Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This...
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MDPI AG
2020-07-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/7/353 |
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| author | Luz Verónica Pacheco Javier Parada José Ricardo Pérez-Correa María Salomé Mariotti-Celis Fernanda Erpel Angara Zambrano Mauricio Palacios |
| author_facet | Luz Verónica Pacheco Javier Parada José Ricardo Pérez-Correa María Salomé Mariotti-Celis Fernanda Erpel Angara Zambrano Mauricio Palacios |
| author_sort | Luz Verónica Pacheco |
| collection | DOAJ |
| description | The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the <i>Durvillaea antarctica</i> (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity. |
| first_indexed | 2024-03-10T18:37:53Z |
| format | Article |
| id | doaj.art-c957d829fc2f468c91dbd5a4a4c481ee |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T18:37:53Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-c957d829fc2f468c91dbd5a4a4c481ee2023-11-20T06:08:37ZengMDPI AGMarine Drugs1660-33972020-07-0118735310.3390/md18070353Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch DigestionLuz Verónica Pacheco0Javier Parada1José Ricardo Pérez-Correa2María Salomé Mariotti-Celis3Fernanda Erpel4Angara Zambrano5Mauricio Palacios6Graduate School, Faculty of Agricultural Sciences, Universidad Austral de Chile, Valdivia 5090000, ChileInstitute of Food Science and Technology, Faculty of Agricultural Sciences, Universidad Austral de Chile, Valdivia 5090000, ChileDepartment of Chemical and Bioprocess Engineering, Pontificia Universidad Católica de Chile, Macul, Santiago 7810000, ChilePrograma Institucional de Fomento a la Investigación, Desarrollo e Innovación, Universidad Tecnológica Metropolitana, Santiago 8940577, ChileDepartment of Chemical and Bioprocess Engineering, Pontificia Universidad Católica de Chile, Macul, Santiago 7810000, ChileInstituto de Bioquímica y Microbiología, Facultad de Ciencias, Universidad Austral de Chile, Valdivia 5090000, ChileCentro FONDAP de Investigación Dinámica de Ecosistemas Marinos de Altas Latitudes (IDEAL), Valdivia 5090000, ChileThe increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the <i>Durvillaea antarctica</i> (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity.https://www.mdpi.com/1660-3397/18/7/353seaweed polyphenolshypoglycemic effectstarch digestionenzyme inhibitioncochayuyo |
| spellingShingle | Luz Verónica Pacheco Javier Parada José Ricardo Pérez-Correa María Salomé Mariotti-Celis Fernanda Erpel Angara Zambrano Mauricio Palacios Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion Marine Drugs seaweed polyphenols hypoglycemic effect starch digestion enzyme inhibition cochayuyo |
| title | Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
| title_full | Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
| title_fullStr | Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
| title_full_unstemmed | Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
| title_short | Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
| title_sort | bioactive polyphenols from southern chile seaweed as inhibitors of enzymes for starch digestion |
| topic | seaweed polyphenols hypoglycemic effect starch digestion enzyme inhibition cochayuyo |
| url | https://www.mdpi.com/1660-3397/18/7/353 |
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