Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (<i>Solanum tuberosum</i> L.) Accessions Using SSR Markers
The allelic variations in a diversity panel of 353 potato accessions, including 256 accessions belonging to <i>Solanum tuberosum</i> sub spp. <i>tuberosum</i>, 49 accessions belonging to <i>Solanum tuberosum</i> sub spp. <i>andigena</i>, and 48 Indian...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/2/294 |
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| author | Vinay Bhardwaj Ashwani Kumar Sanjeev Sharma Baljeet Singh Poonam Salej Sood Bhawna Dipta Rajender Singh Sundaresha Siddappa Ajay Kumar Thakur Dalamu Dalamu Ashwani Kumar Sharma Vinod Kumar Mehi Lal Devendra Kumar |
| author_facet | Vinay Bhardwaj Ashwani Kumar Sanjeev Sharma Baljeet Singh Poonam Salej Sood Bhawna Dipta Rajender Singh Sundaresha Siddappa Ajay Kumar Thakur Dalamu Dalamu Ashwani Kumar Sharma Vinod Kumar Mehi Lal Devendra Kumar |
| author_sort | Vinay Bhardwaj |
| collection | DOAJ |
| description | The allelic variations in a diversity panel of 353 potato accessions, including 256 accessions belonging to <i>Solanum tuberosum</i> sub spp. <i>tuberosum</i>, 49 accessions belonging to <i>Solanum tuberosum</i> sub spp. <i>andigena</i>, and 48 Indian potato varieties were analysed using 25 simple sequence repeat (SSR) markers. The SSR allelic profiles revealed high levels of polymorphism and distinctness among the accessions studied. A total of 343 alleles of 25 SSR markers were observed in the diversity panel of 353 highly diverse tetraploid potato accessions. The number of alleles produced per SSR varied from 8 for the marker STM1053 to 25 for the marker STIKA. The polymorphic information content (PIC) ranged from 0.66 (STG0010) to 0.93 (STM1106) with an average of 0.82. The cluster analysis using the SSR allelic profiles of 353 accessions divided the population into five major groups. The association mapping for late blight resistance identified six markers with the general linear model (GLM), and out of these six markers significance of three markers was reconfirmed with the mixed linear model (MLM). The findings of this study suggest that SSRs are the appropriate markers for evaluating genetic diversity and population structure within different potato germplasm collections. A significant diversity across the tetraploid potato accessions was observed. Moreover, the markers identified in this study could be useful in marker-assisted selection (MAS) breeding in potato for late blight resistance (LBR). |
| first_indexed | 2024-03-11T09:18:17Z |
| format | Article |
| id | doaj.art-c952bfe7c82145268babcd3d08416e78 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T09:18:17Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-c952bfe7c82145268babcd3d08416e782023-11-16T18:32:28ZengMDPI AGAgronomy2073-43952023-01-0113229410.3390/agronomy13020294Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (<i>Solanum tuberosum</i> L.) Accessions Using SSR MarkersVinay Bhardwaj0Ashwani Kumar1Sanjeev Sharma2Baljeet Singh3Poonam4Salej Sood5Bhawna Dipta6Rajender Singh7Sundaresha Siddappa8Ajay Kumar Thakur9Dalamu Dalamu10Ashwani Kumar Sharma11Vinod Kumar12Mehi Lal13Devendra Kumar14ICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaICAR—Central Potato Research Institute, Modipuram 250110, IndiaICAR—Central Potato Research Institute, Shimla 171001, IndiaThe allelic variations in a diversity panel of 353 potato accessions, including 256 accessions belonging to <i>Solanum tuberosum</i> sub spp. <i>tuberosum</i>, 49 accessions belonging to <i>Solanum tuberosum</i> sub spp. <i>andigena</i>, and 48 Indian potato varieties were analysed using 25 simple sequence repeat (SSR) markers. The SSR allelic profiles revealed high levels of polymorphism and distinctness among the accessions studied. A total of 343 alleles of 25 SSR markers were observed in the diversity panel of 353 highly diverse tetraploid potato accessions. The number of alleles produced per SSR varied from 8 for the marker STM1053 to 25 for the marker STIKA. The polymorphic information content (PIC) ranged from 0.66 (STG0010) to 0.93 (STM1106) with an average of 0.82. The cluster analysis using the SSR allelic profiles of 353 accessions divided the population into five major groups. The association mapping for late blight resistance identified six markers with the general linear model (GLM), and out of these six markers significance of three markers was reconfirmed with the mixed linear model (MLM). The findings of this study suggest that SSRs are the appropriate markers for evaluating genetic diversity and population structure within different potato germplasm collections. A significant diversity across the tetraploid potato accessions was observed. Moreover, the markers identified in this study could be useful in marker-assisted selection (MAS) breeding in potato for late blight resistance (LBR).https://www.mdpi.com/2073-4395/13/2/294genetic diversitypopulation structureSSR markerslate blightassociation mappingpotato |
| spellingShingle | Vinay Bhardwaj Ashwani Kumar Sanjeev Sharma Baljeet Singh Poonam Salej Sood Bhawna Dipta Rajender Singh Sundaresha Siddappa Ajay Kumar Thakur Dalamu Dalamu Ashwani Kumar Sharma Vinod Kumar Mehi Lal Devendra Kumar Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (<i>Solanum tuberosum</i> L.) Accessions Using SSR Markers Agronomy genetic diversity population structure SSR markers late blight association mapping potato |
| title | Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (<i>Solanum tuberosum</i> L.) Accessions Using SSR Markers |
| title_full | Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (<i>Solanum tuberosum</i> L.) Accessions Using SSR Markers |
| title_fullStr | Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (<i>Solanum tuberosum</i> L.) Accessions Using SSR Markers |
| title_full_unstemmed | Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (<i>Solanum tuberosum</i> L.) Accessions Using SSR Markers |
| title_short | Analysis of Genetic Diversity, Population Structure and Association Mapping for Late Blight Resistance in Potato (<i>Solanum tuberosum</i> L.) Accessions Using SSR Markers |
| title_sort | analysis of genetic diversity population structure and association mapping for late blight resistance in potato i solanum tuberosum i l accessions using ssr markers |
| topic | genetic diversity population structure SSR markers late blight association mapping potato |
| url | https://www.mdpi.com/2073-4395/13/2/294 |
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