Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation
<i>Lespedeza cuneata</i>, belonging to Fabaceae, is well-known as Chinese bushclover, and it has been used in traditional folk medicines for the treatment of disorders, such as diabetes, hematuria, and insomnia. As part of continuing research projects to discover interesting natural comp...
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2021-11-01
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| author | Heesun Kang Min Jeong Yoo Sang Ah Yi Tae Wan Kim Ji Won Ha Myung Woo Na Kun Hee Park Seon-Hee Kim Jeung-Whan Han Tae Su Jang Ki Hyun Kim |
| author_facet | Heesun Kang Min Jeong Yoo Sang Ah Yi Tae Wan Kim Ji Won Ha Myung Woo Na Kun Hee Park Seon-Hee Kim Jeung-Whan Han Tae Su Jang Ki Hyun Kim |
| author_sort | Heesun Kang |
| collection | DOAJ |
| description | <i>Lespedeza cuneata</i>, belonging to Fabaceae, is well-known as Chinese bushclover, and it has been used in traditional folk medicines for the treatment of disorders, such as diabetes, hematuria, and insomnia. As part of continuing research projects to discover interesting natural compounds with biological activities from Korean medicinal plants, the phytochemical investigation of <i>L. cuneata</i> resulted in the isolation of five chemical constituents: α-tocopherol (<b>1</b>), 7a-methoxy-α-tocopherol (<b>2</b>), 13(<i>R</i>)-hydroxy-octadeca-(9<i>Z</i>,11<i>E</i>,15<i>Z</i>)-trien-oic acid (<b>3</b>), α-dimorphecolic acid (<b>4</b>), and lupeol (<b>5</b>). The structural determination of the isolated compounds was elucidated from data gathered through nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography–mass spectrometry (LC/MS). Until now, this study is the first to report these five compounds from the plant <i>L. cuneata</i>. Moreover, these isolated compounds (<b>1</b>–<b>5</b>) were evaluated for their anti-adipogenesis effects and their role in lipid metabolism during adipocyte maturation. As a result, the upregulation of mRNA expression levels of <i>Fabp4</i> from 3T3-L1 pre-adipocytes treated with compounds <b>3</b> and <b>4</b> demonstrated that these compounds efficiently induced adipocyte differentiation. Furthermore, compounds <b>3</b> and <b>4</b> were found to regulate lipid metabolism by the induction of lipolytic and of lipogenic gene expressions. Therefore, experimental data from these findings supported that the compounds <b>3</b> and <b>4</b> induce the adipogenesis of 3T3-L1 pre-adipocytes and regulate lipid metabolism. |
| first_indexed | 2024-03-10T05:04:28Z |
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| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-10T05:04:28Z |
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| spelling | doaj.art-c95539f093654e3b9d643ac619cdfdfa2023-11-23T01:29:47ZengMDPI AGSeparations2297-87392021-11-0181120310.3390/separations8110203Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte MaturationHeesun Kang0Min Jeong Yoo1Sang Ah Yi2Tae Wan Kim3Ji Won Ha4Myung Woo Na5Kun Hee Park6Seon-Hee Kim7Jeung-Whan Han8Tae Su Jang9Ki Hyun Kim10School of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaDepartment of Food Science and Biotechnology, Sungkyunkwan University, Suwon 16419, KoreaSungkyun Biotech Co., Ltd., Suwon 16419, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, KoreaCollege of Medicine, Dankook University, Cheonan 31116, KoreaSchool of Pharmacy, Sungkyunkwan University, Suwon 16419, Korea<i>Lespedeza cuneata</i>, belonging to Fabaceae, is well-known as Chinese bushclover, and it has been used in traditional folk medicines for the treatment of disorders, such as diabetes, hematuria, and insomnia. As part of continuing research projects to discover interesting natural compounds with biological activities from Korean medicinal plants, the phytochemical investigation of <i>L. cuneata</i> resulted in the isolation of five chemical constituents: α-tocopherol (<b>1</b>), 7a-methoxy-α-tocopherol (<b>2</b>), 13(<i>R</i>)-hydroxy-octadeca-(9<i>Z</i>,11<i>E</i>,15<i>Z</i>)-trien-oic acid (<b>3</b>), α-dimorphecolic acid (<b>4</b>), and lupeol (<b>5</b>). The structural determination of the isolated compounds was elucidated from data gathered through nuclear magnetic resonance (NMR) spectroscopy and liquid chromatography–mass spectrometry (LC/MS). Until now, this study is the first to report these five compounds from the plant <i>L. cuneata</i>. Moreover, these isolated compounds (<b>1</b>–<b>5</b>) were evaluated for their anti-adipogenesis effects and their role in lipid metabolism during adipocyte maturation. As a result, the upregulation of mRNA expression levels of <i>Fabp4</i> from 3T3-L1 pre-adipocytes treated with compounds <b>3</b> and <b>4</b> demonstrated that these compounds efficiently induced adipocyte differentiation. Furthermore, compounds <b>3</b> and <b>4</b> were found to regulate lipid metabolism by the induction of lipolytic and of lipogenic gene expressions. Therefore, experimental data from these findings supported that the compounds <b>3</b> and <b>4</b> induce the adipogenesis of 3T3-L1 pre-adipocytes and regulate lipid metabolism.https://www.mdpi.com/2297-8739/8/11/203<i>Lespedeza cuneata</i>Fabaceaestructural elucidation3T3-L1 pre-adipocytesadipogenesis |
| spellingShingle | Heesun Kang Min Jeong Yoo Sang Ah Yi Tae Wan Kim Ji Won Ha Myung Woo Na Kun Hee Park Seon-Hee Kim Jeung-Whan Han Tae Su Jang Ki Hyun Kim Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation Separations <i>Lespedeza cuneata</i> Fabaceae structural elucidation 3T3-L1 pre-adipocytes adipogenesis |
| title | Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation |
| title_full | Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation |
| title_fullStr | Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation |
| title_full_unstemmed | Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation |
| title_short | Phytochemical Constituents Identified from the Aerial Parts of <i>Lespedeza cuneata</i> and Their Effects on Lipid Metabolism during Adipocyte Maturation |
| title_sort | phytochemical constituents identified from the aerial parts of i lespedeza cuneata i and their effects on lipid metabolism during adipocyte maturation |
| topic | <i>Lespedeza cuneata</i> Fabaceae structural elucidation 3T3-L1 pre-adipocytes adipogenesis |
| url | https://www.mdpi.com/2297-8739/8/11/203 |
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