ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERS
A special group of microorganisms, which are called ammonia oxidizing bacteria (AOB), is capable to reduce ammonia-nitrogen from the ecosystem. However, this bacterium is slow growing and has a low yield capacity. Hence, the objective of this research was to grow the AOB using a support medium in th...
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| Format: | Article |
| Language: | English |
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Taylor's University
2018-06-01
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| Series: | Journal of Engineering Science and Technology |
| Subjects: | |
| Online Access: | http://jestec.taylors.edu.my/Vol%2013%20issue%206%20June%202018/13_6_13.pdf |
| _version_ | 1819043320265965568 |
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| author | NORHAFEZAH KASMURI ROBERT W. LOVITT |
| author_facet | NORHAFEZAH KASMURI ROBERT W. LOVITT |
| author_sort | NORHAFEZAH KASMURI |
| collection | DOAJ |
| description | A special group of microorganisms, which are called ammonia oxidizing bacteria (AOB), is capable to reduce ammonia-nitrogen from the ecosystem. However, this bacterium is slow growing and has a low yield capacity. Hence, the objective of this research was to grow the AOB using a support medium in the form of biofilters to reduce the ammonia-nitrogen. The enrichment cultures of this AOB were performed from the inoculum of common soil and effluent from fishpond. The experiment was conducted using two sets of flasks labelled Medium A and Medium B, respectively. Each medium contained three flasks of which Flasks 1 and 2 were inoculated with the common soil and effluent from a fishpond. Flask 3 was the control, which contained neither soil nor effluent from any fishpond or bio-filter. Then the ammonia-nitrogen and the formation of nitrite-nitrogen were measured. The results showed that the enrichment culture of the effluent of the fishpond had achieved a high formation of nitrite-nitrogen (450 µg/L) in comparison with the enrichment culture of the soil (360 µg/L). A high reduction of ammonia-nitrogen (50%) was obtained from the fishpond culture rather than the soil (40%). Secondly, a higher reduction of ammonia-nitrogen and production of nitrite-nitrogen were found in the bio-filter flasks against the controls (without bio-filter). In summary, it can be concluded that the bio-filter can be a reliable support to grow AOB. |
| first_indexed | 2024-12-21T09:54:54Z |
| format | Article |
| id | doaj.art-c94e6384c27f48e6835b590dc6ede33a |
| institution | Directory Open Access Journal |
| issn | 1823-4690 |
| language | English |
| last_indexed | 2024-12-21T09:54:54Z |
| publishDate | 2018-06-01 |
| publisher | Taylor's University |
| record_format | Article |
| series | Journal of Engineering Science and Technology |
| spelling | doaj.art-c94e6384c27f48e6835b590dc6ede33a2022-12-21T19:08:06ZengTaylor's UniversityJournal of Engineering Science and Technology1823-46902018-06-0113615621572ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERSNORHAFEZAH KASMURI0ROBERT W. LOVITT1Faculty of Civil Engineering, Universiti Teknologi MARA, 40450 Shah Alam, Selangor, MalaysiaCollege of Engineering, Swansea University, Singleton Park, Swansea SA2 8PP United KingdomA special group of microorganisms, which are called ammonia oxidizing bacteria (AOB), is capable to reduce ammonia-nitrogen from the ecosystem. However, this bacterium is slow growing and has a low yield capacity. Hence, the objective of this research was to grow the AOB using a support medium in the form of biofilters to reduce the ammonia-nitrogen. The enrichment cultures of this AOB were performed from the inoculum of common soil and effluent from fishpond. The experiment was conducted using two sets of flasks labelled Medium A and Medium B, respectively. Each medium contained three flasks of which Flasks 1 and 2 were inoculated with the common soil and effluent from a fishpond. Flask 3 was the control, which contained neither soil nor effluent from any fishpond or bio-filter. Then the ammonia-nitrogen and the formation of nitrite-nitrogen were measured. The results showed that the enrichment culture of the effluent of the fishpond had achieved a high formation of nitrite-nitrogen (450 µg/L) in comparison with the enrichment culture of the soil (360 µg/L). A high reduction of ammonia-nitrogen (50%) was obtained from the fishpond culture rather than the soil (40%). Secondly, a higher reduction of ammonia-nitrogen and production of nitrite-nitrogen were found in the bio-filter flasks against the controls (without bio-filter). In summary, it can be concluded that the bio-filter can be a reliable support to grow AOB.http://jestec.taylors.edu.my/Vol%2013%20issue%206%20June%202018/13_6_13.pdfAmmonia-oxidizing bacteria (AOB)Batch cultureBio-filterEffluent from fishpondSoil |
| spellingShingle | NORHAFEZAH KASMURI ROBERT W. LOVITT ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERS Journal of Engineering Science and Technology Ammonia-oxidizing bacteria (AOB) Batch culture Bio-filter Effluent from fishpond Soil |
| title | ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERS |
| title_full | ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERS |
| title_fullStr | ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERS |
| title_full_unstemmed | ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERS |
| title_short | ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERS |
| title_sort | enriching the culture of ammonia oxidizing bacteria from soil and fishpond with bio filters |
| topic | Ammonia-oxidizing bacteria (AOB) Batch culture Bio-filter Effluent from fishpond Soil |
| url | http://jestec.taylors.edu.my/Vol%2013%20issue%206%20June%202018/13_6_13.pdf |
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