb12020032Enhanced Stability of Long-Living Immobilized Recombinant β-<span style="font-variant: small-caps">d</span>-<i>N</i>-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical ApplicationsEleonora Calzoni0Alessio Cesaretti1Nicolò Montegiove2Alessandro Di Michele3Carla Emiliani4Department of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, ItalyDepartment of Physics and Geology, University of Perugia, 06123 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, 06123 Perugia, Italyβ-<span style="font-variant: small-caps;">d</span>-<i>N</i>-acetyl-hexosaminidase (Hex, EC 3.2.1.52) is an acid hydrolase that catalyzes the cleavage of the β-1,4 bond in <i>N</i>-acetyl-<span style="font-variant: small-caps;">d</span>-galactosamine (Gal-NAc) and <i>N</i>-acetyl-<span style="font-variant: small-caps;">d</span>-glucosamine (Glc-NAc) from the non-reducing end of oligosaccharides and glycoconjugates. It is widely expressed in both the prokaryotic and eukaryotic world, where it performs multiple and important functions. Hex has antifungal activity in plants, is capable of degrading many biological substrates, and can play an important role in the biomedical field for the treatment of Tay-Sachs and Sandhoff diseases. With the aim being able to obtain a device with a stable enzyme, a method of covalent immobilization on polylactic acid (PLA) films was developed for the A isoform of the β-<span style="font-variant: small-caps;">d</span>-<i>N</i>-acetyl-hexosaminidase enzyme (HexA), produced in a recombinant way from Human Embryonic Kidney-293 (HEK-293) cells and suitably purified. An in-depth biochemical characterization of the immobilized enzyme was carried out, evaluating the optimal temperature, thermal stability, pH parameters, and Km value. Moreover, the stability of the enzymatic activity over time was assessed. The results obtained showed an improvement in terms of kinetic parameters and stability to heat for the enzyme following immobilization and the presence of HexA in two distinct immobilized forms, with an unexpected ability for one of them to maintain its functionality for a long period of time (over a year). The stability and functionality of the enzyme in its immobilized form are therefore extremely promising for potential biotechnological and biomedical applications.https://www.mdpi.com/2079-4983/12/2/32enzyme immobilizationbiopolymersenzyme stabilityenzyme recyclabilitybiocatalysislysosomal storage diseases |
| spellingShingle | Eleonora Calzoni Alessio Cesaretti Nicolò Montegiove Alessandro Di Michele Carla Emiliani Enhanced Stability of Long-Living Immobilized Recombinant β-<span style="font-variant: small-caps">d</span>-<i>N</i>-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical Applications Journal of Functional Biomaterials enzyme immobilization biopolymers enzyme stability enzyme recyclability biocatalysis lysosomal storage diseases |
| title | Enhanced Stability of Long-Living Immobilized Recombinant β-<span style="font-variant: small-caps">d</span>-<i>N</i>-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical Applications |
| title_full | Enhanced Stability of Long-Living Immobilized Recombinant β-<span style="font-variant: small-caps">d</span>-<i>N</i>-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical Applications |
| title_fullStr | Enhanced Stability of Long-Living Immobilized Recombinant β-<span style="font-variant: small-caps">d</span>-<i>N</i>-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical Applications |
| title_full_unstemmed | Enhanced Stability of Long-Living Immobilized Recombinant β-<span style="font-variant: small-caps">d</span>-<i>N</i>-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical Applications |
| title_short | Enhanced Stability of Long-Living Immobilized Recombinant β-<span style="font-variant: small-caps">d</span>-<i>N</i>-Acetyl-Hexosaminidase A on Polylactic Acid (PLA) Films for Potential Biomedical Applications |
| title_sort | enhanced stability of long living immobilized recombinant β span style font variant small caps d span i n i acetyl hexosaminidase a on polylactic acid pla films for potential biomedical applications |
| topic | enzyme immobilization biopolymers enzyme stability enzyme recyclability biocatalysis lysosomal storage diseases |
| url | https://www.mdpi.com/2079-4983/12/2/32 |
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