Top-down Nanoscavengers for the protection of organophosphate-challenged cells
Background: Organophosphates (OPs) can directly poison various types of mammalian cells, only with several awkward bottom-up antagonists. A top-down nanoscavenger was developed to radically and etiologically prevent cell damage, while insufficient subcellular or molecular blocking mechanisms were ex...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Giant |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666542523000759 |
| _version_ | 1797248300946030592 |
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| author | Shuaijun Zou Qianqian Wang Juxingsi Song Guoyan Liu Fuhai Zhang Jie Li Fan Wang Yanan Hu Yongfei Lv Dayuan Zhou Qian He Beilei Wang Liming Zhang |
| author_facet | Shuaijun Zou Qianqian Wang Juxingsi Song Guoyan Liu Fuhai Zhang Jie Li Fan Wang Yanan Hu Yongfei Lv Dayuan Zhou Qian He Beilei Wang Liming Zhang |
| author_sort | Shuaijun Zou |
| collection | DOAJ |
| description | Background: Organophosphates (OPs) can directly poison various types of mammalian cells, only with several awkward bottom-up antagonists. A top-down nanoscavenger was developed to radically and etiologically prevent cell damage, while insufficient subcellular or molecular blocking mechanisms were explored. Methods: The top-down nanoscavengers were first developed and carefully characterized. EL4 cells were then adopted as model cells to clarify the protective effects and mechanisms of nanoscavengers. After detecting the cell viability and apoptosis, oxidative stress and mitochondrial injury markers, DNA damage index as well as relevant apoptotic factors were sequentially examined to clarify what damage the top-down nanoscavengers inhibited. Results: The well-characterized nanoscavengers exerted a top-down and etiological protective effects on EL4 cell viability via radically eliminating OPs and blocking OP-cell interactions. As a result, the intracellular oxidative stress-related markers including ROS production and abnormal antioxidants, mitochondrial injuries as well as DNA damage were inhibited. Accordingly, above damage-induced apoptosis was also blocked. Conclusion: In conclusion, this study clarified the protective mechanisms of a novel enzyme-based nanoscavengers for OP-challenged cells and provided a potent antidote for OP poisoning. |
| first_indexed | 2024-03-09T07:33:35Z |
| format | Article |
| id | doaj.art-c951fa3344224f899fdab16d410048fb |
| institution | Directory Open Access Journal |
| issn | 2666-5425 |
| language | English |
| last_indexed | 2024-04-24T20:12:24Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Giant |
| spelling | doaj.art-c951fa3344224f899fdab16d410048fb2024-03-23T06:26:03ZengElsevierGiant2666-54252024-03-0117100213Top-down Nanoscavengers for the protection of organophosphate-challenged cellsShuaijun Zou0Qianqian Wang1Juxingsi Song2Guoyan Liu3Fuhai Zhang4Jie Li5Fan Wang6Yanan Hu7Yongfei Lv8Dayuan Zhou9Qian He10Beilei Wang11Liming Zhang12Department of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, China; Department of Marine Biological Injury and Dermatology, Naval Medical Centre, Naval Medical University, Shanghai 200052, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaDepartment of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, ChinaThe Third Affiliated Hospital, Naval Medical University, Shanghai 200433, China; Corresponding authors.Department of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, China; Corresponding authors.Department of Marine Biomedicine and Polar Medicine, Naval Medical Centre, Naval Medical University, Shanghai 200433, China; Corresponding authors.Background: Organophosphates (OPs) can directly poison various types of mammalian cells, only with several awkward bottom-up antagonists. A top-down nanoscavenger was developed to radically and etiologically prevent cell damage, while insufficient subcellular or molecular blocking mechanisms were explored. Methods: The top-down nanoscavengers were first developed and carefully characterized. EL4 cells were then adopted as model cells to clarify the protective effects and mechanisms of nanoscavengers. After detecting the cell viability and apoptosis, oxidative stress and mitochondrial injury markers, DNA damage index as well as relevant apoptotic factors were sequentially examined to clarify what damage the top-down nanoscavengers inhibited. Results: The well-characterized nanoscavengers exerted a top-down and etiological protective effects on EL4 cell viability via radically eliminating OPs and blocking OP-cell interactions. As a result, the intracellular oxidative stress-related markers including ROS production and abnormal antioxidants, mitochondrial injuries as well as DNA damage were inhibited. Accordingly, above damage-induced apoptosis was also blocked. Conclusion: In conclusion, this study clarified the protective mechanisms of a novel enzyme-based nanoscavengers for OP-challenged cells and provided a potent antidote for OP poisoning.http://www.sciencedirect.com/science/article/pii/S2666542523000759OrganophosphatesEnzyme immobilizationNanoenzymeBioscavengersCellular damage |
| spellingShingle | Shuaijun Zou Qianqian Wang Juxingsi Song Guoyan Liu Fuhai Zhang Jie Li Fan Wang Yanan Hu Yongfei Lv Dayuan Zhou Qian He Beilei Wang Liming Zhang Top-down Nanoscavengers for the protection of organophosphate-challenged cells Giant Organophosphates Enzyme immobilization Nanoenzyme Bioscavengers Cellular damage |
| title | Top-down Nanoscavengers for the protection of organophosphate-challenged cells |
| title_full | Top-down Nanoscavengers for the protection of organophosphate-challenged cells |
| title_fullStr | Top-down Nanoscavengers for the protection of organophosphate-challenged cells |
| title_full_unstemmed | Top-down Nanoscavengers for the protection of organophosphate-challenged cells |
| title_short | Top-down Nanoscavengers for the protection of organophosphate-challenged cells |
| title_sort | top down nanoscavengers for the protection of organophosphate challenged cells |
| topic | Organophosphates Enzyme immobilization Nanoenzyme Bioscavengers Cellular damage |
| url | http://www.sciencedirect.com/science/article/pii/S2666542523000759 |
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