Euphorbia formosana Root Extract Induces Apoptosis by Caspase-Dependent Cell Death via Fas and Mitochondrial Pathway in THP-1 Human Leukemic Cells
Acute myeloid leukemia (AML), a very rare type of cancer, generally affects patients over 50 years old. While clinical drugs to treat advanced stages of AML exist, the disease becomes increasingly resistant to therapies. Euphorbia formosana Hayata (EF) is a native Taiwanese medicinal plant used to t...
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MDPI AG
2013-02-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/18/2/1949 |
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| author | Yann-Lii Leu Yi-Han Chiu Chin-Piao Chen Chin-Feng Wan Chih-Jui Chang Yi-Jen Hsieh Kou-Cheng Peng |
| author_facet | Yann-Lii Leu Yi-Han Chiu Chin-Piao Chen Chin-Feng Wan Chih-Jui Chang Yi-Jen Hsieh Kou-Cheng Peng |
| author_sort | Yann-Lii Leu |
| collection | DOAJ |
| description | Acute myeloid leukemia (AML), a very rare type of cancer, generally affects patients over 50 years old. While clinical drugs to treat advanced stages of AML exist, the disease becomes increasingly resistant to therapies. Euphorbia formosana Hayata (EF) is a native Taiwanese medicinal plant used to treat rheumatism, liver cirrhosis, herpes zoster, scabies, and photoaging, along with tumor suppression. However, the mechanisms by which it suppresses tumors have not been explored. Here, we provide molecular evidence that a hot-water extract of Euphorbia formosana (EFW) selectively inhibited the growth of human leukemic cancer cells more than other solid human cancer cell lines. Most importantly, the plant extract had limited toxicity toward healthy peripheral blood mononuclear cells (PBMCs). After THP-1 leukemic cells were treated with 50–100 µg/mL EFW for one day, the S phase DNA content of the cells increased, while treatment with 200–400 µg/mL caused the cells to accumulate in the G0/G1 phase. Notably, EFW did not affect A-549 lung cancer cells. The effectiveness of EFW against THP-1 cells may be through caspase-dependent apoptosis in leukemic cells, which is mediated through the Fas and mitochondrial pathways. The potent antileukemic activity of EFW in vitro warrants further investigation of this plant to treat leukemias and other malignancies. |
| first_indexed | 2024-12-10T14:07:58Z |
| format | Article |
| id | doaj.art-c953ec46405c4009b27f7c668078fad4 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T14:07:58Z |
| publishDate | 2013-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-c953ec46405c4009b27f7c668078fad42022-12-22T01:45:36ZengMDPI AGMolecules1420-30492013-02-011821949196210.3390/molecules18021949Euphorbia formosana Root Extract Induces Apoptosis by Caspase-Dependent Cell Death via Fas and Mitochondrial Pathway in THP-1 Human Leukemic CellsYann-Lii LeuYi-Han ChiuChin-Piao ChenChin-Feng WanChih-Jui ChangYi-Jen HsiehKou-Cheng PengAcute myeloid leukemia (AML), a very rare type of cancer, generally affects patients over 50 years old. While clinical drugs to treat advanced stages of AML exist, the disease becomes increasingly resistant to therapies. Euphorbia formosana Hayata (EF) is a native Taiwanese medicinal plant used to treat rheumatism, liver cirrhosis, herpes zoster, scabies, and photoaging, along with tumor suppression. However, the mechanisms by which it suppresses tumors have not been explored. Here, we provide molecular evidence that a hot-water extract of Euphorbia formosana (EFW) selectively inhibited the growth of human leukemic cancer cells more than other solid human cancer cell lines. Most importantly, the plant extract had limited toxicity toward healthy peripheral blood mononuclear cells (PBMCs). After THP-1 leukemic cells were treated with 50–100 µg/mL EFW for one day, the S phase DNA content of the cells increased, while treatment with 200–400 µg/mL caused the cells to accumulate in the G0/G1 phase. Notably, EFW did not affect A-549 lung cancer cells. The effectiveness of EFW against THP-1 cells may be through caspase-dependent apoptosis in leukemic cells, which is mediated through the Fas and mitochondrial pathways. The potent antileukemic activity of EFW in vitro warrants further investigation of this plant to treat leukemias and other malignancies.http://www.mdpi.com/1420-3049/18/2/1949Euphorbia formosanaapoptosismitochondrial pathwayFas pathway |
| spellingShingle | Yann-Lii Leu Yi-Han Chiu Chin-Piao Chen Chin-Feng Wan Chih-Jui Chang Yi-Jen Hsieh Kou-Cheng Peng Euphorbia formosana Root Extract Induces Apoptosis by Caspase-Dependent Cell Death via Fas and Mitochondrial Pathway in THP-1 Human Leukemic Cells Molecules Euphorbia formosana apoptosis mitochondrial pathway Fas pathway |
| title | Euphorbia formosana Root Extract Induces Apoptosis by Caspase-Dependent Cell Death via Fas and Mitochondrial Pathway in THP-1 Human Leukemic Cells |
| title_full | Euphorbia formosana Root Extract Induces Apoptosis by Caspase-Dependent Cell Death via Fas and Mitochondrial Pathway in THP-1 Human Leukemic Cells |
| title_fullStr | Euphorbia formosana Root Extract Induces Apoptosis by Caspase-Dependent Cell Death via Fas and Mitochondrial Pathway in THP-1 Human Leukemic Cells |
| title_full_unstemmed | Euphorbia formosana Root Extract Induces Apoptosis by Caspase-Dependent Cell Death via Fas and Mitochondrial Pathway in THP-1 Human Leukemic Cells |
| title_short | Euphorbia formosana Root Extract Induces Apoptosis by Caspase-Dependent Cell Death via Fas and Mitochondrial Pathway in THP-1 Human Leukemic Cells |
| title_sort | euphorbia formosana root extract induces apoptosis by caspase dependent cell death via fas and mitochondrial pathway in thp 1 human leukemic cells |
| topic | Euphorbia formosana apoptosis mitochondrial pathway Fas pathway |
| url | http://www.mdpi.com/1420-3049/18/2/1949 |
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