Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China.
To make better use of global germplasm resources for improving the eating quality of hybrid rice, using the resequencing data from the 3,000 rice genomes project (3K RGP), the allelic variations of the rice Wx locus were analysed. With the exception of five rare alleles discovered for the first time...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0232279 |
| _version_ | 1818677994102718464 |
|---|---|
| author | Ye Shao Yan Peng Bigang Mao Qiming Lv Dingyang Yuan Xionglun Liu Bingran Zhao |
| author_facet | Ye Shao Yan Peng Bigang Mao Qiming Lv Dingyang Yuan Xionglun Liu Bingran Zhao |
| author_sort | Ye Shao |
| collection | DOAJ |
| description | To make better use of global germplasm resources for improving the eating quality of hybrid rice, using the resequencing data from the 3,000 rice genomes project (3K RGP), the allelic variations of the rice Wx locus were analysed. With the exception of five rare alleles discovered for the first time in our study, most of these alleles were known alleles of Wx. Furthermore, a set of Kompetitive allele-specific PCR (KASP) markers based on these Wx alleles have been developed, and thirty-six main parents of hybrid rice from 1976 to 2018 were selected for Wx genotyping. The results showed that only three Wx alleles existed in the main parents of hybrids, and the allelic combination of the hybrids changed from Wxa/Wxb and Wxlv/Wxb to Wxb/Wxb with the development of hybrid rice. Wxb is widely used in the male parents of hybrid rice. Wxa and Wxlv were used in the female parents of early hybrid rice, and they were gradually replaced by Wxb. In the future, more favourable Wx alleles from cultivated rice should be identified, introduced, and effectively used to improve hybrid rice quality. |
| first_indexed | 2024-12-17T09:08:12Z |
| format | Article |
| id | doaj.art-c95feb532fee44029eaf006c7ed806b1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T09:08:12Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c95feb532fee44029eaf006c7ed806b12022-12-21T21:55:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-01155e023227910.1371/journal.pone.0232279Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China.Ye ShaoYan PengBigang MaoQiming LvDingyang YuanXionglun LiuBingran ZhaoTo make better use of global germplasm resources for improving the eating quality of hybrid rice, using the resequencing data from the 3,000 rice genomes project (3K RGP), the allelic variations of the rice Wx locus were analysed. With the exception of five rare alleles discovered for the first time in our study, most of these alleles were known alleles of Wx. Furthermore, a set of Kompetitive allele-specific PCR (KASP) markers based on these Wx alleles have been developed, and thirty-six main parents of hybrid rice from 1976 to 2018 were selected for Wx genotyping. The results showed that only three Wx alleles existed in the main parents of hybrids, and the allelic combination of the hybrids changed from Wxa/Wxb and Wxlv/Wxb to Wxb/Wxb with the development of hybrid rice. Wxb is widely used in the male parents of hybrid rice. Wxa and Wxlv were used in the female parents of early hybrid rice, and they were gradually replaced by Wxb. In the future, more favourable Wx alleles from cultivated rice should be identified, introduced, and effectively used to improve hybrid rice quality.https://doi.org/10.1371/journal.pone.0232279 |
| spellingShingle | Ye Shao Yan Peng Bigang Mao Qiming Lv Dingyang Yuan Xionglun Liu Bingran Zhao Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China. PLoS ONE |
| title | Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China. |
| title_full | Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China. |
| title_fullStr | Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China. |
| title_full_unstemmed | Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China. |
| title_short | Allelic variations of the Wx locus in cultivated rice and their use in the development of hybrid rice in China. |
| title_sort | allelic variations of the wx locus in cultivated rice and their use in the development of hybrid rice in china |
| url | https://doi.org/10.1371/journal.pone.0232279 |
| work_keys_str_mv | AT yeshao allelicvariationsofthewxlocusincultivatedriceandtheiruseinthedevelopmentofhybridriceinchina AT yanpeng allelicvariationsofthewxlocusincultivatedriceandtheiruseinthedevelopmentofhybridriceinchina AT bigangmao allelicvariationsofthewxlocusincultivatedriceandtheiruseinthedevelopmentofhybridriceinchina AT qiminglv allelicvariationsofthewxlocusincultivatedriceandtheiruseinthedevelopmentofhybridriceinchina AT dingyangyuan allelicvariationsofthewxlocusincultivatedriceandtheiruseinthedevelopmentofhybridriceinchina AT xionglunliu allelicvariationsofthewxlocusincultivatedriceandtheiruseinthedevelopmentofhybridriceinchina AT bingranzhao allelicvariationsofthewxlocusincultivatedriceandtheiruseinthedevelopmentofhybridriceinchina |