Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach
Lumpy skin disease (LSD) is a contagious disease among cattle and buffalo worldwide. Currently, an enzyme-linked immunosorbent assay (ELISA) has been recognized as an efficient diagnostic tool that is less time-consuming and easier than the viral neutralization test to measure the antibody levels. I...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023007065 |
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| author | Nattawooti Sthitmatee Pallop Tankaew Wittawat Modethed Amarin Rittipornlertrak Anucha Muenthaisong Nisachon Apinda Pongpisid Koonyosying Boondarika Nambooppha Paweena Chomjit Kanokwan Sangkakam Tawatchai Singhla Paramintra Vinitchaikul Kittikorn Boonsri Kidsadagon Pringproa Veerasak Punyapornwithaya Khwanchai Kreausukon |
| author_facet | Nattawooti Sthitmatee Pallop Tankaew Wittawat Modethed Amarin Rittipornlertrak Anucha Muenthaisong Nisachon Apinda Pongpisid Koonyosying Boondarika Nambooppha Paweena Chomjit Kanokwan Sangkakam Tawatchai Singhla Paramintra Vinitchaikul Kittikorn Boonsri Kidsadagon Pringproa Veerasak Punyapornwithaya Khwanchai Kreausukon |
| author_sort | Nattawooti Sthitmatee |
| collection | DOAJ |
| description | Lumpy skin disease (LSD) is a contagious disease among cattle and buffalo worldwide. Currently, an enzyme-linked immunosorbent assay (ELISA) has been recognized as an efficient diagnostic tool that is less time-consuming and easier than the viral neutralization test to measure the antibody levels. In the present study, an in-house method of indirect ELISA was developed to detect the bovine antibodies against Lumpy skin disease virus (LSDV) and its performance was assessed using field samples. This in-house method has been compared with the commercial ELISA test kit for detection of bovine antibodies against LSDV. The sensitivity (Se) and the specificity (Sp) of the test were estimated using a Bayesian latent class model. Checkerboard titration was performed using the naturally LSDV-infected bovine sera and colostrum-deprived calf sera. The LSDV antigen concentrations (1 TCID50/mL), the sample serum (1:500), and goat anti-bovine immunoglobulin G (IgG) labeled with horseradish peroxidase (HRP) (1:10,000) were determined to be optimal for this assay. The calculated cut-off value was 0.067, and there were no differences in the results of tests that utilized positive and negative sera (p < 0.05). The characteristics of two diagnostic tests were evaluated using a conditional dependent and one-population Bayesian model. The Se value of an in-house indirect ELISA were almost similar to ELISA test kit. On the other hand, the Sp value of the in-house ELISA test was lower than that of the commercial ELISA test with the median values of 89% (95% PPI = 75.9–99.3%) and 91.4% (95% PPI = 85.3–95.5%), respectively. A posterior estimate for the prevalence was 66.9% (95% PPI = 60.8–83.3%) and higher than initially expected. |
| first_indexed | 2024-04-10T06:19:54Z |
| format | Article |
| id | doaj.art-c95952d22c934c06bd2cb69c4c259cc9 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-10T06:19:54Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-c95952d22c934c06bd2cb69c4c259cc92023-03-02T05:01:51ZengElsevierHeliyon2405-84402023-02-0192e13499Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approachNattawooti Sthitmatee0Pallop Tankaew1Wittawat Modethed2Amarin Rittipornlertrak3Anucha Muenthaisong4Nisachon Apinda5Pongpisid Koonyosying6Boondarika Nambooppha7Paweena Chomjit8Kanokwan Sangkakam9Tawatchai Singhla10Paramintra Vinitchaikul11Kittikorn Boonsri12Kidsadagon Pringproa13Veerasak Punyapornwithaya14Khwanchai Kreausukon15Laboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand; Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand; Excellent Center in Veterinary Bioscience, Chiang Mai University, Chiang Mai, Thailand; Corresponding author. Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand.The Veterinary Diagnostic Center, Chiang Mai University Animal Hospital, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandHerd Health Unit, The Fifth Regional Livestock Office, Department of Livestock Development, Ministry of Agriculture and Cooperative, Muang, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand; Ruminant Clinic, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand; Department of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLaboratory of Veterinary Vaccine and Biological Products, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandRuminant Clinic, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandRuminant Clinic, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandThe Veterinary Diagnostic Center, Chiang Mai University Animal Hospital, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandThe Veterinary Diagnostic Center, Chiang Mai University Animal Hospital, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandRuminant Clinic, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, Thailand; Excellent Center for Veterinary Public Health, Chiang Mai University, Chiang Mai, ThailandRuminant Clinic, Department of Food Animal Clinics, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, ThailandLumpy skin disease (LSD) is a contagious disease among cattle and buffalo worldwide. Currently, an enzyme-linked immunosorbent assay (ELISA) has been recognized as an efficient diagnostic tool that is less time-consuming and easier than the viral neutralization test to measure the antibody levels. In the present study, an in-house method of indirect ELISA was developed to detect the bovine antibodies against Lumpy skin disease virus (LSDV) and its performance was assessed using field samples. This in-house method has been compared with the commercial ELISA test kit for detection of bovine antibodies against LSDV. The sensitivity (Se) and the specificity (Sp) of the test were estimated using a Bayesian latent class model. Checkerboard titration was performed using the naturally LSDV-infected bovine sera and colostrum-deprived calf sera. The LSDV antigen concentrations (1 TCID50/mL), the sample serum (1:500), and goat anti-bovine immunoglobulin G (IgG) labeled with horseradish peroxidase (HRP) (1:10,000) were determined to be optimal for this assay. The calculated cut-off value was 0.067, and there were no differences in the results of tests that utilized positive and negative sera (p < 0.05). The characteristics of two diagnostic tests were evaluated using a conditional dependent and one-population Bayesian model. The Se value of an in-house indirect ELISA were almost similar to ELISA test kit. On the other hand, the Sp value of the in-house ELISA test was lower than that of the commercial ELISA test with the median values of 89% (95% PPI = 75.9–99.3%) and 91.4% (95% PPI = 85.3–95.5%), respectively. A posterior estimate for the prevalence was 66.9% (95% PPI = 60.8–83.3%) and higher than initially expected.http://www.sciencedirect.com/science/article/pii/S2405844023007065Bayesian latent class analysisIndirect ELISALumpy skin diseaseSensitivitySpecificity |
| spellingShingle | Nattawooti Sthitmatee Pallop Tankaew Wittawat Modethed Amarin Rittipornlertrak Anucha Muenthaisong Nisachon Apinda Pongpisid Koonyosying Boondarika Nambooppha Paweena Chomjit Kanokwan Sangkakam Tawatchai Singhla Paramintra Vinitchaikul Kittikorn Boonsri Kidsadagon Pringproa Veerasak Punyapornwithaya Khwanchai Kreausukon Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach Heliyon Bayesian latent class analysis Indirect ELISA Lumpy skin disease Sensitivity Specificity |
| title | Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach |
| title_full | Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach |
| title_fullStr | Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach |
| title_full_unstemmed | Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach |
| title_short | Development of in-house ELISA for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach |
| title_sort | development of in house elisa for detection of antibodies against lumpy skin disease virus in cattle and assessment of its performance using a bayesian approach |
| topic | Bayesian latent class analysis Indirect ELISA Lumpy skin disease Sensitivity Specificity |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023007065 |
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