Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern China
Ophiocordyceps contains the largest number of Cordyceps sensu lato, various species of which are of great medicinal value. In this study, a new entomopathogenic fungus, Ophiocordyceps nujiangensis, from Yunnan in southwestern China, was described using morphological, phylogenetic, and mitogenomic ev...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2022-12-01
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| Series: | MycoKeys |
| Online Access: | https://mycokeys.pensoft.net/article/89425/download/pdf/ |
| _version_ | 1797978516921253888 |
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| author | Tao Sun Weiqiu Zou Quanying Dong Ou Huang Dexiang Tang Hong Yu |
| author_facet | Tao Sun Weiqiu Zou Quanying Dong Ou Huang Dexiang Tang Hong Yu |
| author_sort | Tao Sun |
| collection | DOAJ |
| description | Ophiocordyceps contains the largest number of Cordyceps sensu lato, various species of which are of great medicinal value. In this study, a new entomopathogenic fungus, Ophiocordyceps nujiangensis, from Yunnan in southwestern China, was described using morphological, phylogenetic, and mitogenomic evidence, and its fungal community composition was identified. It was morphologically characterized by a solitary, woody, and dark brown stromata, smooth-walled and septate hyphae, solitary and gradually tapering conidiogenous cells with plenty of warty protrusions, and oval or fusiform conidia (6.4–11.2 × 3.7–6.4 µm) with mucinous sheath. The phylogenetic location of O. nujiangensis was determined based on the Bayesian inference (BI) and the maximum likelihood (ML) analyses by concatenating nrSSU, nrLSU, tef-1a, rpb1, and rpb2 datasets, and ten mitochondrial protein-coding genes (PCGs) datasets (atp6, atp9, cob, cox2, nad1, nad2, nad3, nad4, nad4L, and nad5). Phylogenetic analyses revealed that O. nujiangensis belonged to the Hirsutella sinensis subclade within the Hirsutella clade of Ophiocordyceps. And O. nujiangensis was phylogenetically clustered with O. karstii, O. liangshanensis, and O. sinensis. Simultaneously, five fungal phyla and 151 fungal genera were recognized in the analysis of the fungal community of O. nujiangensis. The fungal community composition differed from that of O. sinensis, and differences in the microbial community composition of closely related species might be appropriate as further evidence for taxonomy. |
| first_indexed | 2024-04-11T05:25:18Z |
| format | Article |
| id | doaj.art-c952e031eca0426a8ae0805e07a04d76 |
| institution | Directory Open Access Journal |
| issn | 1314-4049 |
| language | English |
| last_indexed | 2024-04-11T05:25:18Z |
| publishDate | 2022-12-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | MycoKeys |
| spelling | doaj.art-c952e031eca0426a8ae0805e07a04d762022-12-23T09:54:05ZengPensoft PublishersMycoKeys1314-40492022-12-01949110810.3897/mycokeys.94.8942589425Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern ChinaTao Sun0Weiqiu Zou1Quanying Dong2Ou Huang3Dexiang Tang4Hong Yu5Yunnan UniversityYunnan UniversityYunnan UniversityYunnan UniversityYunnan UniversitySchool of Ecology and Environmental ScienceOphiocordyceps contains the largest number of Cordyceps sensu lato, various species of which are of great medicinal value. In this study, a new entomopathogenic fungus, Ophiocordyceps nujiangensis, from Yunnan in southwestern China, was described using morphological, phylogenetic, and mitogenomic evidence, and its fungal community composition was identified. It was morphologically characterized by a solitary, woody, and dark brown stromata, smooth-walled and septate hyphae, solitary and gradually tapering conidiogenous cells with plenty of warty protrusions, and oval or fusiform conidia (6.4–11.2 × 3.7–6.4 µm) with mucinous sheath. The phylogenetic location of O. nujiangensis was determined based on the Bayesian inference (BI) and the maximum likelihood (ML) analyses by concatenating nrSSU, nrLSU, tef-1a, rpb1, and rpb2 datasets, and ten mitochondrial protein-coding genes (PCGs) datasets (atp6, atp9, cob, cox2, nad1, nad2, nad3, nad4, nad4L, and nad5). Phylogenetic analyses revealed that O. nujiangensis belonged to the Hirsutella sinensis subclade within the Hirsutella clade of Ophiocordyceps. And O. nujiangensis was phylogenetically clustered with O. karstii, O. liangshanensis, and O. sinensis. Simultaneously, five fungal phyla and 151 fungal genera were recognized in the analysis of the fungal community of O. nujiangensis. The fungal community composition differed from that of O. sinensis, and differences in the microbial community composition of closely related species might be appropriate as further evidence for taxonomy.https://mycokeys.pensoft.net/article/89425/download/pdf/ |
| spellingShingle | Tao Sun Weiqiu Zou Quanying Dong Ou Huang Dexiang Tang Hong Yu Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern China MycoKeys |
| title | Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern China |
| title_full | Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern China |
| title_fullStr | Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern China |
| title_full_unstemmed | Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern China |
| title_short | Morphology, phylogeny, mitogenomics and metagenomics reveal a new entomopathogenic fungus Ophiocordyceps nujiangensis (Hypocreales, Ophiocordycipitaceae) from Southwestern China |
| title_sort | morphology phylogeny mitogenomics and metagenomics reveal a new entomopathogenic fungus ophiocordyceps nujiangensis hypocreales ophiocordycipitaceae from southwestern china |
| url | https://mycokeys.pensoft.net/article/89425/download/pdf/ |
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