A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum
Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizo-bium loti functions as an acetyl transferase. We show that the NolL-depend...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2000-04-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.475 |
| _version_ | 1828264543270207488 |
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| author | Cristina Pacios Bras Meritxell Alberich Jordá André H. M. Wijfjes Marga Harteveld Nico Stuurman Jane E. Thomas-Oates Herman P. Spaink |
| author_facet | Cristina Pacios Bras Meritxell Alberich Jordá André H. M. Wijfjes Marga Harteveld Nico Stuurman Jane E. Thomas-Oates Herman P. Spaink |
| author_sort | Cristina Pacios Bras |
| collection | DOAJ |
| description | Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizo-bium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-α-(2-aminoethoxyvinyl) glycine (AVG). |
| first_indexed | 2024-04-13T04:26:10Z |
| format | Article |
| id | doaj.art-c94e53cc228445f6b7f86a5c3163d99c |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T04:26:10Z |
| publishDate | 2000-04-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-c94e53cc228445f6b7f86a5c3163d99c2022-12-22T03:02:30ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062000-04-0113447547910.1094/MPMI.2000.13.4.475A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarumCristina Pacios BrasMeritxell Alberich JordáAndré H. M. WijfjesMarga HarteveldNico StuurmanJane E. Thomas-OatesHerman P. SpainkHeterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizo-bium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-α-(2-aminoethoxyvinyl) glycine (AVG).https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.475acetyl fucosemass spectrometryNod factors |
| spellingShingle | Cristina Pacios Bras Meritxell Alberich Jordá André H. M. Wijfjes Marga Harteveld Nico Stuurman Jane E. Thomas-Oates Herman P. Spaink A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum Molecular Plant-Microbe Interactions acetyl fucose mass spectrometry Nod factors |
| title | A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum |
| title_full | A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum |
| title_fullStr | A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum |
| title_full_unstemmed | A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum |
| title_short | A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum |
| title_sort | lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase nodz and the acetyl transferase noll in rhizobium leguminosarum |
| topic | acetyl fucose mass spectrometry Nod factors |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2000.13.4.475 |
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